YÖNETİLEN YÜKSEK LİSANS VE DOKTORA TEZLERİ

YÜKSEK LİSANS TEZLERİ              

1. ÇOPUR ÖZNUR, (2022). İdrarda total glikozaminoglikan semi-kantitatif ölçümü için hızlı test yöntemi geliştirilmesi, Ege Üniversitesi->Sağlık Bilimleri Enstitüsü->Tıbbi Biyokimya Ana Bilim Dalı (Tamamlandı)               

2. VURAL MELİSA RABİA, (2021). Gaucher hastalığının tanısı ve şiddetinin göstergesi olarak pteridin metabolitlerinin araştırılması, Ege Üniversitesi->Sağlık Bilimleri Enstitüsü->Tıbbi Biyokimya Ana Bilim Dalı (Tamamlandı)                                                  

3. DURMAZ BURAK, (2018). Yaban mersininde bulunan hidroksisinnamik asidlerin antioksidan aktivitesi ve kanser hücre kültüründe apopitozis induksiyonu üzerine etkisi, Ege Üniversitesi->Sağlık Bilimleri Enstitüsü->Tıbbi Biyokimya Ana Bilim Dalı (Tamamlandı)                                                  

4. DONDURMACI MERAL, (2017). Lizozomal depo hastalıklarının lökositlerinde  hastalık ile ilgili spesifik enzim aktivitelerinin ölçülmesi, Ege Üniversitesi->Sağlık Bilimleri Enstitüsü->Biyokimya Ana Bilim Dalı (Tamamlandı)

5. ERGÜN PELİN, (2014). Lizozomal depo hastalıklarının tanınması ve izleniminde yeni inflamasyon belirteçlerinin araştırılması, Ege Üniversitesi->Sağlık Bilimleri Enstitüsü->Tıbbi Biyokimya Ana Bilim Dalı (Tamamlandı)

DOKTORA TEZLERİ                                         

1. DURMAZ BURAK, (2024). Doğal Kaynaklardan Dao (Diamin Oksidaz) Pürifikasyonu ve Enzimle Zenginleştirilmiş Gıda Üretimi, Ege Üniversitesi->Sağlık Bilimleri Enstitüsü->Tıbbi Biyokimya Ana Bilim Dalı (Tamamlandı)

2. ERGÜN KARAKAŞLI PELİN, (2021). Gastroözofageal reflü hastalığının altgruplarında inflamasyon mekanizmalarının araştırılması, Ege Üniversitesi->Sağlık Bilimleri Enstitüsü->Tıbbi Biyokimya Ana Bilim Dalı (Tamamlandı)                                                  

3. MAMMADOV HIKMAT, (2019). Biyoteknolojik transformasyon ile alerjen molekül içeriği azaltılmış propolisin kanser hücrelerinde apopitozis ve  anjiogenez üzerine etkisi, Ege Üniversitesi->Sağlık Bilimleri Enstitüsü->Tıbbi Biyokimya Ana Bilim Dalı (Tamamlandı)

4. ÖZTÜRK ŞAHİN, (2019). Mersin (Myrtus Communis l.) bitkisinin farklı ekstraktlarının fenolik içeriğinin belirlenmesi ve hücre kültüründe antioksidan – antiinflamatuar etkilerinin araştırılması, Ege Üniversitesi->Sağlık Bilimleri Enstitüsü->Tıbbi Biyokimya Ana Bilim Dalı (Tamamlandı)

5. ALDEMİR OZAN, (2016). Propolisten alerjik etkisi düşük yeni bir biyoteknolojik ürün eldesi ve bu ürünün antienflamatuvar etkisinin araştırılması, Ege Üniversitesi->Fen Bilimleri Enstitüsü->Biyoteknoloji Ana Bilim Dalı (Tamamlandı)

TIPTA UZMANLIK TEZLERİ                                          

1. KOLOĞLU TURAN, (2010). Dislipidemik çocuklarda makrofaj aktivasyonunun göstergesi olarak serum kitotriozidaz aktivitesinin ölçümü, Ege Üniversitesi->Tıp Fakültesi->Tıbbi Biyokimya Ana Bilim Dalı (Tamamlandı)                                  

2. İLANBEY BİLAL, (2006). Genç yaşlarda görülen aterosklerotik kalp hastalığında CETP Taq1B polimorfizminin rolü, Ege Üniversitesi->Tıp Fakültesi->Biyokimya Ana Bilim Dalı (Tamamlandı)

3. AZARSIZ ELİF, (2001). Koroner arter hastalığı olgularında LDL oksidasyonu ve risk faktörü olarak paraoksonaz fenotipinin değerlendirilmesi, Ege Üniversitesi->Tıp Fakültesi->Biyokimya Ana Bilim Dalı (Tamamlandı)

 YAYINLAR

ULUSLARARASI KİTAPLAR VEYA KİTAPLARDAKİ BÖLÜMLER

1. Methods for determination of alpha-glycosidase, ß-glycosidase and alpha-galactosidase activities in dried blood spot samples., Methods in Molecular Biology: Lysosomes methods and protocols., Chapter Research Book, Springer, London/Berlin , A, 2017, pp 255-264, England
2.  Good Practices In Home Care in Turkey, Good Practices And Visions Of The Future Of Home Care Work In Bulgaria, Finland, Greece And Turkey, Chapter Research Book, City of Turku, Education Department and Turku Vocational Institute, 2012, pp 29-34, Finland
3. Comparisons of Home Care Work In Bulgaria, Finland, Greece and Turkey, Good Practices And Visions Of The Future Of Home Care Work In Bulgaria, Finland, Greece And Turkey, Chapter Research Book, City of Turku, Education Department and Turku Vocational Institute, Other, 2012, pp 37- 53, Finland
4. Oxidative stress and antioxidants and PON1 in health and disease, The paraoxonases: their role in disease development and xenobiotic metabolism, Chapter Research Book, Springer-Verlag, B, 2008, pp 124-135, Netherlands
5.  Link Between Monoamine Oxidase and Nitric Oxide, Monoamine Oxidases Molecular Pharmacological and Neurotoxicological Aspects, Chapter Vocational Book, Elsevier Press, 2004, pp 91-99
6. The Relationship between Plasma Antioxidant Status, Nitrate Levels, and Lipid Profile in Patients with Hypertension and Coronary Heart Disease, Vascular Endothelium., Chapter Other Book, Springer-Verlag, B, 1998, pp 275-276, USA

Effect of Propolis on PPP2R1A and Apoptosis in Cancer Cells

By

Durmaz, B (Durmaz, Burak) [1] , [2] ; Çelebi, LMO (Celebi, Latife Merve Oktay) [3] ; Çekin, A (Cekin, Ayse) [3] ; Ahadova, A (Ahadova, Ayshan) [3] ; Günel, NS (Gunel, Nur Selvi) [3] ; Yildirim, HK (Yildirim, Hatice Kalkan) [4] ; Özgönül, AM (Ozgonul, Ali Mert) [2] ; Sözmen, EY (Sozmen, Eser Yildirim) [2] , [5]

 (provided by Clarivate) 

Source

BIOCHEMISTRY RESEARCH INTERNATIONAL

Volume

2025

Issue

1

DOI

10.1155/bri/5538068

Article Number

5538068

Published

2025

Indexed

2025-01-19

Document Type

Article

Abstract

Recently, it has been shown that protein phosphatase 2A (PP2A) dysfunction was common in many cancer types and was mediated by various inactivation mechanisms. Although many research studies observed antitumor effect of propolis extracts in various types of cancer, the mechanism of effect are still obscure. In this study, we investigated the effect of propolis on PPP2R1A expression and its relationship with apoptosis in the SW-620 (colorectal cancer), DU-145 and PC-3 (prostate cancer), and MCF-7 (breast cancer) cell lines, with WI-38 (healthy fibroblast) cells serving as the control. Moreover, we aimed to investigate the impact of propolis on apoptosis by analyzing apoptosis markers such as tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), APAF-1, and caspases-3, -8, and -9. Propolis samples were extracted, and their phenolic compounds were quantified using LC-MS/MS. The RealTime Cell Analysis System-xCELLigence (RTCA-SP) device and software were employed to assess cell viability and cytotoxicity of the propolis samples. The IC50 values for propolis were determined (298 mu g/mL for SW-620, 185.6 mu g/mL for DU-145, 250.7 mu g/mL for PC - 3, 292.9 mu g/mL for MCF-7, and 311.2 mu g/mL for WI-38). Subsequently, the effects of propolis on PPP2R1A expression and apoptosis markers (TRAIL, Apaf-1, and caspases-3, -8, and -9) were analyzed. When we compared the healthy cell lines to cancer cell lines, a statistically significant increase in caspase-3 (3.62-fold) and in TRAIL (4.38-fold) was observed in the SW-620 cell line after the application of propolis. In addition, in the PC-3 cell line, a 1.4-fold increase in caspase-8 was observed compared with the healthy cell line, which is also statistically significant. Our findings indicated that propolis increased the PPP2R1A levels and apoptosis markers in cancer cell lines. It has been suggested that high PPP2R1A levels induced by propolis treatment might activate the apoptosis pathway. In this study, the inducible effect of propolis on PPP2R1A levels, identified as a new target for cancer treatment, was demonstrated for the first time. The findings suggest that propolis holds promise as a potential cancer therapy by increasing PPP2R1A levels, a key molecule in cancer treatment.

Keywords

Author Keywords

apoptosis metastasis propolis tumor suppressor

Determination of selected oxysterol levels, oxidative stress, and macrophage activation indicators in children and adolescents with familial hypercholesterolemia

By

Canbay, E (Canbay, Erhan) [1] ; Canda, E (Canda, Ebru) [2] ; Yazici, H (Yazici, Havva) [2] ; Kasikci, GK (Kasikci, Gulcin Kayan) [3] ; Durmaz, B (Durmaz, Burak) [1] , [6] ; Copur, O (Copur, Oznur) [1] , [7] ; Tahhan, B (Tahhan, Begum) [1] ; Duzgun, D (Duzgun, Dilek) [4] ; Koru, ZE (Koru, Zeynep Elcim) [4] ; Sezer, E (Sezer, Ebru) [1] ; 

 (provided by Clarivate) 

Source

LIPIDS IN HEALTH AND DISEASE

Volume

23

Issue

1

DOI

10.1186/s12944-024-02371-y

Article Number

374

Published

NOV 13 2024

Indexed

2024-11-21

Document Type

Article

Jump to

Abstract

Aim Elevated levels of cholesterol in the bloodstream, also referred to as hypercholesterolemia, pose a significant risk for the onset of cardiovascular and cerebrovascular diseases. Oxysterols, cholesterol-derived oxidized compounds that form enzymatically or non-enzymatically, contribute to the development of atherosclerosis and coronary artery disease. This study aimed to examine the critical oxysterol levels in children and adolescents with hypercholesterolemia and explore the correlation between these levels, oxidative stress, and atherosclerosis progression. Materials and methods The study included 20 patients with familial hypercholesterolemia (FH) and 20 healthy individuals aged between 6 and 18 years. Participants were categorized into children (6-9 years) and adolescents (10-18 years). Pediatric and adolescent patients were selected from among subjects with LDL-C >= 130 mg/dL and diagnosed with heterozygous familial hypercholesterolemia (HeFH) based on the presence of mutations in the LDL receptor (LDL-R) gene. Patients with HeFH who were receiving regular atorvastatin therapy were included in the study. Results There were no notable differences in catalase and paraoxonase (PON1) activities among the groups. However, the patient group displayed substantially higher levels of malondialdehyde (MDA) (P = 0.0108) and superoxide dismutase (SOD) activity (P = 0.0103). Compared to the healthy control group, serum chitotriosidase (CHITO) activity (P = 0.037) and chitinase 3-like protein 1 (YKL-40) levels (P = 0.0027) were significantly elevated in the patient group. Furthermore, the carotid intima-media thickness (CIMT) measurements of the patient group were significantly greater than those of the healthy group (**P < 0.0001****). The patient group exhibited significantly elevated levels of 5,6-alpha-epoxycholesterol, Cholestane-3 beta,5 alpha,6 beta-triol (C-triol), and 7-ketocholesterol (7-KC), whereas 27-hydroxycholesterol (27-OHC) was significantly more abundant in the healthy group. On the other hand, while 27-OHC/Total cholesterol (Total-C) levels were significantly higher in healthy individuals, the C-Triol/Total-C ratio was significantly higher in patients. No significant differences were found between the groups in terms of 7-KC/Total-C and 5,6-alpha-epoxycholesterol/Total-C levels. Conclusion This study highlights the key roles of oxysterols, oxidative stress, and macrophage activation in the development of atherosclerosis in pediatric and adolescent patients with FH. Elevated C-Triol levels in FH patients, alongside increased CIMT, point to early vascular changes despite atorvastatin therapy. In contrast, higher 27-OHC levels in healthy controls suggest differential oxysterol regulation due to cholesterol-lowering treatments in FH patients. C-Triol and 27-OHC/Total-C ratios showed potential as biomarkers to distinguish patients with FH. These findings emphasize the need for therapies targeting oxidative stress and macrophage activation in addition to cholesterol-lowering interventions.

