İDARİ GÖREVLER

1-İnönü Üniversitesi Tıp Fakültesi Dönem III Koordinatör Yardımcısı. 2000, Malatya.

2- İnönü Üniversitesi Tıp Fakültesi Dönem I Koordinatörü. 2001, Malatya.

3- İnönü Üniversitesi Tıp Fakültesi Dönem III Koordinatörü. 2008, Malatya.

4-İnönü Üniversitesi Tıp Fakültesi Turgut Özal Tıp Merkezi Enfeksiyon Kontrol Kurulu Üyeliği 2006-devam ediyor.

5- İnönü Üniversitesi Tıp Fakültesi Turgut Özal Tıp Merkezi Merkezi Sterilizasyon Sorumlusu, 2012-2016.

6- İnönü Üniversitesi Tıp Fakültesi Turgut Özal Tıp Merkezi Merkez Laboratuvarı Görevi 2000-2018

7- İnönü Üniversitesi Tıp Fakültesi Mezuniyet Öncesi Müfredat Komisyon Üyeliği 2016-2017.

Lisansüstü Tez Yönetimi:

1-Bir yıllık sürede kan kültürlerinin klinik, epidemiyolojik ve bakteriyolojik yönden prospektif olarak değerlendirilmesi. 2005, İnönü Üniversitesi

2- Çürük dişlerde diş plaklarındaki mikroorganizmaların analizi. 2007, İnönü Üniversitesi.

3- Genişlemiş spektrumlu beta laktamaz üreten nozokomiyal Esherichia coli izolatlarnda beta-laktamaz genleri ve klonal ilişkinin araştırılması. 2012, İnönü Üniversitesi.

4- Klinik örneklerden izole edilen MRSA suşlarında vankomisine karşı azalmış duyarlılığın araştırılması. 2015, İnönü Üniversitesi.

Son beş yılda Tıp Fakültesi 1-2-3. sınıflara verilmiş olan dersler

Örneklerin ekime hazırlanması 

Antibiyotik duyarlılık deneyleri 

 Anaerop bakteri infeksiyonları

İmmün yanıtta görev alan organ ve hücreler 

Sitokinler 

Aşılar ve serumlar 

Gram pozitif sporsuz basiller

Sporsuz anaerop bakteriler

Pseudomonas, Burkholderia, Acinetobacter

Gram pozitif sporlu basiller

Sporsuz anaerop bakteriler

Salmonella Shigella

Hastane infeksiyonlarının laboratuar tanısı

Mikolojik tanı için örnek toplama, taşıma ve laboratuar tanısı

Vibrio, Campylobacter, Helicobacter

Tıp Fakültesi dışında ders verilen diğer bölümler

1-Diş Hekimliği Fakültesi

2-Sağlık Hizmetleri Meslek Yüksek Okulu

3-Sağlık Bilimleri Enstitü Yüksek Lisans ve Doktora Eğitim Proğramları (Tıbbi Mikrobiyoloji)

KONFERANS, SEMİNER, PANEL, AÇIK OTURUM, KURS PROGRAMLARINA EĞİTİCİ OLARAK KATILIM

1-Yoğun Bakım Enfeksiyonları Toplantısı 2010, Malatya.

2-KLİMUD(Klinik Mikrobiyoloji Uzmanlık Derneği) Sürekli Tıp Eğitimi Toplantısı, Klinik Örnekten Sonuç Raporuna ‘Steril Vücut Sıvıları’ 2010, Van.

3-Kan Merkezi ve Tranzfüzyon Merkezlerinde çalışan pratisyen hekimlere yönelik pratisyen hekim eğitimi. ‘Transfüzyonla bulaşan enfeksiyon hastalıkları, Mikrobiyolojik tarama ve doğrulama yöntemleri’ 2010 Malatya.

4-Deney Hayvanları Kullanımı ile İlgili Eğitim Sertifikası Kurs Proğramı .’ Temel Laboratuvar Güvenliği ve Temizlik (Atıkların Kontrolü)’. 2011-2015,Malatya.

5-Hastane İnfeksiyonları Eğitim Proğramı, Hastanede salgın yönetimi kursu 2013 Malatya.

6-KLİMUD Sürekli Tıp Eğitimi Toplantısı, Solunum Yolu ve Kan Örnekleri 2013 Malatya.

7-KLİMUD Sürekli Tıp Eğitimi Toplantısı, Yoğun Bakım Ünitesi Enfeksiyonlarına Mikrobiyolojik Yaklaşım-Antimikrobiyal Yönetim 2015, Malatya.

8-KLİMUD Sürekli Tıp Eğitimi Toplantısı, Klinik Örnekten Sonuç Raporuna Uygulama Rehberi: Üriner Sistem Örnekleri 2016, Elazığ.

​

Investigation of Viral and Atypical Pathogens in Patients with Pneumonia Who Need Intensive Care Unit

By

Dogan, A (Dogan, Ahmet) [1] ; Çinar, YE (Cinar, Yasemin Ersoy) [2] ; Otlu, B (Otlu, Baris) [3] ; Kuzucu, Ç (Kuzucu, Cigdem) [4]

 (provided by Clarivate) 

Source

FLORA INFEKSIYON HASTALIKLARI VE KLINIK MIKROBIYOLOJI DERGISI

Volume

27

Issue

1

Page

28-36

DOI

10.5578/flora.20229903

Published

2022

Indexed

2022-05-18

Document Type

Article

Abstract

Introduction: Viral pathogens have been reported increasingly in pneumonia patients. There are few studies in Turkey on viral and atypical bacterial etiology in adult patients with community-acquired pneumonia (CAP) or hospital-acquired pneumonia (HAP). In this study, it was aimed to determine atypical and viral pathogens in patients with pneumonia requiring ICU and to research clinical progression.

Materials and Methods: Adult patients admitted to adult ICUs between November 2016-October 2017 with either CAP or HAP diagnosis were included prospectively. Viral pathogens and also atypical bacterial pathogens were investigated with the in-house multiplex polymerase chain reaction method.

Results: Two hundred patients were enrolled to the study, of whom 63 had CAP (31.5%) and 137 had HAP (68.5%). Viral agents were identified in 31 (15.5%) patients in total, 11 (17.5%) in CAP and 20 (14.6%) in HAP. The most identified viral etiologic agents were rhinovirus, influenza A, and coronavirus HKU. Eight patients (4%) had Mycoplasma pneumoniae. All patients were negative for Legionella pneumoniae and Chlamydophila pneumoniae. Mortality rates were 16.7% for cases with a viral etiology only, 29.2% for cases with bacterial pathogens only, and 23.5% for cases with mixed agents identified.

Conclusion: Viral pathogens and M. pneumoniae should be remembered in the etiology of severe pneumonia patients.

