01/09/1994–01/06/2000 Ege Üniversitesi Tıp Fakültesi, İzmir
01/11/2013–2019 Batı Anadolu Central Hospital, Uzman Doktorluk ve İnvazif Yoğun Bakım Ünitesi Yönetici Doktor, İzmir
01/08/2011–01/09/2013 Uzman Doktor, Universal Hastaneler Grubu, Kuşadası
01/09/2006–01/06/2011 Uzman Doktor, Çarşamba Devlet Hastanesi, Samsun
01/05/2006–30/08/2006 Uzman Doktor, Ege Üniversitesi Tıp Fakültesi Göğüs Hastalıkları A.D., İzmir
01/10/2000–30/04/2006 Asistan Doktor, Ege Üniversitesi Tıp Fakültesi Göğüs Hastalıları A.D., İzmir
By
Biyikli, OO (Biyikli, Oguz Oben) [1] ; Baysak, A (Baysak, Aysegul) [2] ; Ece, G (Ece, Gulfem) [3] ; Oz, AT (Oz, Adnan Tolga) [2] ; Ozhan, MH (Ozhan, Mustafa Hikmet) [4] ; Berdeli, A (Berdeli, Afig) [5]
(provided by Clarivate)
Source
JUNDISHAPUR JOURNAL OF MICROBIOLOGY
Volume
9
Issue
10
DOI
10.5812/jjm.20224
Article Number
e20224
Published
OCT 2016
Indexed
2017-02-21
Document Type
Article
Abstract
Background: One-third of the world's population is infected with Mycobacterium tuberculosis. Investigation of Toll-like receptors (TLRs) has revealed new information regarding the immunopathogenesis of this disease. Toll-like receptors can recognize various ligands with a lipoprotein structure in the bacilli. Toll-like receptor 2 and TLR-4 have been identified in association with tuberculosis infection.
Objectives: The aim of our study was to investigate the relationship between TLR polymorphism and infection progress.
Methods: Twenty-nine patients with a radiologically, microbiologically, and clinically proven active tuberculosis diagnosis were included in this 25-month study. Toll-like receptor 2 and TLR-4 polymorphisms and allele distributions were compared between these 29 patients and 100 healthy control subjects. Peripheral blood samples were taken from all patients. Genotyping of TLR-2, TLR-4, and macrophage migration inhibitory factor was performed. The extraction step was completed with a Qiagen mini blood purification system kit (Qiagen, Ontario, Canada) using a peripheral blood sample. The genotyping was performed using polymerase chain reaction-restriction fragment length polymorphism.
Results: In total, 19 of the 29 patients with tuberculosis infection had a TLR-2 polymorphism, and 20 of the 100 healthy subjects had a TLR-2 polymorphism (P < 0.001). The TLR-4 polymorphism and interferon-gamma allele distributions were not statistically correlated.
Conclusions: Toll-like receptor 2 polymorphism is a risk factor for tuberculosis infection. The limiting factor in this study was the lack of investigation of the interferon-gamma and tumor necrosis factor-ff levels, which are important in the development of infection. Detection of lower levels of these cytokines in bronchoalveolar lavage specimens, especially among patients with TLR-2 defects, will provide new data that may support the results of this study.
Keywords
Author Keywords
Toll-Like Receptor Infection Genetic Polymorphism Mycobacterium tuberculosis