Keywords

Author Keywords

Hypercholesterolemia Atherosclerosis CIMT Oxysterol Oxidative stress Macrophage activation

Development of a New Amperometric Biosensor for Measurement of Plasma Galactose Levels

By

Canbay, E (Canbay, Erhan) [1] ; Sezer, E (Sezer, Ebru) [1] ; Canda, E (Canda, Ebru) [2] ; Yazici, H (Yazici, Havva) [2] ; Uçar, SK (Ucar, Sema Kalkan) [2] ; Çoker, M (Coker, Mahmut) [2] ; Sözmen, EY (Sozmen, Eser Yildirim) [1]

 (provided by Clarivate) 

Source

ACS OMEGA

Volume

9

Issue

7

Page

7621-7633

DOI

10.1021/acsomega.3c06789

Published

FEB 7 2024

Indexed

2024-03-11

Document Type

Article

Jump to

Abstract

Galactosemia is an inherited disease that occurs as a result of insufficient or no synthesis of some enzymes (GALT, GALK, and GALE) in galactose metabolism. Failure to make an early diagnosis, especially in newborns, can lead to severe clinical and even fatal consequences. The aim of this study is to develop a biosensor for measuring free galactose in plasma. The immobilization components of the developed free galactose biosensor are screen printed carbon electrode (SCPE), Prussian blue (PB), chitosan (CHIT), Nafion (NAF), gold nanoparticle (GNP), and galactose oxidase (GaOX). The CHIT/GaOX/NAF-GNP/GaOX/CHIT-GNP/SCPE-PB electrode showed a sensitive amperometric response to detect galactose. While the surface characterization of the biosensor was performed with cyclic voltammetry and scanning electron microscopy, the optimization and performance characterizations were made by applying an amperometry technique. The amperometric operating potential for the free galactose biosensor was determined as -0.05 V. The linear detection range for the free galactose biosensor is between 0.025 and 10 mM. This range includes galactose levels in plasma of both healthy and patients. The percent coefficient of variation values calculated for intraday and interday repeatability of the developed biosensor are below 10%. The practical use of the biosensor, for which optimization and characterization studies were carried out, was tested in 10 healthy 11 patients with galactosemia, and the results were compared with the colorimetric method. In conclusion, the unique analytical properties and effortless preparation of the new galactose biosensor developed in this study make them serious candidates for point-of-care diagnostic testing.

Keywords

Keywords Plus

HYDROGEN-PEROXIDECARBON NANOTUBESGLUCOSE-OXIDASEPRUSSIAN BLUE CHITOSAN

Dao (diaminoxidase) purification from natural sources and enzyme-enriched food production

By

Durmaz, B (Durmaz, Burak) [1] , [4] ; Canbay, E (Canbay, Erhan) [1] ; Baykan, SÖ (Baykan, Sura Ozturk) [2] ; Yildirim, HK (Yildirim, Hatice Kalkan) [3] ; Sözmen, EY (Sozmen, Eser Yildirim) [5]

 (provided by Clarivate) 

Source

APPLIED FOOD RESEARCH

Volume

5

Issue

2

DOI

10.1016/j.afres.2025.101155

Article Number

101155

Published

DEC 2025

Early Access

JUL 2025

Indexed

2025-08-02

Document Type

Article

Abstract

Histamine intolerance, affecting approximately 1-3 % of the population, is linked to elevated histamine levels that can mimic allergic reactions. Diamine oxidase (DAO) plays a key role in histamine degradation. This study aimed to purify a plant-derived DAO enzyme with higher specific activity than commercial supplements and to apply it in fermented foods, specifically pickles, widely consumed in Turkish cuisine. DAO was extracted from wheatgrass produced from a local cultivar registered by the Aegean Agricultural Research Institute. The enzyme was purified using ammonium sulfate precipitation and dialysis. Enzyme activity and histamine levels were quantified using LC/MS-MS. Wheatgrass DAO showed a specific activity of 10.18 U/ mg in the crude extract and increased to 207.6 U/mg after dialysis. To assess its functionality, DAO (both commercial and plant-derived) was added at various concentrations during pickle fermentation. After three weeks, herbal DAO reduced histamine levels in pickle juice by up to 96 %, significantly outperforming the commercial enzyme in most cases. This is the first reported application of wheatgrass-derived DAO in food fermentation (Patent No: P-201,710). The findings suggest a promising natural solution for reducing histamine in fermented foods, offering a dietary alternative for individuals with histamine intolerance.

Keywords

Author Keywords

Diamin oxidase  Histamine Allergy Histamine intorelance Food Enrichment

Validation of New Micro-spectrophotometric Method to Determine Diamine Oxidase Activity in Serum

By

Sozmen, EY (Sozmen, Eser Yildirim) [2] , [3] ; Sozmen, AB (Sozmen, Alper Baran) [1]

 (provided by Clarivate) 

Source

INDIAN JOURNAL OF CLINICAL BIOCHEMISTRY

DOI

10.1007/s12291-024-01253-5

Early Access

JUL 2024

Indexed

2024-08-02

Document Type

Article; Early Access

Jump to

Abstract

Food allergy and histamine intolerance (HI) are prevalent worldwide. Diamine oxidase (DAO) activity determination in serum is a promising approach for diagnosing HI. Despite the numerous methods that have been published for determining diamine. oxidase activity in serum, most of them have not been validated and have inherent limitations, such as interference and multi-step analysis. Our objective was to perform the analytical validation of a novel micro-spectrophotometric method for the determination of DAO activity in a routine clinical laboratory setting. In this study we determined DAO activity in serum using a 4-aminoantypirine (4-AAP) and 2,4,6 tribromo-3-hidroxybenzoic acid (TBHBA) combination as chromogen for the first time. Validation steps included determination of accuracy (recovery and linearity) and precision (within-run and between-run repeatability) following the Clinical and Laboratory Standards Institute protocols and the other guidelines. Reagent stability assessment at both 4 degrees C temperature and frozen (- 20 degrees C) after 4 h, 24 h, 72 h and a week storage were conducted. The optimum test conditions were determined as pH = 6.0, 37 degrees C, 55 mM Putrescine and 60 min. LOD and LOQ levels were found as 10.08 U/L and 30.56 U/L respectively. Coefficients of variation (CVs) for within-run and between-run precisions were 5.2%, 9.4% for the normal level and 2.6%, 2.2% for the high range, respectively. This reliable method for quantifying DAO activity in serum provides an optimal approach, offering rapid and straightforward performance in laboratories for clinical use.

Keywords

Author Keywords

Diamine oxidase Histamine intolerance Food allergy

Inflammatory responses in esophageal mucosa before and after laparoscopic antireflux surgery

By

Ergun, P (Ergun, Pelin) [1] , [2] , [5] ; Kipcak, S (Kipcak, Sezgi) [2] , [3] ; Gunel, NS (Gunel, Nur Selvi) [3] ; Sozmen, EY (Sozmen, Eser Yildirim) [4] ; Bor, S (Bor, Serhat) [2]

 (provided by Clarivate) 

Source

WORLD JOURNAL OF GASTROINTESTINAL SURGERY

Volume

16

Issue

3

DOI

10.4240/wjgs.v16.i3.871

Published

MAR 27 2024

Indexed

2024-04-24

Document Type

Article

Jump to

Abstract

BACKGROUND Currently, the primary treatment for gastroesophageal reflux is acid suppression with proton pump inhibitors, but they are not a cure, and some patients don't respond well or refuse long-term use. Therefore, alternative therapies are needed to understand the disease and develop better treatments. Laparoscopic anti-reflux surgery (LARS) can resolve symptoms of these patients and plays a significant role in evaluating esophageal healing after preventing harmful effects. Successful LARS improves typical gastroesophageal reflux symptoms in most patients, mainly by reducing the exposure time to gastric contents in the esophagus. Amelioration of the inflammatory response and a recovery response in the esophageal epithelium is expected following the cessation of the noxious attack. AIM To explore the role of inflammatory biomolecules in LARS and assess the time required for esophageal epithelial recovery. METHODS Of 22 patients with LARS (pre- and post/5.8 +/- 3.8 months after LARS) and 25 healthy controls (HCs) were included. All subjects underwent 24-h multichannel intraluminal impedance-pH monitoring and upper gastrointestinal endoscopy, during which esophageal biopsy samples were collected using endoscopic techniques. Inflammatory molecules in esophageal biopsies were investigated by reverse transcription-polymerase chain reaction and multiplex-enzyme-linked immunosorbent assay. RESULTS Post-LARS samples showed significant increases in proinflammatory cytokines [interleukin (IL)-1 beta, interferon-gamma, C-X-C chemokine ligand 2 (CXCL2)], anti-inflammatory cytokines [CC chemokine ligand (CCL) 11, CCL13, CCL17, CCL26, CCL1, CCL7, CCL8, CCL24, IL-4, IL-10], and homeostatic cytokines (CCL27, CCL20, CCL19, CCL23, CCL25, CXCL12, migration inhibitory factor) compared to both HCs and pre-LARS samples. CCL17 and CCL21 levels were higher in pre-LARS than in HCs (P < 0.05). The mRNA expression levels of AKT1, fibroblast growth factor 2, HRAS, and mitogen-activated protein kinase 4 were significantly decreased post-LARS vs pre-LARS. CCL2 and epidermal growth factor gene levels were significantly increased in the pre-LARS compared to the HCs (P < 0.05). CONCLUSION The presence of proinflammatory proteins post-LARS suggests ongoing inflammation in the epithelium. Elevated homeostatic cytokine levels indicate cell balance is maintained for about 6 months after LARS. The anti-inflammatory response post-LARS shows suppression of inflammatory damage and ongoing postoperative recovery.