Keywords

Author Keywords

Atypical pneumonia Viral pneumonia Community-acquired pneumonia pneumonia Intensive
 

Assessment of the effectiveness of a ventilator associated pneumonia prevention bundle that contains endotracheal tube with subglottic drainage and cuff pressure monitorization

By

Akdogan, O (Akdogan, Ozlem) [1] ; Ersoy, Y (Ersoy, Yasemin) [1] ; Kuzucu, C (Kuzucu, Cigdem) [2] ; Gedik, E (Gedik, Ender) [3] ; Togal, T (Togal, Turkan) [3] ; Yetkin, F (Yetkin, Funda) [1]

 (provided by Clarivate) 

Source

BRAZILIAN JOURNAL OF INFECTIOUS DISEASES

Volume

21

Issue

3

Page

276-281

DOI

10.1016/j.bjid.2017.01.002

Published

MAY-JUN 2017

Indexed

2017-07-14

Document Type

Article

Abstract

The effectiveness of prevention bundles on the occurrence and mortality of ventilator associated pneumonia (VAP) was evaluated in many studies. However, the effectiveness of endotracheal tube with subglottic secretion drainage (ETT-SD) and cuff pressure monitorization in VAP bundles have not been adequately assessed. In this study, we aimed to evaluate the effectiveness of VAP bundle containing ETT-SD and cuff pressure monitorization. This was a prospective, controlled study that was carried out between March 2011 and April 2012 including intubated patients. The study was conducted at the Anesthesiology Intensive Care Unit 1 and 2 (10 beds each) in a 898-bed university hospital. Occurrence of VAP and compliance with the parameters of the VAP prevention bundles were assessed daily. Patients intubated with the standard endotracheal tube were recruited as controls, mainly in the first six months of the study as ETT-SD and cuff pressure monometer had not yet been implemented. In the second term, patients intubated with ETT-SD were included as cases. Occurrence of VAP, mortality, and compliance with VAP prevention bundles were monitored. A total of 133 patients, 37 cases and 96 controls were recruited. VAP incidence declined from 40.82 to 22.16 per 1000 ventilator days among controls and cases, respectively (p < 005). On average, VAP occurred 17.33 +/- 21.09 days in the case group and 10.43 +/- 7.83 days in the control group (p = 0.04). However, mortality of cases and controls at the 14th and 30th days was not different. VAP prevention bundles including the utilization of ETT-SD, monitoring cuff pressure, and oral care with chlorhexidine were efficient in reducing the rate of VAP. (C) 2017 Sociedade Brasileira de Infectologia. Published by Elsevier Editora Ltda.

Keywords

Ventilator associated pneumonia  VAP prevention bundles Subglottic secretion drainage Cuff pressure monitorizationa

Molecular Characterization of Carbapenem-Resistant Acinetobacter baumannii Blood Culture Isolates from Three Hospitals in Turkey

By

Gozalan, A (Gozalan, Aysegul) [1] ; Unaldi, O (Unaldi, Ozlem) [2] ; Guldemir, D (Guldemir, Dilek) [2] ; Aydogan, S (Aydogan, Sibel) [3] ; Kuzucu, C (Kuzucu, Cigdem) [4] ; Cakirlar, FK (Cakirlar, Fatma Koksal) [5] ; Açikgoz, ZC (Acikgoz, Ziya Cibali) [6] ; Durmaz, R (Durmaz, Riza) [6]

 (provided by Clarivate) 

Source

JAPANESE JOURNAL OF INFECTIOUS DISEASES

Volume

74

Issue

3

Page

200-208

DOI

10.7883/yoken.JJID.2020.478

Published

MAY 2021

Indexed

2021-07-08

Document Type

Article

Abstract

We aimed to investigate the clonal relationships, common sequence types, and carbapenemase genes in 177 non-repetitive blood culture isolates of Acinetobacter baumannii collected from patients at three university hospitals in Turkey in 2016. Molecular epidemiological characteristics of the isolates were examined using pulsed-field gel electrophoresis (PFGE), and multilocus sequence typing (MLST) (Pasteur scheme-cpn60, fusA, gltA, pyrG, recA, rplB, and rpoB). Multiplex PCR was used to investigate the carbapenemase genes, including bla(OXA-23-like),bla(OXA-24-like), bla(OXA-48-like), bla(OXA-58-like), bla(IMP), bla(VIM) and bla(NDm). PFGE genotyping yielded 92 pulsotypes with a clustering ratio of 69.7%. As per a >= 85% similarity coefficient, 159 (90.9%) isolates were found to be clonally related. The bla(OXA-)(23-like) and bla(OXA-)(58)-like genes were identified in 100% and 28.2% of the isolates, respectively. The bla(NDM) gene was identified in two isolates. The MLST analysis included 54 isolates with different pulsotypes, and 29 sequence types (STs). Most of the isolates (n = 36) belonged to the clonal complex (CC)2, one isolate belonged to CC1, and one isolate belonged to CC164. Sixteen new STs (ST1235-ST1250) were identified. Identifying both global ST2 and a large number of new STs, revealed high genetic diversity in A. baumannii isolates in the study population.

Keywords

Keywords Plus

ESCHERICHIA-COLIBETA-LACTAMASES ANTIMICROBIAL RESISTANCE MEDITERRANEAN COUNTRIES HIGH PREVALENCE MULTIPLEX PCR IDENTIFICATION GENES

Is airborne transmission of Acinetobacter baumannii possible: A prospective molecular epidemiologic study in a tertiary care hospital

By

Yakupogullari, Y (Yakupogullari, Yusuf) [1] ; Otlu, B (Otlu, Baris) [1] ; Ersoy, Y (Ersoy, Yasemin) [2] ; Kuzucu, C (Kuzucu, Cigdem) [1] ; Bayindir, Y (Bayindir, Yasar) [2] ; Kayabas, U (Kayabas, Uner) [2] ; Togal, T (Togal, Turkan) [3] ; Kizilkaya, C (Kizilkaya, Canan) [4]

 (provided by Clarivate) 

Source

AMERICAN JOURNAL OF INFECTION CONTROL

Volume

44

Issue

12

Page

1595-1599

DOI

10.1016/j.ajic.2016.05.022

Published

DEC 1 2016

Indexed

2017-02-22

Document Type

Article

Abstract

Background: Understanding the dynamics of aerial spread of Acinetobacter may provide useful information for production of effective control measurements. We investigated genetic relationships between air and clinical isolates of Acinetobacter baumannii in an intensive care unit (ICU) setting.

Methods: We conducted a prospective surveillance study in a tertiary care hospital for 8 months. A total of 186 air samples were taken from 2 ICUs. Clonal characteristics of air isolates were compared with the prospective clinical strains and the previously isolated strains of ICU patients over a 23-month period.

Results: Twenty-six (11.4%) air samples yielded A baumannii, of which 24 (92.3%) isolateswere carbapenemresistant. The Acinetobacter concentrationwas the highest in bedside sampling areas of infected patients (0.39 CFU/m(3)). Air isolateswere clustered in 13 genotypes, and 7 genotypes (including 18 air strains) were clonally related to the clinical strains of 9 ICU patients. One clone continued to be cultured over 27 days in ICU air, and air isolates could be clonally related to 7-week retrospective and approximately 15-week prospective clinical strains.

Conclusions: The results of this study suggest that infected patients could spread significant amounts of Acinetobacter to ICU air. These strains could survive in air for some weeks and could likely still infect new patients after some months. Special control measurements may be required against the airborne spread of Acinetobacter in ICUs. (C) 2016 Association for Professionals in Infection Control and Epidemiology, Inc. Published by Elsevier Inc. All rights reserved.

Keywords

Author Keywords

Intensive care unit Health care-associated infection Aerial spread Environmental sampling

The Effects of Colistin on Imipenem MICs in OXA-48 Producing Klebsiella pneumoniae Isolates: An In Vitro Study

By

Duman, Y (Duman, Yucel) [1] ; Tekerekoglu, MS (Tekerekoglu, Mehmet Sait) [1] ; Kuzucu, Ç (Kuzucu, Cigdem) [2] ; Yakupogullari, Y (Yakupogullari, Yusuf) [1]

 (provided by Clarivate) 

Source

MEDITERRANEAN JOURNAL OF INFECTION MICROBES AND ANTIMICROBIALS

Volume

10

DOI

10.4274/mjima.galenos.2020.2021.2

Article Number

2

Published

2021

Indexed

2021-04-16

Document Type

Article

Jump to

Abstract

Introduction: A new approach to carbapenem resistance-K. pneumoniae infections is to use combination drug therapies. However, little data are available about the effectiveness of the in vitro carbapenem plus colistin combination against oxacillinase-48 (OXA-48) producing K. pneumoniae. Therefore, the aim of this study is to assess the potential synergistic activity of imipenem plus colistin in OXA-48-producing K. pneumoniae strains and investigate the changes in the imipenem minimal inhibitory concentrations (MICs) to varying MICs of colistin.

Materials and Methods: Carbapenem and colistin resistance (CoIR) genes were investigated by polymerase chain reaction. In the first stage, synergistic properties were determined by the checkerboard combination method. In the second step, at varying colistin concentrations, changes in the imipenem MICs were investigated.

Results: Colistin MIC50 2 mu g/ml, MIC90 16 mu g/ml, and imipenem MIC50 32 mu g/ml, MIC90 128 mu g/ml were found, respectively. According to the fractional inhibitor concentration (FIC) formula, 62.2% of the isolates were synergistic, and 37.8% were indifference. When the colistin was fixed at 0.125 mu g/ml, 0.25 mu g/ml, 0.5 mu g/ml, 1 mu g/ml, and 2 mu g/ml, respectively. Significant decreases were observed in the imipenem Mies, especially of colistin-sensitive isolates. However, imipenem MICs of CoIR isolates did not decrease to susceptible levels.

Conclusion: This information will facilitate the design of antibiotic regimens that are more suitable for treating infections due to such pathogens producing OXA-48 and prolong these antibiotics' efficacy. Further in vitro research is required to determine which treatment combination is best and its optimal use as combination therapy to treat these infections.

Keywords

Author Keywords

Colistin imipenem combination Klebsiella OXA-48

Clinical, microbiologic, and epidemiologic characteristics of Pseudomonas aeruginosa infections in a University Hospital, Malatya, Turkey

By

Yetkin, G (Yetkin, Gulay) ; Otlu, B (Otlu, Baris) ; Cicek, A (Cicek, Aysegul) ; Kuzucu, C (Kuzucu, Cigdem) ; Durmaz, R (Durmaz, Riza)

 (provided by Clarivate) 

Source

AMERICAN JOURNAL OF INFECTION CONTROL

Volume

34

Issue

4

Page

188-192

DOI

10.1016/j.ajic.2005.11.010

Published

MAY 2006

Indexed

2006-05-01

Document Type

Article

Abstract

Background: Pseudomonas aeruginosa strains are generally resistant to many antibiotics, and nosocomial infections because of this species are one of the major problems in many hospitals. Molecular typing provides very useful information about origin and transmission of the strains. The aims of the present study were to investigate clinical and microbiologic characteristics of the nosocomial infections caused by P aeruginosa strains in a medical center and to bring up the cross-transmission level of this opportunistic pathogen in a university hospital by analyzing the clonal relationship among the isolates.

Methods: A total of 105 P aeruginosa strains had been identified among the 80 inpatients in a 1-year period from August 2003 to August 2004. Demographic, clinical, and epidemiologic data of the patients were prospectively recorded. The standardized disk-diffusion method was used to determine resistance of the strains to imipenem, ceftazidime, aztreonam, amikacin, gentamicin, mezlocillin, cefepime, tobramycin, meropenem, ceftriaxone, and ciprofloxacin. Clonal relatedness of the strains was investigated by pulsed-field gel electrophoresis (PFGE).

Results: Of the 105 P aeruginosa strains identified, 45 (43%) were isolated from the patients hospitalized in intensive care units. Thirteen patients had repeated pseudomonas infection (total 38 infections/13 patients); 26 of these repeated infections in 9 patients showed the same localization. Half of the patients had at least 1 underlying disease such as burn (48%), chronic illness (32%), and malignancy (20%). Fifty-seven patients (71%) had urinary and/or other catheterization. Urinary tract infection (35 %) was the most frequent infection encountered, followed by respiratory tract infection (34%) and sepsis (13%). Resistance to the antibiotics tested was in the 12% to 88% range; amikacin was the most effective and ceftriaxone was the least effective antibiotic, The PFGE typing method showed that 28 of the 80 patients' isolates were clonally related, including 23 indistinguishable or closely related strains (29%), and 5 possibly related strains (6%). Epidemiologic data of the 16 patients (20% of the patients) confirmed a clonal relationship among the strains. Of the 26 isolates of the 9 patients having repeated infection in the same location, 18 (69%) were in the clonally related groups, whereas 11 of the 12 strains isolated from repeated infections on different body sites were clonally different.

Conclusion: Our results indicated that P aeruginosa infections in our hospital mainly affected the patients hospitalized in intensive care units and those having catheterization, burn, and/or chronic illness. Amikacin was the best antibiotic as far as bacterial resistance was considered. Although lack of major PFGE type confirmed no P aeruginosa outbreak, typing results showed that cross transmission and treatment failure are the 2 main problems, which should be consider together to prevent this bacterial infection in medical centers.

Keywords Plus

OUTBREAK STRAIN

THE EFFECT OF SODIUM DICHLOROISOCYANURATE DIHYDRATE TO PREVENT THE ENVIRONMENTAL TRANSMISSION OF MULTIDRUG-RESISTANT ACINETOBACTER BAUMANNII IN HOSPITAL SETTINGS

By

Duman, Y (Duman, Yucel) [1] ; Kuzucu, C (Kuzucu, Cigdem) [2] ; Ersoy, Y (Ersoy, Yasemin) [3] ; Otlu, B (Otlu, Baris) [1]

 (provided by Clarivate) 

Source

FRESENIUS ENVIRONMENTAL BULLETIN

Volume

29

Issue

9

Page

7191-7197

Published

2020

Indexed

2021-04-25

Document Type

Article

Abstract

Nosocomial infections are a substantial concern as the major cause of morbidity and mortality of hospitalized patients' in the world. Disinfection of inanimate environment, equipment and hospital setting is important to prevent nosocomial infections. Sodium dichloroisocyanurate dihydrate (NaDCC) can he used for disinfection of environment and medical devices. The aims of this study were to determine the efficacy of NaDCC at various concentrations and times against multi-drug resistant Acinetobacter strains.

In the first phase of the study, the bactericidal activity of NaDCC to A. baumannii was investigated by quantitative suspension test, In the second phase, the surface activity of NaDCC was tested by surface disinfection application test. In the third phase, before the cleaning of randomly selected patient's room A. baumannii contamination on the inanimate environment objects and equipment was investigated. After the cleaning of the room the effect of NaDCC was tested.

As a result of the quantitative suspension test; NaDCC was inhibited the all A. baumannii and ATCC strains. In the surface disinfection application test, it was determined that at the concentration of 1000 ppm and 500 ppm, the activity of NaDCC; at 5th, 30th and 60th minutes was effective to micro-organisms at 5 log level, respectively. But at 100 ppm concentration it was effective to at 5th minutes three. at 30th and 60th minutes seven A. baumannii strains at 5 log levels, while it was effective at log 1 level to other A. baumannii strains and S. aureus, E. colt and P. aeruginosa ATCC. As a result of investigation the A. baumannii contamination in patient's room; before the cleaning, we determined A. baumannii contamination on the inanimate objects of room (such as bed surface, bed edges, control device, nightstand, chair) and on equipment (such as stethoscope, steam appliance, blood pressure device, aspirator heads, ventilator surfaces). After the cleaning it was determined that at 1000 ppm concentration at 5th, 30th and 60th minutes NaDCC was effective to A. baumannii at 5 log levels, However, at 500 ppm concentration at 5th minute it was effective at log 5 level except control device. At 30th and 60th minutes of 500 ppm concentration of NaDCC was effective at log 5 level to A. baumannii, At 100 ppm concentration at 5th, 30th and 60th minutes it was effective to A. baumannii strains at log 1 level on inanimate objects and equipment.