Keywords

Author Keywords

Anti-reflux surgery Gastroesophageal reflux disease Cytokine Inflammatory response Esophagus

Insights into skeletal involvement in adult Gaucher disease: a single-center experience

By

Çelik, MY (celik, Merve Yoldas) [1] ; Canda, E (Canda, Ebru) [1] ; Yazici, H (Yazici, Havva) [1] ; Erdem, F (Erdem, Fehime) [1] ; Yanbolu, AY (Yanbolu, Ayse Yuksel) [1] ; Aykut, A (Aykut, Ayca) [2] ; Durmaz, A (Durmaz, Asude) [2] ; Uçar, SK (Ucar, Sema Kalkan) [1] ; Sözmen, EY (Sozmen, Eser Yildirim) [3] ; Çoker, M (Coker, Mahmut) [1]

 (provided by Clarivate) 

Source

JOURNAL OF BONE AND MINERAL METABOLISM

Volume

43

Issue

2

Page

166-173

DOI

10.1007/s00774-024-01573-9

Published

MAR 2025

Early Access

JAN 2025

Indexed

2025-01-24

Document Type

Article

Jump to

Abstract

Introduction Gaucher disease (GD) is a lysosomal storage disorder causing systemic and skeletal complications. This study evaluates bone health in adult GD type 1 patients, focusing on skeletal complications, bone mineral density (BMD), and biochemical markers. Material and methods A cohort of adult GD type 1 patients followed up at Ege University Pediatric Metabolism Department were retrospectively examined. Results This study included 32 patients with GD type 1, comprising 11 males (34.4%) and 21 females (65.6%). The median age at diagnosis was 20.5 years (min: 3-max:65), and at enrolment, it was 35 years (min:18-max:71). Most patients (93.8%) had organomegaly, and 93.8% had cytopenia. Common genetic variants were p.Asn409Ser (60.9%), p.Leu483Pro (7.8%), and p.Asp448His(4.7%). All patients were on enzyme replacement therapy (ERT) for a median of 11 years (min:2-max:18). Bone complications included pathologic fractures in six patients (19%) and avascular necrosis in 12 patients (37.5%). Bone pain was reported by 93.7% of patients at admission and persisted in 59.4% during follow-up. DXA scans showed abnormal bone mineral density (BMD) in 62.5% of patients initially, with a significantly low bone density in 3.1% and reduced bone density in 59.3%. BMD improved with treatment, as evidenced by a significant increase in Z scores (p < 0.05). Elevated chitotriosidase (75%), ferritin (50%), and immunoglobulin G (21.9%) levels were noted but did not correlate with BMD. Seven patients (22%) were splenectomized, all with bone issues. Discussion Bone health in GD involves multiple factors beyond biochemical markers. While ERT improves BMD, bone pain and fractures remain significant issues. Comprehensive management, including regular BMD monitoring and better vitamin D supplementation adherence, is crucial. Further research is needed to improve treatments for bone complications in GD.

Keywords

Author Keywords

Gaucher disease Bone mineral density Skeletal involvement Pathological fractures Osteopenia

Experimental study comparing the effects of cold and warm applications on oxidative stress markers in a rat extremity ischemia-reperfusion injury model

By

Erol, K (Erol, Kubilay) [1] ; Vahabi, A (Vahabi, Arman) [2] ; Er, E (Er, Erdem) [1] ; Sözmen, EY (Sozmen, Eser Yildirim) [3] ; Kücük, L (Kucuk, Levent) [4]

 (provided by Clarivate) 

Source

ULUSAL TRAVMA VE ACIL CERRAHI DERGISI-TURKISH JOURNAL OF TRAUMA & EMERGENCY SURGERY

Volume

31

Issue

5

Page

425-430

DOI

10.14744/tjtes.2025.82421

Published

MAY 2025

Indexed

2025-06-13

Document Type

Article

Jump to

Abstract

BACKGROUND: After successful restoration of limb perfusion following limb ischemia, a series of secondary damage mechanisms, collectively known as reperfusion injury, is triggered. This study investigated the comparative effects of warming, a technique commonly used in clinical settings after extremity revascularization, and cooling, in a rat model of ischemia-reper fusion injury. METHODS: Sprague Dawley rats aged 12 to 14 weeks and weighing between 250-350 g were used. The study included four experimental groups: Sham, Control, Cold Application, and Warm Application. Ischemia was induced by occluding the femoral artery with an atraumatic vascular clamp for three hours. Cold and warm treatments were applied for two hours by immersing the hind limbs in water at specific temperatures. In the cold application group, water temperature was maintained at 12 +/- 2 degrees C using ice cubes to lower the temperature as needed. In the warm application group, the water temperature was kept at 38 +/- 2 degrees C by adding hot water when necessary. Twenty-four hours after the injury model was established, the animals underwent a second surgical procedure to obtain tissue samples for analyses of malondialdehyde (MDA), myeloperoxidase (MPO), poly(ADP-ribose) polymerase (PARP), catalase, and superoxide dismutase (SOD). RESULTS: Compared to the control group, the warm application group showed no statistically significant differences in levels of MDA (p=0.910), MPO (p=0.527), PARP (p=0.192), catalase (p=0.999), or SOD (p=0.987). In contrast, the cold application group exhibited a significant reduction in MDA, MPO, PARP, catalase, and SOD levels (p<0.001 for all). CONCLUSION: To minimize reperfusion injury following limb ischemia, cold application may provide greater benefits than warming the extremity. Further studies are necessary to explore the clinical relevance and applications of this finding.

Keywords

Author Keywords

Amputation cold cooling crush hypothermia microsurgery revascularization warm

Perspective on pros and cons of microlearning in health education

By

Sozmen, EY (Sozmen, Eser Yildirim) [1]

 (provided by Clarivate) 

Source

ESSAYS IN BIOCHEMISTRY

Volume

66

Issue

1

Page

39-44

Special Issue

SI

DOI

10.1042/EBC20210047

Published

APR 2022

Indexed

2022-07-28

Document Type

Review

Abstract

Recently, digital technology and digital materials have started to be widely used in education from primary school to college worldwide. Microlearning is one of the innovative teaching techniques that use digital technologies. In this review, benefits and disadvantages of microlearning is discussed. Many studies show that microlearning facilitated learning by dividing into smaller pieces encourages students to study. A wide range of activities might be used in this technique and it can be easily integrated into daily routine, it allows on-demand learning for the students. On the other hand, the success of microlearning techniques is closely related to the personal characteristics of learners, teachers' prone to use digital technology and the external factors such as access to learning materials. Its effectiveness on behavior and outcome which were defined in the third and fourth levels of Kirkpatrick's learning model is still obscure.

In the light of the literature, it should be decided which microlearning method will be used for which educational subjects.

Ocular Manifestations of Fabry Disease: Report from a Tertiary Eye Care Center in Türkiye

By

Korkmaz, I (Korkmaz, Ilayda) [1] ; Uçar, SK (Ucar, Sema Kalkan) [2] ; Onay, H (Onay, Huseyin) [3] ; Sözmen, EY (Sozmen, Eser Yildirim) [4] ; Çoker, M (Coker, Mahmut) [2] ; Palamar, M (Palamar, Melis) [1]

 (provided by Clarivate) 

Source

TURK OFTALMOLOJI DERGISI-TURKISH JOURNAL OF OPHTHALMOLOGY

Volume

54

Issue

3

Page

127-132

DOI

10.4274/tjo.galenos.2024.09482

Published

JUN 2024

Indexed

2024-08-01

Document Type

Article

Abstract

Objectives: To report ocular manifestations in patients with Fabry disease (FD) from a tertiary eye care center in T & uuml;rkiye. Materials and Methods: This prospective, cross-sectional study included 30 eyes of 15 patients with FD. The diagnosis of FD was made based on a combination of clinical findings, genetic analysis, and biochemical evaluation. All participants underwent a detailed ophthalmic examination with special focus on the typical ocular features of FD (cornea verticillata, conjunctival aneurysms, cataract, retinal vessel tortuosity). Results: The mean age was 45 +/- 17 years (range: 22-75 years), with a female/male ratio of 2:3. All patients had tortuous conjunctival vessels and 12 patients (80%) had conjunctival aneurysms. Cornea verticillata was present in 10 patients (66.6%), lens opacification in 4 patients (26.6%), and retinal vascular tortuosity in 8 patients (53.3%). All patients had at least two different ocular findings; most (3 heterozygotes/7 hemizygotes) had a combination of corneal verticillata and conjunctival vessel abnormality. The conjunctiva, cornea, and retina were affected together in 5 hemizygous patients (33.3%). One hemizygous patient had all FDrelated ocular manifestations in both eyes. Conclusion: To our knowledge, this study is the first to describe the ocular manifestations of FD in the Turkish population. Although cornea verticillata is considered a hallmark of FD, it was absent in approximately one-third of patients. Moreover, cataract, another well-known feature of FD, was present in only 26.6% of the patients. Conjunctival vascular abnormality alone seems to be quite rare in FD, although it often accompanies other ocular manifestations. Therefore, recognition of other mild findings and special consideration of their associations may increase the diagnostic value of ocular findings in FD.

Keywords

Author Keywords

Cornea verticillata conjunctival aneurysm Fabry cataract Fabry disease metabolic diseases

Development, optimization and validation of LC-MS/MS method for the determination of DBS GALT enzyme activity

By

Topbas, M (Topbas, Muhammet) [1] ; Canbay, E (Canbay, Erhan) [1] ; Sezer, E (Sezer, Ebru) [1] ; Canda, E (Canda, Ebru) [2] ; Ucar, SK (Ucar, Sema Kalkan) [2] ; Coker, M (Coker, Mahmut) [2] ; Sözmen, EY (Sozmen, Eser Yildirim) [1]

 (provided by Clarivate) 

Source

ANALYTICAL BIOCHEMISTRY

Volume

678

DOI

10.1016/j.ab.2023.115284

Article Number

115284

Published

OCT 1 2023

Early Access

AUG 2023

Indexed

2023-10-16

Document Type

Article

Jump to

Abstract

Galactosemia is a carbohydrate metabolism disorder often caused by galactose-1-phosphate uridyl transferase (GALT) deficiency. Detecting GALT deficiency involves measuring intra-erythrocyte enzyme activity. We aimed to create a robust liquid chromatography-mass spectrometry (LC-MS/MS) method to assess GALT activity in dried blood spot (DBS) samples. We validated this method and compared it to the fluorometric approach. We investigated the impact of K2EDTA and lithium heparin tubes on enzyme activity to identify the best sample collection tube. We also assessed the reaction-stopping method. The developed approach employed [13C6]galactose-1-phosphate as a substrate and UDP-N-acetylglycosamine as an internal standard (IS). The mean +/- SD value for GALT activity of DBS samples was determined as 6.37 +/- 1.96 mu mol/gHb/hour. The linear range was 0.4-50 mu M (2.4-310% of normal) in the DBS method. The % coefficient of variation (%CV) values were less than 15 for intra-day and inter-day repeatability studies. Over 90% recovery was achieved in recovery studies, and no ion suppression from matrix was detected. DBS samples were quite stable for 31 days under different storage conditions. Enzyme activity results reported as <3.5 U/g Hb by fluorometric method, were quantitatively determined for even very low concentrations by LC-MS/MS method.