In low concentration, NaDCC efficacy was reduced against A. baumannii. The application concentration and time of the disinfectant to clean up the equipment and the environment is very important for preventing nosocomial infections and the spread of A. baumannii. Thus, it is necessary to check and follow up the staff and to create clean and disinfection training programs for educating staff.

Keywords

Author Keywords

Nosocomial infections Disinfectants Acinetobacter baumannii

Species distribution, antifungal susceptibility and clonal relatedness of Candida isolates from patients in neonatal and pediatric intensive care units at a medical center in Turkey

By

Kuzucu, C (Kuzucu, Cigdem) [1] ; Durmaz, R (Durmaz, Riza) [1] ; Otlu, B (Otlu, Baris) [1] ; Aktas, E (Aktas, Elif) [2] ; Gulcan, H (Gulcan, Hande) [3] ; Cizmeci, Z (Cizmeci, Zaynep) [4]

 (provided by Clarivate) 

Source

NEW MICROBIOLOGICA

Volume

31

Issue

3

Page

401-408

Published

JUL 2008

Indexed

2008-07-01

Document Type

Article

Abstract

The aim of this study was to assess species distribution, antifungal susceptibility and clonal relationships among Candida strains isolated from a group of pediatric/neonatal intensive care (PICU/NICU) patients that had a very high mortality rate (76%). The cases of 21 patients (19 with candidemia, 2 with Candida meningitides) treated over a 1-year period in a Turkish hospital PICU and NICU were retrospectively analyzed. Twenty-eight Candida isolates were detected from blood (20), cerebrospinal fluid (CSF) (2) and other specimens (6). Candida species were identified using the API ID 32C System. Susceptibility testing was done (all 28 isolates) for amphotericin B, fluconazole and itraconazole using the broth microdilution method. Arbitrarily primed polymerase chain reaction (AP-PCR) was used for molecular typing of the 3 most common ones; C. albicans (15), C. parapsilosis (6), and C. pelliculosa (4). Electrophoretic karyotyping (EK) was done to check clonal identity obtained by AP-PCR. Of the 20 blood isolates, 8 (40%) were C. albicans, 12 (60%) were non-albicans Candida, and one of the 2 CSF isolates was C. albicans. The overall species distribution was as follows: 15 C. albicans isolates, 6 C. parapsilosis isolates, 4 C. pelliculosa isolates, 2 C. famata isolates and I C. tropicalis isolate. Amphotericin B had the best antifungal activity with a MIC90 of 0.125 mu g/ml, and the rates of susceptibility to fluconazole and itraconazole were 93% and 82%, respectively AP-PCR revealed 11 genotypes (4 were identical pairs, 7 were distinct) among the 15 C albicans isolates, 2 genotypes (5 were classified in the same type) among the 6 C. parapsilosis isolates, and 4 separate genotypes for the 4 C. pelliculosa isolates. Karyotyping results correlated well with the AP-PCR findings. As indicated in the previous research, our results confirmed that non-albicans Candida species have become more frequently causative agents for invasive fungal infections in the ICU. Transmission of C. albicans and C pelliculosa was relatively low, but transmission of C. parapsilosis was high, suggesting that more effective control and very strict treatment protocols are needed for patients having high mortality and invasive fungal infection in ICU.

Keywords

Author Keywords

bloodstream infections Candida spp. neonatal intensive care unit arbitrarily primed polymerase chain reaction electrophoretic karyotyping antifungal susceptibility testing

MRSA genotypes in Turkey: Persistence over 10 years of a single clone of ST239

By

Alp, E (Alp, Emine) [1] ; Klaassen, CHW (Klaassen, Corne H. W.) [2] ; Doganay, M (Doganay, Mehmet) [1] ; Altoparlak, U (Altoparlak, Ulku) [3] ; Aydin, K (Aydin, Kemalettin) [4] ; Engin, A (Engin, Aynur) [5] ; Kuzucu, C (Kuzucu, Cigdem) [6] ; Ozakin, C (Ozakin, Cuneyt) [7] ; Ozinel, MA (Ozinel, Mehmet Ali) [8] ; Turhan, O (Turhan, Ozge) [9] ; 

 (provided by Clarivate) 

Source

JOURNAL OF INFECTION

Volume

58

Issue

6

Page

433-438

DOI

10.1016/j.jinf.2009.04.006

Published

JUN 2009

Indexed

2009-06-01

Document Type

Article

Abstract

Objectives: Methicillin-resistant Staphylococcus aureus (MRSA) is a significant cause of life-threatening human infections. The clinical impact of MRSA is mounting, not only due to the ever-increasing prevalence but also due to the occurrence of new, community-acquired MRSA strains. The aim of this prospective, multi-centre study was to determine the prevalence and genetic relatedness of clinically relevant MRSA isolates, in Turkey.

Methods: During a 1-year period, data from 20 successive patients with invasive S. aureus infections were collected from eight university hospitals, geographically distributed over the six main regions of Turkey. Among these S. aureus isolates, the genetic association of MRSA isolates was investigated by pulsed-field get electrophoresis (PFGE) and spa typing. A selected number of isolates were also analyzed by multilocus sequence typing (MLST). Furthermore, Panton Valentine leucocidin (PVL) genes were examined.

Results: In this study, the rate of methicillin resistance in S. aureus in patients with apparent infections (sepsis, meningitis, lung abscess or septic arthritis) ranged from 12 to 75% within the seven participating centres. Typing by pulsed-field get electrophoresis and spa typing revealed the presence of 22 closely related genotypes. According to the PFGE and spa typing results, 53 out of 54 MRSA isolates were closely related. These isolates were of spa type t030 or a related spa type, contain an SCC mec type III element and belong to sequence type ST239. None of the isolates contained the PVL genes.

Conclusions: Despite the broad surface area of Turkey, a single predominant clone of ST239 circulates in hospitals in different regions and only few new types of MRSA were introduced over the past years. These results place Turkey in the epicenter of ST239 prevalence. (C) 2009 The British Infection Society. Published by Elsevier Ltd. All rights reserved.

Keywords

Author Keywords

Methicillin-resistant Staphylococcus aureus Molecular epidemiology Panton Valentine leucocidin genes

THE IDENTIFICATION OF ACINETOBACTER BAUMANNII BLOOD ISOLATES BY MALDI-TOF MS, ARDRA AND BLA_OXA-51-LIKE GENE-SPECIFIC REAL-TIME PCR

By

Gözalan, A (Gozalan, Aysegul) [1] ; Aydogan, S (Aydogan, Sibel) [2] ; Haciseyitoglu, D (Haciseyitoglu, Demet) [3] ; Kuzucu, Ç (Kuzucu, Cigdem) [4] ; Köksal, F (Koksal, Fatma) [5] ; Açikgöz, ZC (Acikgoz, Ziya Cibali) [6] ; Durmaz, R (Durmaz, Riza) [6]

 (provided by Clarivate) 

Source

MIKROBIYOLOJI BULTENI

Volume

54

Issue

4

Page

535-546

DOI

10.5578/mb.70085

Published

SEP 2020

Indexed

2020-11-09

Document Type

Article

Abstract

The Acinetobacter cakoaceticus-Acinetobacter baumannii (Acb) complex consists of phenotypically very similar nosocomial species; A.baumannii, Acinetobacter nosocomialis, Acinetobacter pittii, Acinetobacter seifertii and Acinetobacter djikshoorniae and one environmental species Acakoaceticus. The rapid and accurate identification of the members of Acb complex is critical as these nosocomial pathogens can show differences in antimicrobial susceptibility and clinical outcomes. The conventional phenotypic methods are slow, unreliable and less efficient for the differentiation of Acb complex species, including the A.baumannii species within the Acb complex. Although various molecular methods are available, such as amplified ribosomal DNA restriction analysis (ARDRA) and bla(OXA-51-like), gene specific PCR, they are usually inconvenient for the routine diagnostic laboratories. Recently, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) offers an opportunity for rapid, cost-effective and convenient bacterial identification in routine diagnostic procedures conducted in clinical laboratory. In this study, we aimed to evaluate the diagnosis performance of MALDI-TOF MS system to identify blood isolates of A.baumannii. A total of 180 nonduplicate carbapenem resistant Acb complex (strain numbers; TR1-TR60) and A.baumannii (TR61-TR180) blood isolates were collected from the intensive care units of the three university hospitals in Turkey from January 2016 to December 2016. All isolates were evaluated by using bla(OXA-51-like) gene specific real time (Rt-PCR) analysis, ARDRA (restriction enzymes-AIM, Cfol, Mbol, Mspl, Rsal) method and MALDI-TOF MS (VITEK (R) MS, bioMerieux, France) system. All the strains except TRIO, TR31, TR35 and TR52 were identified as A.baumannii by ARDRA method. Out of 177 of all the isolates, presence of bla(OXA-51-like) gene was found except for TR10, TR31 and TR52 isolates. However, TR31 without the presence of bla(OXA-51-like) gene was identified as A.pittii using the ARDRA. Totally 176 isolates which were identified as A. baumannii by both of the methods, ARDRA and Rt-PCR-bla(OXA-51-like) ,were accepted as a reference for the evaluation of the diagnosis performance capacity of the MALDI-TOF MS. Overall, for all 176 isolates tested, the sensitivity obtained with the MALDI-TOF MS were 99.4% with 75% specificity. The accuracy value of the method was determined as 98.9% for the identification of A.baumannii to the species level. MALDI-TOF MS is increasingly used in diagnostic microbiology for the routine identification of bacteria to the genus, species or subspecies level with high rates of sensitivity and specificity. In future, by expanding the database, MALDI-TOF MS system would possibly become the ideal method for routine diagnostic laboratories that could potentially identify more species and even determine some characteristics of antimicrobial resistance and virulence determinants.

Keywords

Author Keywords

Acinetobacter baumannii polymerase chain reaction amplified ribosomal DNA restriction analysis matrix-assisted laser desorption-ionization mass spectrometry

Investigation of Beta-Lactamase Genes and Clonal Relationship Among the Extended-Spectrum Beta-Lactamase Producing Nosocomial Escherichia coli Isolates

By

Görgeç, S (Gorgec, Sunduz) [1] ; Kuzucu, Ç (Kuzucu, Cigdem) [2] ; Otlu, B (Otlu, Baris) [2] ; Yetkin, F (Yetkin, Funda) [3] ; Ersoy, Y (Ersoy, Yasemin) [3]

 (provided by Clarivate) 

Source

MIKROBIYOLOJI BULTENI

Volume

49

Issue

1

Page

15-25

Published

JAN 2015

Indexed

2015-04-15

Document Type

Article

Abstract

Extended-spectrum beta-lactamase (ESBL) producing microorganisms currently cause a major problem. Among theseCTX-M beta-lactamase producing Escherichia coli has also disseminated worldwide as an important cause of both nosocomial and community-acquired infections. The aims of this study were to determine the prevalence of the beta-lactamase genes, antibiotic susceptibilities and clonal relationships of ESBL-producing nosocomial E.coli isolates. A total of 76 ESBL-producing E.coli strains isolated from urine (n= 26), blood (n= 25) and wound (n= 25) specimens of hospitalized patients identified as nosocomial infection agents according to the CDC criteria between June 2010-June 2011 were included in the study. Antibiotic susceptibilities of the isolates were detected by Kirby-Bauer disc diffusion method according to CLSI recommendations. ESBL production was tested by double disc diffusion method, and cefotaxime/cefotaxime-clavulanic acid E-test strips (AB Biodisk, Sweden) were used for indeterminate results. Presence of TEM, SHV, CTX-M, OXA-2 group, OXA-10 group, PER, VEB and GES beta-lactamase genes were investigated by polymerase chain reaction (PCR) using specific primers. Pulsed-field gel electrophoresis (PFGE) method was used for the detection of clonal relationships among the strains. Most of the ESBL-producing E.coli strains were isolated from samples of inpatients in intensive care (35%), internal medicine (16%) and general surgery (13%) units. All of the 76 strains were found susceptible to imipenem, meropenem and amikacin; however all were resistant to cefotaxime and ceftriaxone. The susceptibility rates of the isolates to cefoxitin, ertapenem, cefoperazone/sulbactam, piperacillin-tazobactam, gentamicin, ciprofloxacin, cefepime, amoxicillin-clavulanic acid, aztreonam and ceftazidime were 96%, 83%, 63%, 61%, 50%, 41%, 25%, 21%, 20% and 18%, respectively. Among E.coli isolates, the frequency of CTX-M, TEM, OXA-2 group, PER, SHV and OXA-10 group beta-lactamase genes were found as 89.5%, 59.2%, 15.8%, 14.5%, 11.8% and 3.9%, respectively, while none of the isolates were positive for VEB and GES beta-lactamase genes. In 1 (1.3%) strain none of the investigated genes were detected. PCR analyses of the isolates revealed that 25 harbored CTX-M and TEM genes together, while 20 harbored only CTX-M and two harbored only TEM genes. Single SHV gene was not detected in any of the isolates. PFGE demonstrated no major clonal relationship between ESBL-producing isolates. This study indicated that CTX-M type enzymes were highly endemic among ESBL-producing nosocomial E.coli strains in our hospital, with the polyclonal spread of ESBL-producing bacteria without any dominant epidemic clone. In conclusion, it was considered that further studies are needed to explain the relationship between epidemic clones and plasmids with the use of plasmid analysis and multilocus sequence typing methods.

Keywords

Author Keywords

Escherichia colibeta-lactamase CTX-M clonal relationship

AN OUTBREAK ASSOCIATED WITH MULTIDRUG-RESISTANT PSEUDOMONAS AERUGINOSA CONTAMINATION OF DUODENOSCOPES AND AN AUTOMATED ENDOSCOPE REPROCESSOR

By

Yetkin, F (Yetkin, Funda) [1] ; Ersoy, Y (Ersoy, Yasemin) [1] ; Kuzucu, C (Kuzucu, Cigdem) [2] ; Otlu, B (Otlu, Baris) [2] ; Parmaksiz, N (Parmaksiz, Nalan) [3] ; Seckin, Y (Seckin, Yuksel) [4]

 (provided by Clarivate) 

Source

BIOMEDICAL RESEARCH-INDIA

Volume

28

Issue

13

Page

6064-6070

Published

2017

Indexed

2017-08-24

Document Type

Article

Abstract

Background: Duodenoscopes are semi-critical devices used for endoscopic retrograde cholangiopancreatography (ERCP). Disinfection of these instruments is usually based on high-level disinfection procedures with a manual or automated endoscope reprocessor (AER). Duodenoscopes and AER are reported very rarely as a source of infection and outbreaks.

Aim: To investigate an outbreak caused by Pseudomonas aeruginosa in a Gastroenterology Department and ERCP unit in a university hospital and its underlying risk factors.

Method: Three patients in the gastroenterology unit were diagnosed as infected by multidrug-resistant P. aeruginosa and a case control study was conducted for detection of the risk factors. Our infection control team commenced active epidemiological surveillance to determine the cause of these infections. Clonal relationship of the strains was investigated by pulsed field gel electrophoresis (PFGE).

Results: Eight patients were affected in the gastroenterology unit during the period November 2007-February 2008. The case-control analysis confirmed that undergoing ERCP was significantly associated with isolation of P. aeruginosa (P=0.0001) in this unit. Six patients' isolates and seven environmental isolates had an indistinguishable PFGE profile, confirming cross-transmission. The healthcare worker implemented infection control measures to resolve the outbreak and no further cases occurred.