Keywords

Author Keywords

GALTLC-MS/MS Newborn screening Dried blood spot

Assessment of Histamine and Diamine Oxidase Levels in Patients with Hashimoto's Thyroiditis: A Cross-Sectional Study

By

Ozisik, H (Ozisik, Hatice) [1] ; Yelpaze, M (Yelpaze, Mehmet) [2] ; Seren, N (Seren, Nazlican) [3] ; Canbay, E (Canbay, Erhan) [3] ; Ulas, E (Ulas, Ezgi) [1] ; Kocabas, GÜ (Kocabas, Gokcen Unal) [1] ; Sözmen, EY (Sozmen, Eser Yildirim) [3] ; Erdogan, M (Erdogan, Mehmet) [1] ; Yürekli, BS (Yurekli, Banu Sarer) [1]

 (provided by Clarivate) 

Source

ENDOCRINOLOGY RESEARCH AND PRACTICE

Volume

29

Issue

3

DOI

10.5152/erp.2025.24588

Published

JUL 2025

Indexed

2025-08-03

Document Type

Article

Abstract

Objective: Chronic inflammation in Hashimoto's thyroiditis (HT) might influence histamine metabolism and DAO activity and limited data exists about this condition. Methods: Data were obtained from 25 HT patients and 38 healthy controls who visited the outpatient clinic between December 2023 and March 2024. Demographic, clinical, and laboratory information were extracted from patient records. Thyroid-stimulating hormone, free T4, free triiodothyronine, anti-TPO, and anti-thyroglobulin antibodies levels were retrieved from the files, while histamine, serotonin and tryptophan levels were detected using the LC-MS/MS method. Results:The mean ages of HT and control groups were 40.52 +/- 11.60 and 36.61 +/- 11.04 years, respectively. Notable statistical differences were identified among the groups concerning anti-thyroglobulin antibodies, anti-thyroid peroxidase antibodies, high-density lipoprotein, and triglyceride concentrations. The levels of histamine, serotonin, and tryptophan were not statistically different between the 2 groups, however, a statistically significant elevation in DAO levels was assessed in the HT group relative to the control group. Conclusion: The data figured out borderline significant changes in DAO levels in the serum of HT compared to control group, and serum levels of DAO were higher in HT.This is the initial investigation to assess DAO levels in HT and compared histamine and DAO levels in HT.

Keywords

Author Keywords

Diamine oxidase Hashimoto's thyroiditis histamine thyroid

Solvation Methods Affect the Amount of Active Components in the Extract of Propolis as well as Its Anti-Inflammatory Activity in THP-1 Cells

By

Durmaz, B (Durmaz, Burak) [1] ; Celebi, LMO (Celebi, Latife Merve Oktay) [2] ; Memmedov, H (Memmedov, Hikmet) [3] ; Gunel, NS (Gunel, Nur Selvi) [2] ; Yildirim, HK (Yildirim, Hatice Kalkan) [4] ; Sozmen, EY (Sozmen, Eser Yildirim) [1]

 (provided by Clarivate) 

Source

EUROPEAN JOURNAL OF BIOLOGY

Volume

82

Issue

2

Page

132-141

DOI

10.26650/EurJBiol.2023.1247199

Published

DEC 2023

Indexed

2023-12-01

Document Type

Article

Abstract

Objective: : Propolis has been found to have various effects, including antioxidant and anti-inflammatory properties, according to studies. In this recent research, we discovered that reducing allergenic compounds in propolis through biotransformation using specific Lactobacillus plantarum strains enhanced its anti-inflammatory qualities. The study aimed to identify the extraction methods and solvents that had the most significant anti-inflammatory effects and assess how L. plantarum strains biotransformation of propolis affected these qualities in THP-1 cell line cultures.

Materials and Methods: Propolis samples were biotransformed with different concentrations (1.5%, 2.5%, 3.5%) of several L. plantarum strains (ISLG-2, ATCC (R) 8014, visbyvac) before extraction using various solvents (ethanol, polyethylene glycol-PEG, water) and ultrasound treatments (300 W/40 Hz for 5, 10, 15 min). Liquid chromatography-mass spectrometer/mass spectrometry was used for phenolic analysis of the samples. ELISA test kits were employed to assess NF-k beta, IL-1 alpha, IL-1 beta, IL-6, IL-10, TNF-alpha, IFN-gamma, COX-1 in the cell culture supernatant.

Results: : Results showed that, except for NF-k beta, all cytokine levels decreased in four separate propolis samples. Caffeic acid, kaempferol, ferulic acid, quercetin, pelargonin, and naringenin were the key physiologically active components associated with the anti-inflammatory activity of propolis. The biotransformation process to reduce allergen compounds did not alter propolis's anti-inflammatory properties.

Conclusion: In samples that were dissolved in water, dissolved in ethanol+biotransformed with L. plantarum ATCC (R) 8014, dissolved in water+biotransformed with L. plantarum ATCC (R) 8014, and dissolved in water+sonicated for 15 min and biotransformed with L. plantarum ATCC (R) 8014, the maximum anti-inflammatory effect of propolis was assessed.

Keywords

Author Keywords

LPS inflammation propolis biotransformation extraction

Glycosaminoglycan-induced proinflammatory cytokine levels as disease marker in mucopolysaccharidosis

By

Copur, O (Copur, Oznur) [1] , [2] ; Yazici, H (Yazici, Havva) [3] ; Canbay, E (Canbay, Erhan) [2] ; Durmaz, B (Durmaz, Burak) [1] , [2] ; Canda, E (Canda, Ebru) [3] ; Ucar, SK (Ucar, Sema Kalkan) [3] ; Coker, M (Coker, Mahmut) [3] ; Sozmen, EY (Sozmen, Eser Yildirim) [2]

 (provided by Clarivate) 

Source

CYTOKINE

Volume

173

DOI

10.1016/j.cyto.2023.156410

Article Number

156410

Published

JAN 2024

Early Access

NOV 2023

Indexed

2023-12-12

Document Type

Article

Jump to

Abstract

Recently, it has been shown disturbances in oxidant/antioxidant system and increases in some inflammatory markers in animal studies and in some Mucopolysaccharidoses (MPSs) patients. In this study, we aimed to determine the oxidative stress/antioxidant parameters and pro-inflammatory cytokine levels in the serum of MPS patients, in order to evaluate the possible role of inflammation in these patient groups regarding to accumulated metabolites. MPS I (n = 3), MPS II (n = 8), MPS III (n = 4), MPS IVA (n = 3), MPS VI (n = 3), and VII (n = 1) patients and 20 age-matched healthy subjects were included into the study. There was no statistically significant change in activities of SOD, Catalase, GSH-Px and lipid peroxidation levels in erythrocytes between the MPS patients and healthy controls. While IL-1alpha (p = 0.054), IL-6 (p = 0.008) levels, and chitotriosidase activity (p = 0.003) elevated in MPS3 patients, IL1 alpha (p = 0.006), IL-1 beta (p = 0.006), IL-6 (p = 0.006), IFN gamma (p = 0.006), and NFxB (p = 0.006) levels increased in MPS-6 patients. Elevated levels of IL-6, IL1 alpha and chitotriosidase activity demonstrated macrophage activation in MPSIII untreated with enzyme replacement. Our study showed for the first time that high levels of IL1 alpha, IL-6, IL1 beta and NFxB were present in MPSVI patients, demonstrating the induction of inflammation by dermatan sulphate. The low level of paraoxonase in MPSVI patients may be a good marker for cardiac involvement. Overall, this study provides important insights into the relationship between lysosomal storage of glycosaminoglycan and inflammation in MPS patients. It highlights possible pathways for the increased release of inflammatory molecules and suggests new targets for the development of treatments.

Keywords

Author Keywords

Mucopolysaccharidosis Paraoxonase Inflammation

Liver Histology and Biochemistry of Exposed Newborn and Infant Rats with Experimental Aflatoxicosis

By

Kiliç, KD (Kilic, Kubilay Dogan) [1] ; Gökhan, A (Gokhan, Aylin) [1] ; Sözmen, EY (Sozmen, Eser Yildirim) [2] ; Uysal, A (Uysal, Aysegul) [1]

 (provided by Clarivate) 

Source

PAKISTAN VETERINARY JOURNAL

Volume

42

Issue

4

Page

453-460

DOI

10.29261/pakvetj/2022.066

Published

2022

Indexed

2022-12-21

Document Type

Article

Abstract

Gestational and lactational transmission of Aflatoxin B1 (AFB1) can elicit several toxic effects emphasizing the severity of aflatoxicosis. The present study aimed to investigate the genotoxic effects of prenatal and postnatal exposure to AFB1 on the livers of exposed offspring. With this aim, 50 mu g/kg/body weight per day AFB1 was administered intraperitoneally (i.p.) to pregnant and lactating dam rats. Pups grouped as newborns (GD21/PND0) exposed in utero and infants exposed through breast milk (PND21) were compared with body weight measurements. Liver tissues were weighed after removal and subjected to histochemical (HC), immunohistochemical (IHC) and biochemical analyzes. The body weight and liver weight of exposed newborns were significantly lower than control (P<0.05). The histomorphological changes were more pronounced in exposed newborns. A decrease (P<0.05) in the histological score (HSCORE) of cytokeratin 19 (CK19) IHC, fetal stem/progenitor cells marker, and an increase (P<0.05) in the HSCORE of alpha-fetoprotein (AFP) IHC, hepatocellular carcinoma (HCC) marker, were detected in both exposed groups. Exposed newborns showed higher CK19 and AFP HSCORE than exposed infants (P<0.05). Both groups exhibited a low proliferation index score of proliferating cell nuclear antigen (PCNA) IHC (P<0.05). The high apoptotic index score of immunofluorescence (IF) staining of the terminal deoxytransferase-mediated dUTP nick-end labeling (TUNEL) method was significant in exposed newborns (P<0.05). Evaluation of oxidative stress and antioxidant systems revealed that tissue malondialdehyde (MDA) levels decreased in exposed newborns and increased in exposed infants (P<0.05), and tissue catalase (CAT) levels increased in both groups (P<0.05). In conclusion, the effects of AFB1 exposure during the gestational period occurred more severely, and the importance of preventing AFB1 exposure was revealed.

Keywords

Author Keywords

Gestation Lactation Infant Rats Liver Food safety Newborn Aflatoxin

The effectiveness of interactive training and microlearning approaches on motivation and independent learning of medical students during the COVID-19 pandemic

By

Sözmen, EY (Sozmen, Eser Yildirim) [1] , [2] ; Karaca, O (Karaca, Ozan) [3] ; Bati, AH (Bati, A. Hilal) [3]

 (provided by Clarivate) 

Source

INNOVATIONS IN EDUCATION AND TEACHING INTERNATIONAL

Volume

60

Issue

1

Page

70-79

DOI

10.1080/14703297.2021.1966488

Published

JAN 2 2023

Early Access

AUG 2021

Indexed

2021-08-18

Document Type

Article

Jump to

Abstract

In the COVID-19 pandemic period, most students felt lonely, accordingly their motivation for self-learning was low. The study aimed to increase their enthusiasm for work, and to improve their learning of biochemical subjects in the medical education programme during the pandemic period. Small parts of educational materials (microlearning) were presented in different formats such as videos, interactive education exercises, game-based quizzes and final exam was taken. The achievement of learning objectives of the course was evaluated by a survey. Students participating in daily activities gained higher self-confidence in learning and their final exam grade was higher compared to non-participating students (7.35 +/- 1.37 vs 7.06 +/- 1.88, p = 0.038). Most of the students agreed that daily activities (52%) increased their motivation to work. This approach might be proposed as a practice that supports distance learning, or even traditional education, especially in medical education that includes complex issues.