Conclusions: This outbreak resulted from failure of AER and inadequate high level disinfection procedures. AERs can cause contamination of duodenoscopes and can be related P. aeruginosa outbreaks. Reuse of ancillary materials of ERCP play a critical role in outbreak development.

Author Keywords

Multidrug-resistant P. aeruginosa Nosocomial infection Outbreak Pulsed field gel electrophoresis (PFGE)Endoscopic retrograde cholangiopancreatography (ERCP)

Pathogens of Intensive Care Unit-Acquired Infections and Their Antimicrobial Resistance: A 9-Year Analysis of Data from a University Hospital

By

Yetkin, F (Yetkin, Funda) [1] ; Yakupogullari, Y (Yakupogullari, Yusuf) [2] , [3] , [4] ; Kuzucu, C (Kuzucu, Cigdem) [2] , [3] , [4] ; Ersoy, Y (Ersoy, Yasemin) [1] ; Otlu, B (Otlu, Baris) [2] , [3] , [4] ; Colak, C (Colak, Cemil) ; Parmaksiz, N (Parmaksiz, Nalan)

 (provided by Clarivate) 

Source

JUNDISHAPUR JOURNAL OF MICROBIOLOGY

Volume

11

Issue

10

DOI

10.5812/jjm.67716

Article Number

e67716

Published

OCT 2018

Indexed

2018-11-02

Document Type

Article

Abstract

Background: Pathogens surveillance and antimicrobial resistance are essential for the prompt organization of therapeutic and preventive actions in healthcare settings.

Objectives: We investigated the causative agents of intensive care unit (ICU)-acquired infections and their antimicrobial resistance in a university hospital over a nine-year period.

Methods: An active, prospective surveillance was conducted in the ICUs of a tertiary care hospital between 2007 and 2015. The changing patterns in the frequency of pathogens and their antimicrobial resistance by the time were statistically evaluated with Mann-Whitney U test.

Results: A total of 3044 pathogens were isolated from 4272 healthcare-associated infections attacks in 3437 patients. The most frequently detected organisms were Acinetobacter spp. (n = 1060, 34.8%), Pseudomonas aeruginosa (n = 622, 20.4%), Escherichia coli (n = 340, 11.1%), Klebsiella pneumoniae (n = 331, 10.8%), and Candida spp. (n = 285, 9.3%). Carbapenem resistance among Acinetobacter spp., P. aeruginosa, E. coli, and K. pneumoniae was found as 82%, 30.7%, 2%, and 9.3%, respectively. The prevalence of extended-spectrum betalactamase (ESBL) among E. coli and K. pneumoniae was 49.7% and 41.3%, orderly, and methicillin resistance in Staphylococcus aureus was 81.8%. Substantial reductions occurred in the rates of E. coli (16.8% to 8.9%), S. aureus (11% to 3.2%), coagulase-negative staphylococci (7.9% to 0), and Stenotrophomonas maltophilia (4.2% to 0.3%) during the study period by the applied infection control measures while the rate of Acinetobacter spp. (9.7% to 51%) significantly increased. Furthermore, the increases in the carbapenem resistance among Acinetobacter spp. (52.5% to 91.4%), Pseudomonas spp. (25.7% to 51.6%), E. coli (0 to 12.7%), and K. pneumoniae (2.6% to 9%) and the decrease in the prevalence of ESBL-producing E. coli (57% to 27.2%) were statistically significant.

Conclusions: Despite the decreases in the frequencies of staphylococci and some Gram-negative bacteria, the current infection control measures have been unable to limit the spread of carbapenem-resistant Gram-negative bacteria in our facility. Additional precautions are required to control such pathogens in the intensive care units.

Keywords

Author Keywords

Hospital-Acquired Infection Gram-Negative Bacteria Carbapenem Resistance

Evaluation of Nosocomial Infections after ABO-Compatible and Incompatible Liver Transplantations

By

Bayar, FS (Bayar, Filiz Surucu) [1] ; Bayindir, Y (Bayindir, Yasar) [2] ; Isik, B (Isik, Burak) [3] ; Özgör, D (Ozgor, Dincer) [3] ; Kayabas, U (Kayabas, Uner) [4] ; Kuzucu, Ç (Kuzucu, Cigdem) [5] ; Yilmaz, S (Yilmaz, Sezai) [3]

 (provided by Clarivate) 

Source

MEDITERRANEAN JOURNAL OF INFECTION MICROBES AND ANTIMICROBIALS

Volume

7

DOI

10.4274/mjima.2018.14

Article Number

14

Published

2018

Indexed

2019-04-18

Document Type

Article

Abstract

Introduction: Liver transplantation is currently the only treatment option in acute hepatic failure and end-stage liver disease. In spite of their complications, ABO-incompatible liver transplantations are performed due to the shortage of suitable donors and the urgent need for organs. Despite developments in surgical techniques and improvements in antimicrobial prophylaxis strategies, infection is still an important complication and continues to be a major cause of death. In this study, ABO-compatible and ABO-incompatible liver transplant recipients were compared retrospectively in terms of infections and survival.

Materials and Methods: Sixteen ABO-incompatible liver transplant recipients whose transplant surgeries were performed by the Liver Transplant Team between March 2002 and January 2011 were included in the study. ABO-compatible liver transplant recipients whose surgeries were undergone before and after the ABO-incompatible transplantation were selected as control group (total 32 patients). Patients' postoperative one year data were obtained from hospital records in both groups.

Results: In the first postoperative year after liver transplantation, 12 (75%) of the ABO-incompatible recipients and 21 (65.6%) of the ABO-compatible recipients experienced at least one infection attack (p=0.509). The infection attack rate was 175% in the ABO-incompatible group and 113% in the ABO-compatible group (p=0.262). Eight (50%) of the 16 ABO-incompatible recipients and nine (28.1%) of the 32 ABO-compatible recipients died within one year of transplantation (p=0.135). There were no statistically significant differences between the groups in terms of mortality or infection rates. Pseudomonas aeruginosa was the most common isolate in both ABO-compatible and incompatible recipients.

Conclusion: The findings of the study showed that ABO-incompatible transplants were comparable to ABO-compatible transplants in terms of infection rates and survival. It should be kept in mind that ABO-incompatible liver transplantation may be an option, especially in emergencies and in selected cases, despite differences in complications between centers and patient groups and concerns about greater complications in ABO-incompatible patients. However, further studies are needed on this topic.

Author Keywords

ABO-incompatible and compatible liver transplantation infection survival

BRUCELLA AND PERITONEAL DIALYSIS RELATED PERITONITIS: CASE REPORT AND REVIEW OF LITERATURE

By

Koz, S (Koz, Suleyman) [1] ; Sahin, I (Sahin, Idris) [1] ; Kayabas, U (Kayabas, Uner) [2] ; Kuzucu, C (Kuzucu, Cigdem) [3]

 (provided by Clarivate) 

Source

CLINICAL NEPHROLOGY

Volume

82

Issue

4

Page

283-286

DOI

10.5414/CN107828

Published

OCT 2014

Indexed

2015-02-25

Document Type

Review

Abstract

Isolated case reports of peritonitis due to Brucella spp. during peritoneal dialysis (PD) continue to surface in the medical literature. However, the optimal treatment regimen for these patients, in particular with regards to the fate of PD catheter, is still largely unknown. We report a case of brucella peritonitis successfully treated with intraperitoneal administration of amikacin, along with oral rifampicin and doxycycline but without catheter removal. Furthermore, we have reviewed the literature up until present day.