Keywords

Author Keywords

Microlearning distance learning self-learning COVID-19

Nasal fluid sample as a reliable matrix for determination of cytokine levels in childhood asthma

By

Doulatpanah, M (Doulatpanah, Mojtaba) [1] ; Kocamanoglu, M (Kocamanoglu, Meltem) [1] ; Sözmen, EY (Sozmen, Eser Yildirim) [1] ; Öztürk, GK (Ozturk, Gokcen Kartal) [2] ; Demir, E (Demir, Esen) [2] ; Gülen, F (Gulen, Figen) [2] ; Akçay, Y (Akcay, Yasemin) [1]

 (provided by Clarivate) 

Source

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI

Volume

48

Issue

5

Page

507-514

DOI

10.1515/tjb-2022-0147

Published

OCT 20 2023

Early Access

AUG 2023

Indexed

2023-08-25

Document Type

Article

Jump to

Abstract

Objectives: Childhood asthma is a chronic disease with high incidence worldwide. As a lifelong disease, asthma has episodes. Inflammation continues to occur in the clinical remission of asthma. It can be difficult to diagnose childhood asthma, especially in clinical remission. We hypothesized that some cytokines secreted to nasal fluid from the airway during inflammation might help diagnose clinical remission of asthma. Moreover, sampling nasal fluid is an easy and non-invasive procedure, so it may be a preferable sampling method.Methods: We measured levels of some interleukins (ILs), which are IL-4, IL-5, IL-6, IL-12p70, IL-13, IL-33, granulocyte-macrophage colony-stimulating factor (GM-CSF), periostin and thymic stromal lymphopoietin (TSLP) by Luminex magnetic bead-based immunoassay in nasal fluid and in serum of asthmatic children in clinical remission.Results: We found that IL-5, IL-6, IL-33, and periostin had elevated levels in nasal fluid. IL-5 and IL-33 had increased levels in the nasal fluid of the patients with immunoglobulin E (IgE) high and low phenotypes. While the nasal fluid TSLP levels were positively correlated with most of the increased serum cytokine levels of non-allergic asthmatic children, the nasal fluid GM-CSF levels were positively correlated with most of the increased serum cytokine levels of the allergic asthmatic children.Conclusions: IL-5, IL-6, IL-33, and periostin had elevated levels in the nasal fluid of the patients in clinical remission. The nasal fluid GM-CSF levels of the allergic patients and nasal fluid TSLP levels of the non-allergic patients had a positive correlation with most of the serum cytokine levels. Thus, our results showed that nasal fluid might be a preferable biological sample to diagnose asthma in children.

Keywords

Author Keywords

biomarker childhood asthma cytokines immune response remission

Preventive effect of nebivolol on contrast-induced nephropathy in rats

By

Toprak, O (Toprak, Omer) [1] ; Cirit, M (Cirit, Mustafa) [1] ; Tanrisev, M (Tanrisev, Mehmet) [1] ; Yazici, C (Yazici, Cevat) [2] ; Canoz, O (Canoz, Ozlem) [3] ; Sipahioglu, M (Sipahioglu, Murat) [4] ; Uzum, A (Uzum, Atilla) [1] ; Ersoy, R (Ersoy, Rifki) [1] ; Sozmen, EY (Sozmen, Eser Yildirim) [5]

 (provided by Clarivate) 

Source

NEPHROLOGY DIALYSIS TRANSPLANTATION

Volume

23

Issue

3

Page

853-859

DOI

10.1093/ndt/gfm691

Published

MAR 2008

Indexed

2008-03-01

Document Type

Article

Abstract

Background. Altered renal vasodilatation and oxidative stress are important mechanisms of contrast-induced nephropathy (CIN). The aim of the present study was to assess the effect of nebivolol, a beta blocker, on prevention of CIN. We hypothesized that nebivolol may prevent CIN due to its renal vasodilatation and antioxidant effects.

Methods. Thirty-two Wistar-albino rats were divided into four groups (n = 8 each): control (C), contrast media (CM), nebivolol (N), and nebivolol + contrast media (NCM). CIN was induced by administration of intravenous high-osmolar contrast media diatrizoate (6 ml/kg) after 72 h of dehydration. Nebivolol (2 mg/kg) was given internally once daily for 5 days. Kidney function parameters, nitric oxide metabolites and oxidative stress markers were measured. Kidneys were excised for pathological evaluation.

Results. The decrease of creatinine clearance was 0.180 +/- 0.11 mg/dl in CM, and 0.030 +/- 0.10 mg/dl in NCM (P = 0.01). Microproteinuria was ameliorated using nebivolol (P = 0.001). Serum protein carbonyl content, malonyldialdehyde and kidney thiobarbituric acid-reacting substances levels were higher in CM than in C (P = 0.003, P < 0.001 and P = 0.034, respectively) and serum thiol was lower in CM than in C (P = 0.001). However, oxidative stress markers were similar in NCM and C. Diatrizoate decreased kidney nitrite levels, but nebivolol increased them (P = 0.027). Nebivolol attenuated the tubular necrosis, proteinaceous casts and medullary congestion, although significant protective effects, were observed in tubular necrosis (P = 0.001) and proteinaceous cast (P < 0.001).

Conclusion. This study demonstrated the protective role of nebivolol against CIN.

Keywords

Author Keywords

contrast-induced nephropathy contrast media nebivolol nitric oxide oxidative stress

A critical review on human serum Paraoxonase-1 in the literature: truths and misconceptions

By

Mackness, M (Mackness, Michael) [1] ; Sozmen, EY (Sozmen, Eser Yildirim) [2]

 (provided by Clarivate) 

Source

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI

Volume

46

Issue

1

Page

1-6

DOI

10.1515/tjb-2020-0186

Published

FEB 2021

Indexed

2021-03-26

Document Type

Review

Abstract

Human serum paraoxonase 1 (PON1) appears to play an important role in the development of a large variety of diseases with an inflammatory component including heart disease, diabetes, rheumatic diseases, neurological diseases and cancer. As such PON1 research is rapidly expanding into new biomedical fields. Unfortunately, this rapid expansion has resulted in a number of problems due to poor experimental design and the spreading of misconceptions in the literature. This review seeks to describe the basic properties of PON1 and the problems and misconceptions that have arisen.

Keywords

Author Keywords

hastalik lactonase paraoxonase polymorphisms

High chitotriosidase and AGE levels in acromegaly: a case-control study

By

Ozisik, H (Ozisik, Hatice) [1] ; Yurekli, BS (Yurekli, Banu Sarer) [1] ; Suner, A (Suner, Asli) [2] ; Copur, O (Copur, Oznur) [3] ; Sozmen, EY (Sozmen, Eser Yildirim) [3] ; Ozbek, SS (Ozbek, Suha Sureyya) [4] ; Karabulut, AK (Karabulut, Ahmet Kasim) [4] ; Simsir, IY (Simsir, Ilgin Yildirim) [1] ; Erdogan, M (Erdogan, Mehmet) [1] ; Cetinkalp, S (Cetinkalp, Sevki) [1] ; 

 (provided by Clarivate) 

Source

HORMONES-INTERNATIONAL JOURNAL OF ENDOCRINOLOGY AND METABOLISM

Volume

22

Issue

1

Page

61-69

DOI

10.1007/s42000-022-00409-3

Published

MAR 2023

Early Access

OCT 2022

Indexed

2022-11-04

Document Type

Article

Jump to

Abstract

Purpose Acromegaly is associated with oxidative stress and inflammation parameters. Chitotriosidase (CHITO) is a marker of macrophage activation and plays a pivotal role in the activation of inflammatory and immunological responses. Our study aimed to determine CHITO,YKL-40, advanced glycation end product (AGE), and high-sensitivity C-reactive protein (hsCRP) levels to investigate malondialdehyde (MDA), catalase, superoxide dismutase (SOD), and glutathione peroxidase (GSH-Px) activities and to evaluate any association of these parameters with carotid intima media thickness (cIMT) in patients with controlled acromegaly. Methods Thirty controlled acromegaly patients and 41 age- and sex-matched control cases were studied. We obtained demographic data, hormonal and metabolic parameters, and cIMT. CHITO activity was measured with the fluorometric method of Chamoles et al. YKL-40 and hsCRP levels were measured using ELISA. AGEs were measured based on spectrofluorimetric detection. GSH-Px activity was determined by a colorimetric assay. MDA, SOD, and catalase activities were determined in hemolysis. Results Higher CHITO, AGE, and hsCRP concentrations were observed in patients with acromegaly compared to controls. SOD levels were non-significantly higher in the acromegaly group, while catalase activities were lower in patients with acromegaly. Correlation analyses of CHITO, AGEs, YKL-40, hsCRP, MDA, catalase, GSH-Px, and SOD with metabolic, anthropometric, and laboratory parameters did not demonstrate any significant correlation (p > 0.05). There was no significant difference between groups with regard to cIMT levels. Conclusion This is the first study investigating CHITO and AGE levels in patients with acromegaly. Serum CHITO, AGE, and hsCRP levels in acromegalic patients were significantly increased. It may be important to evaluate CHITO, AGE, and hsCRP levels in acromegalic patients who are already under cardiometabolic surveillance due to risk of developing cardiovascular disease.

Keywords

Author Keywords

Acromegaly ChitotriosidaseYKL-40Advanced glycation end products High-sensitivity CRP Malondialdehyde

The effect of temperature and light intensity during cultivation of Chlorella miniata on antioxidant, anti-inflammatory potentials and phenolic compound accumulation

By

Sozmen, AB (Sozmen, Alper Baran) [1] ; Canbay, E (Canbay, Erhan) [2] ; Sozmen, EY (Sozmen, Eser Yildirim) [2] ; Ovez, B (Ovez, Bikem) [1]

 (provided by Clarivate) 

Source

BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY

Volume

14

Page

366-374

DOI

10.1016/j.bcab.2018.03.023

Published

APR 2018

Indexed

2018-06-05

Document Type

Article

Abstract

The purpose of this study was to see if the microalgae species Chlorella miniata possess an antioxidant and antiinflammatorypotential and to see if there is a correlation between the cultivation conditions and these potentials. For this purpose, cultivation of the algae under different growth conditions had been carried out; afterwards the soxhletextraction of the produced biomass had been completed. Lastly the assays of Trolox equivalent antioxidant capacity (TEAC), ferric reducing antioxidant capacity (FARP), total antioxidant capacity (TAO), xanthine oxidase inhibition, and hyaluronidase inhibition were studied using extracts of microalgae obtained from the different cultivation parameters. Ifs important to mention that this was the first study using these assays on species of Chlorella miniata and the first that utilized the cultivation parameters on microalgae. The results were, 22,91 mg/g algae TEAC, 776,70 mg/g algae gallic acid equivalent of FRAP and 37,07% xanthine oxidase inhibition with a concentration of 10,5 mg algae per ml ethanol, 65,39% of hyaluronidase inhibition with a concentration of 0,9 mg algae per ml ethanol. The effects of the cultivation parameters on these assays and the Lc MS/MS analyses for various phenolic compounds in the extracts were also a part of the study.

Keywords

Author Keywords

Chlorella miniata Soxhlet extraction LC MS/MS Anti-oxidant potential Antiinflammatory

Propolis prevents inhibition of apoptosis by potassium bromate in CCD 841 human colon cell

By

Memmedov, H (Memmedov, Hikmet) [1] ; Oktay, LM (Oktay, Latife Merve) [2] ; Durmaz, B (Durmaz, Burak) [1] ; Günel, NS (Gunel, Nur Selvi) [2] ; Yildirim, HK (Yildirim, Hatice Kalkan) [3] ; Sözmen, EY (Sozmen, Eser Yildirim) [1]

 (provided by Clarivate) 

Source

CELL BIOCHEMISTRY AND FUNCTION

Volume

38

Issue

4

Page

510-519

DOI

10.1002/cbf.3475

Published

JUN 2020

Early Access

JAN 2020

Indexed

2020-02-06

Document Type

Article

Abstract

Previously, we demonstrated that biotransformation of propolis by some special strains of Lactobacillus plantarum might decrease the allergenic molecules in propolis. In this study, we aimed to investigate the effect of biotransformation of propolis on its antioxidant effect and its protective effect against potassium bromate-induced cancer in human colon cell line. Propolis samples were treated with different solutions (ethanol, polyethylene glycol, and water), and ultrasonication was applied at 40 Hz (5, 10, and 15 minutes) in order to facilitate solvation of solid samples. Fermentations were performed by L. plantarum strains (ISLG-2, ATCC-8014, and Visbyvac). The phenolic content of propolis was determined with liquid chromatography-mass spectrometry/mass spectrometry (LCMS/MS). The antioxidant activity (antioxidant enzymes, lipid peroxidation) and apoptosis markers (caspase 3,8,9, cytochrome-c, tumour necrosis factor-related apoptosis-inducing ligand-R1 and R2 [TRAIL], and apoptosis protease activating factor-1 [APAF-1] levels) were determined in CCD 841-human colon cell line after induction of oxidative stress by potassium bromate. All propolis samples in different solvents induced apoptosis and 4 biotransformed (by L. plantarum ISL-2 strain and L. plantarum ATCC 8014 strain) propolis samples with low allergenic molecules demonstrated similar inductions of apoptosis in CCD841 cell line. In conclusion, reduction of allergenic molecules in propolis via biotransformation did not change the antioxidant and protective effects of propolis, and it is suggested as a potential therapeutic molecule in prevention of colon cancer caused by oxidative stress for all patients. Significance of the study This study is the first investigation that shows protective effect of propolis against potassium bromate toxicity by means of decreasing lipid peroxidation and reversing the main molecule levels in intrinsic and extrinsic pathway of apoptosis. Biotransformed propolis samples by L. plantarum ISL-2 and ATCC 8014 strain with low allergen molecule content has also the same effect in potassium bromate toxicity in CCD841 colon cell. Our data contributed that propolis as a natural compound might be a good candidate due to its minimal toxicity and lack of any adverse effects to prevent carcinogenic effect of potassium bromate.

Keywords

Author Keywords

apoptosis potassium bromate propolis

Palosuran in Gentamicin-Induced Acute Kidney Injury in an Experimental Rat Model

By

Burgucu, HC (Burgucu, Hatice Caliskan) [1] ; Olukman, M (Olukman, Murat) [2] ; Coskunsever, D (Coskunsever, Deniz) [2] ; Sen, S (Sen, Sait) [3] ; Sözmen, EY (Sozmen, Eser Yildirim) [4] ; Can, M (Can, Mustafa) [1] ; Duman, S (Duman, Soner) [5]

 (provided by Clarivate) 

Source

TURKISH JOURNAL OF NEPHROLOGY

Volume

31

Issue

2

Page

127-133

DOI

10.5152/turkjnephrol.2022.21071

Published

APR 2022

Indexed

2022-08-23

Document Type

Article

Abstract

Objective: Urotensin-II is a potent vasoconstrictor peptide and has fibrotic effects in the heart and kidneys and palosuran is a selective urotensin-II receptor antagonist. This study aimed to investigate the effects of palosuran, which has antifibrotic effects, in an acute kidney injury model formed with gentamicin and to reveal new treatment options for acute kidney injury cases.

Methods: A total of 24 Wistar albino rats were randomly divided into 3 groups of 8 animals as the control group, the gentamicin group, and the gentamicin+palosuran group. The rats in the control group received a 1 x 1 mL intramuscular injection of saline for 8 days. To create acute kidney injury in the rats in the gentamicin group, an intramuscular injection of 100 mg/kg/day gentamycine was applied for 8 days. In the gentamicin + palosuran group, an intramuscular injection of 100 mg/kg/day gentamycine was applied for 8 days, together with 300 mg/kg palosuran dissolved in distilled water and administered by oral gavage twice a day for the same period. Serum urea, creatinine, calcium, albumin, total protein levels, and urine gamma-glutamyl transferase and protein levels were evaluated for all the groups. In addition, tubular degeneration, tubular necrosis, tubular regeneration, tubulointerstitial nephritis, microcalcification, and total histological scores of these groups were also examined histopathologically.

Results: The urine gamma-glutamyl transferase levels of the gentamicin and gentamicin + palosuran groups were significantly higher than those of the control group (311 +/- 60.5 and 140 +/- 39.5 vs. 0.33 +/- 0.22, P <.05). On the eighth day, the gamma-glutamyl transferase levels of the gentamicin + palosuran group were significantly lower than the gentamicin group (311 +/- 60.5 vs. 140 +/- 39.5, P <.05). A significant decrease was obtained in tubular necrosis in the gentamicin + palosuran group compared to the gentamicin group (2.5 +/- 0.3 vs. 1.3 +/- 0.4, P <.05). A statistically significant decrease was determined in the total histological score in the gentamicin + palosuran group compared to the gentamicin group (6.4 +/- 0.7 vs. 4.7 +/- 0.7, P <.05). Our results showed that the urotensin-II receptor antagonist, palosuran, had positive effects on tubular necrosis, tubular degeneration, tubular regeneration, and the total histological score in an experimental acute kidney injury model formed with gentamicin.

Conclusion: The study results suggest that urotensin-II may play a role in the physiopathology of acute renal failure associated with gentamicin. These results are promising for the use of palosuran in the treatment of acute kidney injury.

Keywords

Author Keywords

Acute kidney injury urotensin-II palosuran gentamicin

The decision-making levels of urine tetrasaccharide for the diagnosis of Pompe disease in the Turkish population

By

Canbay, E (Canbay, Erhan) [1] ; Vural, M (Vural, Melisa) [1] ; Uçar, SK (Ucar, Sema Kalkan) [2] ; Sezer, ED (Sezer, Ebru Demirel) [1] ; Karasoy, H (Karasoy, Hatice) [3] ; Yüceyar, AN (Yuceyar, Ayse Nur) [3] ; Çoker, M (Coker, Mahmut) [2] ; Sözmen, EY (Sozmen, Eser Yildirim) [1]

 (provided by Clarivate) 

Source

JOURNAL OF PEDIATRIC ENDOCRINOLOGY & METABOLISM

Volume

33

Issue

3

Page

391-395

DOI

10.1515/jpem-2019-0393

Published

MAR 2020

Indexed

2020-03-24

Document Type

Article

Abstract

Background: Recently, urinary excretion of the tetrasaccharide 6-alpha-D-glucopyranosyl-maltotriose (Glc4) has been proposed as a marker for the diagnosis and monitoring of Pompe disease (PD). We aimed to determine the reference intervals and reliable decision-making levels of urine tetrasaccharide concentrations for the diagnosis of infantileand late-onset Pompe patients in the Turkish population.

Methods: In this study, nine patients with PD (five of them with late-onset PD [LOPD]) and 226 healthy individuals (aged 0-64 years) were included. Urine Glc4 concentrations were determined using the ultra-high-performance liquid chromatography (UHPLC) tandem mass spectrometry (MS/MS) method.

Results: Our data showed that the urine tetrasaccharide levels decreased with age in healthy individuals (p < 0.001, r = -0.256). It was higher especially during the first year of life compared to that in the elder subjects. The tetrasaccharide level of Pompe patients was higher compared to that of healthy controls of the same age: 99 +/- 68 mmol/mol creatinine for infantile onset vs. 4.0 +/- 3.0 mmol/mol creatinine for healthy controls of the same age group and 12.1 +/- 17.4 mmol/mol creatinine for late onset vs. 1.7 +/- 1.2 mmol/mol creatinine for healthy controls of the same age group.

Conclusions: The results of this study showed that the reference intervals of tetrasaccharide in urine changed over time; therefore, it is critically important to define agebased decision levels for the diagnosis of LOPD.

Keywords

Author Keywords

Pompe disease reference intervals tetrasaccharide 6-alpha-D-glucopyranosyl-maltotriose

A novel marker of systemic inflammation in psoriasis and related comorbidities: chitotriosidase

By

Ilanbey, B (Ilanbey, Bilal) [1] ; Elmas, ÖF (Elmas, Omer Faruk) [2] ; Sözmen, EY (Sozmen, Eser Yildirim) [3] ; Günay, Ü (Gunay, Umran) [4] ; Demirbas, A (Demirbas, Abdullah) [5] ; Atasoy, M (Atasoy, Mustafa) [6] ; Türsen, Ü (Tursen, Umit) [7] ; Lotti, T (Lotti, Torello) [8]

 (provided by Clarivate) 

Source

TURKISH JOURNAL OF MEDICAL SCIENCES

Volume

51

Issue

5

Page

2318-2323

DOI

10.3906/sag-2101-137

Published

2021

Indexed

2021-11-08

Document Type

Article

Abstract

Background/aim: Chitotriosidase (ChT) is an enzyme secreted by activated macrophages and neutrophils in response to proinflammatory signals. There is growing evidence indicating that ChT activity reflects the systemic inflammatory status. This study aimed to investigate whether serum ChT activity increased in patients with psoriasis and related comorbidities. Materials and methods: This cross-sectional study included 53 (28 with associated comorbidities and 25 without comorbidities) patients with psoriasis and 52 healthy volunteers. All participants underwent laboratory investigations for serum ChT levels, complete blood count, erythrocyte sedimentation rate, C-reactive protein, and serum lipid levels. Results: The patients with psoriasis showed significantly higher levels of ChT activity as compared to the healthy controls (23.5 +/- 11.4 vs. 17.5 +/- 10.4 mu mol/mL/hour; p = 0.015). Additionally, the ChT activity was significantly higher in patients with comorbidities than in those without (p = 0.042). Conclusion: Our data support the pathogenetic role of inflammatory processes induced by macrophage activation in patients with psoriasis and related comorbidities. We believe that high ChT activity in patients with psoriasis may serve as an early prediction of the possible related comorbidities.

Keywords

Author Keywords

Chitotriosidase comorbidity macrophage psoriasis

Antiproliferative effect of rosehip tea phenolics in prostate cancer cell lines

By

Özgönül, AM (Ozgonul, Ali Mert) [1] ; Asik, A (Asik, Aycan) [2] ; Durmaz, B (Durmaz, Burak) [1] ; Aslaminabad, R (Aslaminabad, Ramin) [1] ; Gündüz, C (Gunduz, Cumhur) [2] ; Sozmen, EY (Sozmen, Eser Yildirim) [1]

 (provided by Clarivate) 

Source

TURKISH JOURNAL OF BIOCHEMISTRY-TURK BIYOKIMYA DERGISI

Volume

45

Issue

4

Page

423-428

DOI

10.1515/tjb-2019-0262

Published

AUG 2020

Indexed

2020-12-03

Document Type

Article

Abstract

Objectives: Recently, phenolic compounds (quercetin, kaempferol, ellagic acid (EA), and myricetin) as natural sources have been suggested to be used for treatment and chemoprevention of prostate cancer. Since rosehip includes the above molecules in high concentration, we set out to investigate possible anti-proliferative effect of rosehip tea on the prostate cancer cell line.

Methods: The flavonol content of rosehip tea prepared at different temperatures and time intervals was determined first and then the antiproliferative effect of tea samples was established by adding tea samples to the prostate cancer cell line (VCaP and LNCaP).