Keywords

Author Keywords

Brucella peritoneal dialysis peritonitis

Investigation of the Susceptibilities of Extended-Spectrum Beta-Lactamase Producing Escherichia coli and Klebsiella spp. Strains to Ertapenem and Other Carbapenems

By

Kuzucu, Ç (Kuzucu, Cigdem) [1] ; Yetkin, F (Yetkin, Funda) [2] ; Görgeç, S (Gorgec, Sunduz) [1] ; Ersoy, Y (Ersoy, Yasemin) [2]

 (provided by Clarivate) 

Source

MIKROBIYOLOJI BULTENI

Volume

45

Issue

1

Page

28-35

Published

JAN 2011

Indexed

2011-01-01

Document Type

Article

Abstract

Infections caused by extended-spectrum beta-lactamase (ESBL) producing Escherichia coli and Klebsiella spp. constitute severe problems. Carbapenems are commonly used to treat these infections. However, infections caused by carbapenem-resistant gram-negative bacteria show an increasing trend recently. The aim of this study was to investigate the susceptibilities of ESBL-producing E.coli and Klebsiella spp. to ertapenem and other carbapenems. A total of 239 E.coli, 28 K.pneumoniae and 11 K.oxytoca strains isolated from clinical specimens (208 urine, 16 blood, 26 wound, 17 sterile body fluids, four tracheal aspirates and seven others) of hospitalized patients and outpatients between January 2007-February 2008, were included to the study. The isolates were identified by conventional methods, and antibiotic susceptibility tests were performed by Kirby Bauer disc diffusion method according to Clinical and Laboratory Standards Institute (CLSI) standards. ESBL production was tested by double disk diffusion method. When ESBL production was indeterminate, cefotaxime-clavulanic acid E test (BioMerieux, France) was used. According to the CLSI standards modified Hodge test was performed for carbapenem resistant isolates and minimal inhibitory concentration (MIC) values were detected for ertapenem (Etest (R), BioMerieux, France), imipenem and meropenem (M.I.C. Evaluator Strips, Oxoid, UK). All of the isolates were found susceptible to amikacin (278/278; 100%), whereas the susceptibility rates for imipenem/meropenem and ertapenem were 99.3% (276/278) and 98.6% (274/278), respectively. When evaluated individually, ertapenem susceptibilities of E.coli, K.pneumoniae and K.oxytoca strains were 99.2%, 96.4% and 90.9%, respectively, while these rates were 100%, 96.4% and 90.9%, respectively, for imipenem/meropenem. Carbapenem resistance was detected in two E.coli, one K.oxytoca and one K.pneumoniae isolates. While two Klebsiella spp. isolates were resistant to all of the tested carbapenems (MIC > 32 mu g/ml), two E.coli isolates were resistant to ertapenem (MIC > 32 mu g/ml) but susceptible to imipenem (MIC= 0.25 mu g/ml) and meropenem (MIC= 0.5 mu g/ml). Carbapenemase production was demonstrated by modified Hodge test in all of the carbapenem-resistant isolates. In conclusion, ESBL-producing gram-negative isolates should be routinely tested with a screening method for carbapenemase activity and confirmation tests should be performed in suspected cases.

Author Keywords

Ertapenem ESBL carbapenemase modified Hodge test

Fungus Ball in Concha Bullosa: An Unusual Cause of Retro- orbital Pain

By

Toplu, Y (Toplu, Yuksel) [1] ; Toplu, SA (Toplu, Sibel A.) [2] ; Can, S (Can, Sermin) [1] ; Kuzucu, C (Kuzucu, Cigdem) [3]

 (provided by Clarivate) 

Source

JOURNAL OF CRANIOFACIAL SURGERY

Volume

25

Issue

2

Page

E138-E140

DOI

10.1097/SCS.0000000000000472

Published

MAR 2014

Indexed

2014-06-04

Document Type

Article

Abstract

Fungus ball in the concha bullosa is an extremely rare disease. We described a case of the fungus ball in the concha bullosa in a 22-year-old woman. Preoperative diagnosis was based on nasal endoscopy and computed tomography scanning. The patient was endoscopically operated on. The examination of the removed material was reported as fungal infection. This case was found worth writing because of the location of the concha bullosa and its rare occurrence in this location.

Keywords

Author Keywords

Fungus ball concha bullosa endoscopic sinus surgery

Bacteria Isolated from Blood Cultures and Their Antimicrobial Susceptibility

By

Duman, Y (Duman, Yucel) [1] ; Kuzucu, Ç (Kuzucu, Cigdem) [1] ; Çuogan, SS (Cuogan, Serpil Semiha) [1]

 (provided by Clarivate) 

Source

ERCIYES MEDICAL JOURNAL

Volume

33

Issue

3

Page

189-196

Published

SEP 2011

Indexed

2011-09-01

Document Type

Article

Abstract

Purpose: The aim of this study was to analyse the distrubution and antimicrobial susceptibility stream infections in 2009.

Material and Methods: The samples were incubated in BACT / ALERT 3D automated systems for five days. Microorganisms were identified by using conventional methods. Antibiotic susceptibility was determined by Kirby-Bauer disk diffusion method according to the recommentations of Clinical Laboratory Standards Institute (CLSI) criteria.

Results: The microorganisms isolated were 31.5% Gram-negative and 68.5% Gram-positive bacteria. The most frequently isolated bacteria were Escherichia coli and coagulase negative staphylococcus (CN5). All of the E.coli and Klebsiella were susceptible to imipenem, meropenem and amikacin. imipenem was the most effective antibiotic against Pseudomonas aerugiosa, and tigecycline was the most effective antibiotic against Acinetobacter spp. in this study 30.8% of Staphylococcus aureus isolates were resistant to methicillin, no glycopeptide resistant Staphylococci was encountered. One enterecoccus strain was resistant to glycopeptides.

Conclusion: Identification of microorganisms from blood cultures and its antibiotic susceptibility pattern will guide to clinician during the treatment.

Author Keywords

Bacteremia Gram-Pozitive Bacteria Gram-Negative Bacteria Bacterial Sensitivity Test

An Uncommon Case of Acute Brucellosis Presenting with Severe Thrombocytopenia

By

Dal, SE (Dal, Sirvan Elmas) [1] ; Ersoy, Y (Ersoy, Yasemin) [1] ; Erkurt, MA (Erkurt, Mehmet Ali) [2] ; Yetkin, F (Yetkin, Funda) [1] ; Kuzucu, C (Kuzucu, Cigdem) [3] ; Akdogan, O (Akdogan, Ozlem) [1]

 (provided by Clarivate) 

Source

INTERNAL MEDICINE

Volume

51

Issue

23

Page

3291-3293

DOI

10.2169/internalmedicine.51.7365

Published

2012

Indexed

2013-03-06

Document Type

Article

Abstract

A 49-year-old man was admitted to the hospital with complaints of fatigue, epistaxis and a skin rash. The whole blood count revealed isolated thrombocytopenia (4,000/mL), and the patient was admitted to the hematology department with a diagnosis of immune thrombocytopenia. He did not respond to steroid treatment for 15 days, and a subfebrile fever developed during this period. A diagnosis of acute brucellosis was considered due to positive serological tests and a blood culture positive for Brucella spp. After starting doxycycline and rifampicin therapy, the patient's thrombocyte count increased to 15,000/mL on the third day, to 41,000/mL on the sixth day and was normal on the 21st day of treatment. A diagnosis of brucellosis must be considered in patients presenting with severe and isolated thrombocytopenia in countries where brucellosis is endemic.