Results: Quercetin was more effective in LNCaP cell than in VCaP cell (IC50 = 20 and 200 mu M, respectively). The boiled fruit shredded at minute 7 showed the highest levels of quercetin, EA and kaempferol and the boiled fruit at minute 7 had the highest levels of kaempferol and EA. The tea samples were prepared in concentrations relevant to their IC50 values, added to the VCaP and LNCaP cell lines. The antiproliferative effect of rosehip tea on VCaP cells was slightly greater than that of LNCaP cells.

Conclusion: Each of the flavonols exhibits an antiproliferative effect. Our data clearly indicated that rosehip as a natural source of all flavonols had an antiproliferative effect on androgen-sensitive prostate cancer. Now that it is important to use natural sources in cancer, rosehip seems to be a promising natural product to be used to treat the prostate illness.

Keywords

Author Keywords

ellagic acid kaempferol myricetin prostate cancer quercetin rosehip

Antioxidant and anti-inflammatory effects of biotechnologically transformed propolis

By

Aldemir, O (Aldemir, Ozan) [1] ; Yildirim, HK (Yildirim, Hatice Kalkan) [2] ; Sözmen, EY (Sozmen, Eser Yildirim) [1]

 (provided by Clarivate) 

Source

JOURNAL OF FOOD PROCESSING AND PRESERVATION

Volume

42

Issue

6

DOI

10.1111/jfpp.13642

Article Number

e13642

Published

JUN 2018

Indexed

2018-06-28

Document Type

Article

Abstract

This study investigated the effect of biotransformation on anti-inflammatory activities of propolis. Propolis was dissolved with 10% ethanol and 40% polyethyleneglycol and applied ultrasonic treatment followed by Lactobacillus plantarum fermentation at 30 degrees C/48 hrs under constant agitation conditions. The in vitro anti-inflammatory activity was determined by measuring hyaluronidase and xanthine oxidase activity. Usage of PEG as a solvent and bioconversion by 8014 strain yielded high phenolic content, antioxidant, and anti-inflammatory activity. Results indicated that the anti-inflammatory activities of propolis samples are highly related to extraction methods. Optimization of extraction and biotransformation methods are subjects of further research.

Practical applicationsIn our work, we have developed a new bio-product with reduced allergenicity by biotransformation from different Lactobacillus plantarum strains (10, 8014, ATCC) and propolis extracts prepared by dissolving in different solvents (ethanol, PEG, water). Anti-inflammatory and in-vitro antioxidant effects of this bio-product were investigated. As a result, it was found that in order to obtain a bio-product with high phenolic content and low allergic effect the most suitable bacterial strain was L. plantarum 8014 and the most suitable solvent was PEG. The most important factor limiting the use of propolis in humans is due to the allergenic components in the structure. It is thought that this new bio-product, which has low allergic effect and high antioxidant activity obtained by biotransformation of propolis, may be a new light of hope for people who can not use propolis due to allergic reasons. In the future, this bio-product is thought to provide significant benefits to human health.

Keywords

Keywords Plus

BIOLOGICAL-PROPERTIES EXTRACTS PLASMA

Acute hypoxia exposure following prenatal stress impairs hippocampus and novelty-seeking behavior in adolescent rats

By

Çelik, K (Celik, Kubra) [1] , [2] ; Bilim, P (Bilim, Petek) [1] , [3] ; Garip, G (Garip, Gurur) [4] ; Durmaz, B (Durmaz, Burak) [5] ; Sözmen, EY (Sozmen, Eser Yildirim) [5] ; Baka, M (Baka, Meral) [4]

 (provided by Clarivate) 

Source

INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE

Volume

82

Issue

1

Page

85-95

DOI

10.1002/jdn.10162

Published

FEB 2022

Early Access

DEC 2021

Indexed

2021-12-28

Document Type

Article

Jump to

Abstract

Objectives The present study aimed to investigate the effects of acute hypoxia exposure following prenatal stress on the novelty-seeking behavior and hippocampus of adolescent rats. Methods The offspring were divided into prenatal stress (PS) and non-stress (NS) groups. Both groups were exposed to hypoxia on postnatal day 10 (P10) while control groups were undisturbed. Novel object recognition task was performed in each group. Next, brains were collected to examine hippocampus via immunohistochemical and biochemical studies on postnatal day 35 (P35). Results PS decreased novelty discrimination and synaptophysin (SYN) expressions in both CA1 and CA3 of the hypoxia group prominently (p < 0.05). Nestin-expressing cells were reduced while vascular endothelial growth factor (VEGF) expression was enhanced in the subgranular zone (SGZ) of PS-hypoxia group (p < 0.05). VEGF enhancement triggered angiogenesis in the CA1 and CA3 significantly (p < 0.05). PS also increased thiobarbituric acid reactive substances (TBARS) levels in the hypoxia group as a result of oxidative stress (p < 0.05). Conclusion These findings demonstrated that PS exacerbates neurodevelopmental deficits in the hippocampus of acute hypoxia-induced offspring in adolescence.

Keywords

Author Keywords

hippocampus hypoxia novelty-seeking prenatal stress

The Effects of Vitamin D on Gentamicin-Induced Acute Kidney Injury in Experimental Rat Model

By

Hur, E (Hur, Ender) [1] ; Garip, A (Garip, Alev) [2] ; Camyar, A (Camyar, Asuman) [2] ; Ilgun, S (Ilgun, Sibel) [2] ; Ozisik, M (Ozisik, Melih) [2] ; Tuna, S (Tuna, Sena) [2] ; Olukman, M (Olukman, Murat) [3] ; Ozdemir, ZN (Ozdemir, Zehra Narli) [2] ; Sozmen, EY (Sozmen, Eser Yildirim) [4] ; Sen, S (Sen, Sait) [5] ; 

 (provided by Clarivate) 

Source

INTERNATIONAL JOURNAL OF ENDOCRINOLOGY

Volume

2013

DOI

10.1155/2013/313528

Article Number

313528

Published

2013

Indexed

2013-07-31

Document Type

Article

Abstract

Introduction. Acute kidney injury (AKI) pathogenesis is complex. Findings of gentamicin nephrotoxicity are seen in 30% of the AKI patients. Vitamin D has proven to be effective on renin expression, inflammatory response, oxidative stress, apoptosis, and atherosclerosis. We aimed to investigate the effect of vitamin D in an experimental rat model of gentamicin-induced AKI. Methods. Thirty nonuremic Wistar albino rats were divided into 3 groups: Control group, 1 mL saline intramuscular (im) daily; Genta group, gentamicin 100 mg/kg/day (im); Genta + vitamin D, gentamicin 100 mg/kg/day (im) in addition to 1 alpha, 25 (OH)(2)D-3 0.4 mcg/kg/day subcutaneously for 8 days. Blood pressures and 24-hour urine were measured. Blood urea and creatinine levels and urine tubular injury markers were measured. Renal histology was semiquantitatively assessed. Results. Urea, creatinine and urine neutrophil gelatinase-associated lipocalin, and kidney injury molecule-1 were all increased in Genta group indicating AKI model. Systolic blood pressure decreased, but urine volume and glutathione increased in Genta + Vit D group compared to Control group. Histological scores indicating tubular injury increased in Genta and Genta + Vit D groups. Conclusions. Vitamin D does not seem to be effective on histological findings although it has some beneficial effects via RAS system and a promising effect on antioxidant system.

Keywords

Keywords Plus

ACUTE-RENAL-FAILURE INDUCED NEPHROTOXICITY D-RECEPT OROXIDATIVE STRESSD ANALOG

Chitotriosidase as a possible marker of clinically evidenced atherosclerosis in dyslipidemic children

By

Kologlu, T (Kologlu, Turan) [2] ; Ucar, SK (Ucar, Sema Kalkan) [3] ; Levent, E (Levent, Erturk) [4] ; Akcay, YD (Akcay, Yasemin Delen) [2] ; Coker, M (Coker, Mahmut) [2] ; Sozmen, EY (Sozmen, Eser Yildirim) [1]

 (provided by Clarivate) 

Source

JOURNAL OF PEDIATRIC ENDOCRINOLOGY & METABOLISM

Volume

27

Issue

7-8

Page

701-708

DOI

10.1515/jpem-2013-0365

Published

JUL 2014

Indexed

2014-08-15

Document Type

Article

Abstract

A correlation has been clearly shown between inflammation markers and subclinical atherosclerosis markers in the early stages of atherogenesis in subjects with familial hypercholesterolemia (FH). The aim of this study was to investigate potential inflammation markers in the diagnosis of atherosclerosis in children with FH. A total of 48 dyslipidemic children and 24 healthy age-matched control subjects were taken into study. Inflammation and macrophage activation markers (hsCRP, myeloperoxidase, chitotriosidase, YKL-40, TNF-alpha, IL-6, IL-18, MMP-1 and MMP-9) and lipid parameters of all patients were measured. Carotid intima-media thickness (cIMT) and flow-mediated dilation (FMD) levels were determined. Our data suggested that clinically evidenced (by cIMT and FMD levels) atherosclerosis starts in the early ages in hypercholesterolemic children. Higher cholesterol levels strongly correlated with macrophage activation markers (ChT, YKL-40 and myeloperoxidase). ChT and YKL-40 seem to be the more predictable markers of atherosclerosis even in early ages (<6 years old) than other classical inflammation markers such as hs-CRP, IL-6 and TNF-alpha.

Keywords

Author Keywords

carotid intima-media thickness (cIMT) chitotriosidase familial hypercholesterolemia

False Positive Diagnosis of Lysosomal Storage Disease Based on Dried Blood Spot Sample; Leucocyte Number of a Challenging Factor

By

Sözmen, EY (Sozmen, Eser Yildirim) [1] ; Dondurmaci, M (Dondurmaci, Meral) [1] ; Uçar, SK (Ucar, Sema Kalkan) [2] ; Çoker, M (Coker, Mahmut) [2]

 (provided by Clarivate) 

Source

JOURNAL OF PEDIATRIC RESEARCH

Volume

5

Issue

1

Page

17-21

Supplement

S

DOI

10.4274/jpr.33042

Published

MAR 2018

Indexed

2018-07-13

Document Type

Article

Abstract

Aim: Recently dried blood spot (DBS) samples have been recommended as a screening test for Lysosomal Storage diseases. Although DBS samples have many advantages including non-invasiveness, cost and transportation, usage of these samples is limited by its high false positive rate. We aimed to investigate any possible effect of the leucocyte number on enzyme activity in dried blood samples in a retrospective study.

Materials and Methods: Data was collected from subjects (n=263) for whom hematological parameters were available in the database of Ege University Hospital. The lysosomal enzyme activity results (alpha glycosidase, glycocerebrosidase, alpha galactosidase, sphingomyelinase and galactocerebrosidase) were re-evaluated with regard to the leucocyte number. Enzyme activities were measured using fluorometric and liquid chromatography-tandem mass spectrometry methods.

Results: All enzyme activities closely correlated with the total number of leucocyte, since leucocytes are the main source of lysosomal enzymes. Glycocerebrosidase and galactocerebrosidase presented a positive correlation with the number of neutrophils and sphingomyelinase showed a positive correlation with the number of lymphocytes. When we recalculated the lysosomal enzyme activities with regard to the leucocyte number, the false positive rates for glycocerebrosidase, sphingomyelinase and alpha galactosidase decreased from 20%, 10.5% and 10.8% to 4.5%, 4.4% and 4.2%, respectively.

Conclusions: Our data indicated that the enzyme activity in dried blood samples including low leucocyte number might be found lower than reference intervals resulting in false positive diagnosis. We concluded that the calculation of enzyme activity with regard to the number of leucocytes might produce more reliable results and might be helpful in decreasing the false positive rate.

Keywords

Author Keywords

Dried blood spot leucocyte number lysosomal storage disease

Prevalence of Fabry Disease in Familial Mediterranean Fever Patients from Central Anatolia of Turkey

By

Huzmeli, C (Huzmeli, Can) [1] ; Candan, F (Candan, Ferhan) [1] ; Alaygut, D (Alaygut, Demet) [2] ; Bagci, G (Bagci, Gokhan) [3] , [4] ; Akkaya, L (Akkaya, Lale) [1] ; Bagci, B (Bagci, Binnur) [4] ; Sozmen, EY (Sozmen, Eser Yildirim) [5] ; Kurtulgan, HK (Kurtulgan, Hande Kucuk) [3] ; Kayatas, M (Kayatas, Mansur) [1]

 (provided by Clarivate) 

Source

BIOCHEMICAL GENETICS

Volume

54

Issue

4

Page

448-456

DOI

10.1007/s10528-016-9731-3

Published

AUG 2016

Indexed

2016-08-03

Document Type

Article

Abstract

Fabry disease (FD) is a progressive, X-linked inherited disorder of glycosphingolipid metabolism due to deficient or absent lysosomal alpha-galactosidase A (AGALA) activity. FD and familial Mediterranean fever (FMF) have typical clinical similarities, and both diseases may progress to end-stage renal diseases. In this study, we aimed to determine the prevalence of FD in patients with FMF from Central Anatolia of Turkey. The study group consisted of 177 FMF patients, followed up by the Adult and Pediatric Nephrology Clinic of Cumhuriyet University Hospital. Screening for AGALA activity was performed by the dry blood spot method. Mutation analysis for GLA gene was carried out for patients having an AGALA enzyme activity value lower than the normal reference value. Low AGALA activity was detected in 23 (13 %) patients. Heterozygous GLA gene mutation c.[937G > T] p.[D313Y] was detected in one female patient (0.56 %). The patient was a 53-year-old female with proteinuria and who had undergone left nephrectomy; her glomerular filtration rate (GFR) by scintigraphy was found to be 70 ml/min. She had M694V mutation and no clinical manifestation of FD. In our study, the prevalence rate of FD was found as 0.56 % in FMF patients. The similarities between the symptoms of FMF and FD might lead to a diagnostic dilemma in physicians at countries where FMF is observed frequently. Although the prevalence of FD is rare, physicians should keep in mind that FD has an ambiguous symptomology pattern of FMF.

Keywords

Author Keywords

Fabry disease FMFAlpha-galactosidase GLA MEFV D313Y

Methods for Determination of α-Glycosidase, β-Glycosidase, and α-Galactosidase Activities in Dried Blood Spot Samples

By

Sozmen, EY (Sozmen, Eser Yildirim) [1] , [2] ; Sezer, ED (Sezer, Ebru Demirel) [1] , [2]

Edited by

Ollinger, K (Ollinger, K) ; Appelqvist, H (Appelqvist, H)

 (provided by Clarivate) 

Source

LYSOSOMES

Volume

1594

Page

255-264

DOI

10.1007/978-1-4939-6934-0_17

Book Series

Methods in Molecular Biology

Published

2017

Indexed

2017-01-01

Document Type

Article; Book Chapter

Abstract

The lysosomal storage diseases (LDSs) are a heterogeneous group of inherited genetic disorders caused by defects of lysosomal proteins. The accumulation of undigested substrates from different catabolic pathways leads to cellular dysfunction. LSDs generally presents during early childhood and have a devastating impact on the families and on public health. Over the years, approaches for treatment of some LSDs have been developed with different strategies. Increasing availability of treatments of these diseases has accelerated the development of new methods and techniques for rapid diagnosis in patients with clinical indication.

The use of dried blood spot (DBS) test has been proposed as a first tier test to identify patients with Gaucher, Pompe, and Fabry diseases. DBS usage is advantageous for the purpose of screening as it is non-invasive, sensitive, has low-cost and fast turnaround time compared to measurements in leucocyte and/or fibroblast culture. This chapter focuses on the activity measurement of three lysosomal enzymes (alpha-glucosidase, beta-glucosidase, and alpha galactosidase) in DBS samples by using fluorescent substrates and by the LC-MS/MS (liquid chromatography-mass spectrometry) method. All steps of the methods, from preparation of the solutions to calculation of the enzyme activity, will be explained in detail.

Possible diagnostic markers for Mucopolysaccharidoses; Cathepsin-D, Galectin-3 and Chitotriosidase

By

Ergun, P (Ergun, Pelin) [1] ; Kagnici, M (Kagnici, Mehtap) [2] ; Uçar, SK (Ucar, Sema Kalkan) [3] ; Çoker, M (Coker, Mahmut) [3] ; Akçay, YD (Akcay, Yasemin Delen) [1] ; Sözmen, EY (Sozmen, Eser Yildirim) [1]

 (provided by Clarivate) 

Source

IZMIR DR BEHCET UZ COCUK HASTANESI DERGISI

Volume

7

Issue

2

Page

95-100

DOI

10.5222/buchd.2017.095

Published

AUG 2017

Indexed

2017-12-29

Document Type

Article

Abstract

Objective: Mucopolysaccharidoses is a group of inherited lysosomal storage diseases that are manifested by various clinical signs and symptoms (skeletal dysplasia, coarse face, progressive psychomotor retardation, cardiac and lung involvement) due to the deposition of glycosaminoglycans in tissues. Recently it has been shown that the inflammatory response to glycosaminoglycan deposition in leucocytes in animal models of mucopolysaccaridoses has increased. Here, we aimed to investigate some inflammatory markers (galectin-3, cathepsin-D and chitotriosidase) as diagnostic markers in different types of mucopolysaccharidosis and in patients with Gaucher disease and Niemann Pick A/B disease.

Methods: Plasma samples were collected from patients with mucopolysaccharidosis (n=25), Gaucher Disease (n=16), Niemann Pick A/B (n=5) and 15 healthy controls. All subjects were under the age of 18 years. Chitotriosidase enzyme activities were determined fluorometrically, cathepsin-D and galectin-3 levels were determined by using the ELISA kit.

Results: Chitotriosidase enzyme activities were statistically significantly higher in patients with MPS IV than the control group. Cathepsin-D levels were higher in patients with MPS I, MPS III and MPS IV, galectin-3 levels were higher in patients with MPS I, MPS IV and MPS VI when compared to healthy controls. All three parameters were higher in patients with Gaucher disease and Niemann Pick A/B disease.

Conclusion: Elevated levels of galectin-3 in MPS I, MPS IV and MPS VI and elevated levels of cathepsin-D in MPS I, MPS III and MPS VI support the influence of inflammatory process in the pathophysiology of mucopolysaccharidosis and might be promising new biomarkers in the diagnosis with high sensitivity and specificity.

Keywords

Author Keywords

Cathepsin-DGalectin-3chitotriosidase MPS  ELISA Mucopolysaccharidoses

BİLİMSEL VE MESLEKİ KURULUŞLARA ÜYELİKLER

EFLM-European Specialist of laboratory medicine ünvanı

Türk Biyokimya Derneği

Klinik Biyokimya Uzmanları Derneği

SSIEM-Society for the study of Inborn errors of metabolism

KURUCU OLARAK GÖREV ALDIĞI DERNEKLER

Ege Tıbbiyeliler Derneği-1999

Ege Öğretim Elemanları Derneği (EGÖDER)-2001

Tıp Eğitimini Geliştirme Derneği (TEGED)-2000

Türkiye Sürekli Eğitim Derneği-2011

ETKİNLİKLER

KATILDIĞI KURS VE EĞİTİMLER

1. Accreditation of conformity assessment bodies on biotechnology-Biobanking-ISO 20387:2018, 2021, Health and Medicine, Turkish Accreditation Agency (TURKAK)
2. Practical Laboratory Training On Diagnostics Of CLN2, MPS IVA and MPS VI- 26-28 November 2019-SANTIAGO de COMPOSTELA, Spain
3. TS EN ISO 17024 Personnel certification, 2016, Education Management and Planning, MYK
4. Laboratory. Training Course (GALNS) 30 June-4 July2014- FLORENCE, Italy
5. LAL-D DBS Assay Workshop -23-24 May 2013-YORKHİLL, Ireland
6. Training Course in ISO- 15189, 2009,2022 Quality Management, TÜRKAK-SWEDAC
7. Evidence-based Laboratory Medicine, 2008,  IFCC
8. Management and organization 2008, ELGİNKAN
9. Visitor scientist: Ghent Hospital Laboratory (FABRY-POMPE)-5-6 May 2008 –GHENT-Belgium
10. Laboratory statistics, 2002, IFCC
11. ISO 9000-2000 Quality management system 2002, TSE
12. Assesment under stress conditions, 2002, Quality Management, TÜRKAK
13. Framework programme to train assessors in Accreditation procedure, 2002,  TÜRKAK
14. Traceability, validation and verification, 2002,  TÜRKAK
15. Role of lead assessor, behaviour in critical situations, 2002, TÜRKAK
16. ıso 9001-Quality system documentation, 1999,  TSE
17. Peptidergic G-protein coupled receptors: from basic research to clinical applications, 1998, Health and Medicine, NATO
18. Quality management in laboratory 1997, TSE
19. Vascular endothelium: Pharmacologic and genetic manipulations 1996,  NATO
20. Modulation of cellular responses in toxicity, 1994,  NATO
21. Biochemistry of membrane transport 1993, FEBS
22. Techniques in cell biology 1992,  FEBS
23. Basic statistics in medicine, 1992, Ege University
24. Temel Hematoloji ve Biokimya lisansüstü yaz okulu, 1989, , TUBİTAK

DÜZENLEDİĞİ KURS VE SEMPOZYUMLAR

Uluslararası organizasyonlar tarafından desteklenen kurslar

2001; ”Biochemistry education in 2000’s; problems and solutions” sempozyumu düzenleme kurulu üyesi-2001-İzmir

2009: “Evidence based Laboratory Medicine” Kursu-IFCC ve TÜBİTAK destek

2011 “Accreditation and iso15189”- IFCC ve TÜBİTAK destekli

2014  FEBS Advanced Lecture Course-“360 degree Lysosome” FEBS, EMBO, IUBMB ve TUBİTAK destekli

2016: Bölgesel kalkınmada yaşamboyu öğrenmenin rolü çalıştayı-: UALL ve TUBİTAK (ikili işbirliği programı) destekli

2018: e-öğrenme çalıştayı: UALL ve TUBİTAK (ikili işbirliği programı) destekli

2022 FEBS Advanced Lecture Course-“360 degree Lysosome-update” FEBS, EMBO, IUBMB ve TUBİTAK destekli

TÜBİTAK tarafından desteklenen kurslar

1994;  "Endotel" Kursu-TÜBİTAK destekli

1995: "Apoptozis" Kursu-TÜBİTAK destekli

1996: “Calcium:recent developments in clnical and biochemical approaches” Kursu-TÜBİTAK destekli

1998;   ”Birinci basamakta laboratuvar” kursu TTB ve TÜBİTAK destekli

2003-2020 yılları arasında Bilimsel proje yazma kursu – 12 kurs 

2011: “Kanıta dayalı laboratuvar kursu” Kursu-TÜBİTAK destekli

2011; “  iso15189”- kursu TÜBİTAK destekli