Keywords

Author Keywords

thrombocytopenia brucellosis immune

Antifungal susceptibilities and subtype identifications of candida species, isolated in blood cultures throughout one-year period

By

Kuzucu, C (Kuzucu, Cigdem) [1] ; Yetkin, G (Yetkin, Gulay) [1] ; Caliskan, A (Caliskan, Ahmet) [1]

 (provided by Clarivate) 

Source

ERCIYES MEDICAL JOURNAL

Volume

29

Issue

2

Page

115-119

Published

JUN 2007

Indexed

2007-06-01

Document Type

Article

Abstract

Purpose: In intensive care units, 10-20% of nosocomial bloodstream infections are composed of Candida species. Candida species of normal flora omits the skin barrier by the way of invasive catheter application or endothracheal intubation and cause infections. In this study, we aimed to determine the distribution and the antifungal susceptibilities of Candida species isolated from blood cultures.

Material Methods: Amphotericin B, fluconasole and itraconazole susceptibilities and Minimum Inhibitory Concentrations (MIC values) were measured with M27-A2 broth microdilution method which is recommended by National Committee for Clinical Laboratory Standards (NCCLS).

Results: Candida spp were isolated in blood cultures of 23 patients throughout one-year period. Six of them were children and 17 were adults. 16 patients (69%) were admitted at intensive care unit. The most prevalent agent was C. albicans (52%). Amphotericin MIC values were found pretty low. Fluconazole susceptibility was detected 96% in accordance with the other studies.

Conclusion: Due to the highest mortality rate of candidemia, especially candida infections emerged in intensive care units should be followed in case of appropriate and sufficient antifungal treatment and the emergence of resistant Candida species.

Author Keywords

Antifungal agents CandidaDrug resistance fungal

Molecular typing of methicillin-resistant Staphylococcus aureus isolated from bloodstream infections in a university hospital

By

Yetkin, G (Yetkin, Guelay) [1] ; Kuzucu, C (Kuzucu, Cigdem) [1] ; Durmaz, B (Durmaz, Benguel) [1] ; Durmaz, R (Durmaz, Riza) [1] , [2] ; Çizmeci, Z (Cizmeci, Zeynep) [1] ; Iseri, L (Iseri, Latife) [1]

 (provided by Clarivate) 

Source

TURKISH JOURNAL OF MEDICAL SCIENCES

Volume

39

Issue

6

Page

959-968

DOI

10.3906/sag-0801-12

Published

DEC 2009

Indexed

2009-12-01

Document Type

Article

Abstract

Aim: Bloodstream infections due to methicillin-resistant Staphyococcus aureus (MRSA) strains are one of the major problems in many hospitals. Molecular typing provides very useful information about the origin and the spreading ways of strains. The aim of the present study is to assess the clonal relationship amongst MRSA strains isolated from bloodstream infections of patients in Turgut Ozal Medical Center, Inonu University in Turkey.

Materials and methods: A total of 37 consecutive MRSA strains were identified from the blood cultures from January to December 2003. Methicillin resistance was confirmed with amplification of the mecA gene by polymerase chain reaction (PCR). Clonal relatedness of the strains was investigated by arbitrarily primed polymerase chain reaction (AP-PCR) and pulsed-field gel electrophoresis (PFGE).

Results: Of the 37 mecA positive S. aureus strains identified in a 1-year period, 29 (78.30% ) were from intensive care units (ICUs) and the remaining 6 from other wards. The MRSA strains were resistant to most clinically useful anti-staphylococcal agents. AP-PCR and PFGE typing methods indicated that 67.6% and 60.7% of the typed strains were clonally related, respectively. Clonally related strains were not restricted in a specific clinic and period.

Conclusion: Our findings indicated that MRSA bloodstream infections in our hospital were not originated from any predominant clone and AP-PCR typing can be used to screen clonal relatedness of these strains. The present data showed that there was no predominant MRSA clone in our hospital. However, because of the high rates of MRSA and clonally related strains, the infection control practices were reconsidered and more strict rules were proposed to the infection control committee to eliminate the spread of these strains between wards in our hospital.

Keywords

Author Keywords

Methicillin-resistant Staphyococcus aureus bloodstream infections AP-PCRPFGE

Evaluation of the visual system with visual evoked potential and optical coherence tomography in patients with idiopathic Parkinson's disease and with multiple system atrophy

By

Batum, M (Batum, Melike) [1] ; Ak, AK (Ak, Aysin Kisabay) [1] ; Ari, MS (Ari, Mehmet Semih) [1] ; Mayali, H (Mayali, Huseyin) [2] ; Kurt, E (Kurt, Emin) [2] ; Selçuki, D (Selcuki, Deniz) [1]

 (provided by Clarivate) 

Source

DOCUMENTA OPHTHALMOLOGICA

Volume

145

Issue

2

Page

99-112

DOI

10.1007/s10633-022-09887-7

Published

OCT 2022

Early Access

JUL 2022

Indexed

2022-08-01

Document Type

Article

Jump to

Abstract

Background In addition to motor findings, non-motor findings including alterations in visual acuity, decrease in blink reflex, and pupil reactivity cause the impaired quality of life in idiopathic Parkinson's disease (PD) and multiple system atrophy (MSA). Our study aimed to examine possible latency and amplitude changes in pattern visual evoked potentials (pVEP) along with retinal and macular changes in optical coherence tomography (OCT) in PD and MSA groups. We also intended to investigate whether any OCT parameters could be a biomarker for Parkinson's or MSA. Methods Our study included 50 patients with PD, 15 with MSA, and 50 healthy control subjects. All patients in the study underwent neurological and ophthalmological examination and investigations of OCT to measure the retinal and macular thickness and pVEP to assess visual pathways. Results When PD, MSA, and control groups were compared, a significant difference was found in all retinal thickness values in average, nasal, and superior retinal nerve fiber thickness (pRNFL), and in all macular thickness values except nasal outer and inferior outer quadrants and in ganglion cell complex (GCC) thicknesses (p < 0.05). Moreover, a significant difference was found in N75, P100, and N145 latencies and N75-P100 amplitude (p < 0.05). The thickness of both pRNFL, inner and outer macular quadrants, was thinner in the MSA group than in PD but GCC thickness was thinner in PD group. Conclusions The present study compared pVEP and OCT parameters in PD and MSA groups. It was concluded that pVEP and OCT examinations were of importance in that they were easily accessible, affordable, noninvasive biomarkers that might be used in early periods and progression of the disease and in follow-up.

Keywords

Author Keywords

Idiopathic Parkinson's disease Multiple system atrophy Visual evoked potential Optic coherence tomography Retinal nerve fiber layer Macula

Prognostic factors in patients with cerebral sinus venous thrombosis presenting with hemorrhage and papilledema

By

Saritas, AS (Saritas, Aysegul Seyma) [1] ; Batum, M (Batum, Melike) [1] ; Ataç, C (Atac, Ceyla) [2] ; Öztürk, U (Ozturk, Uzeyir) [1] ; Yilmaz, H (Yilmaz, Hikmet) [1] ; Mavioglu, H (Mavioglu, Hatice) [3] ; Selçuki, D (Selcuki, Deniz) [3] ; Ak, AK (Ak, Aysin Kisabay) [1]

 (provided by Clarivate) 

Source

CLINICAL NEUROLOGY AND NEUROSURGERY

Volume

234

DOI

10.1016/j.clineuro.2023.108013

Article Number

108013

Published

NOV 2023

Early Access

OCT 2023

Indexed

2023-11-26

Document Type

Article

Jump to

Abstract

Objective: The thromboinflammatory process is considered to play a role in the pathogenesis and prognosis of cerebral sinus vein thrombosis (CSVT). The purpose of the study was to compare the thromboinflammatory parameters between individuals with CSVT and healty controls. Additionally, the study sought to compare these parameters among CSVT subgroups with and without haemorrhage, as well as with and without papilledema. Furthermore, the investigation also aimed to identify which parameters had a more significant impact on the risk of hemorrhage and the development of papilledema in CSVT.Methods: Thromboinflammatory parameters were compared retrospectively between the CSVT group of 88 patients and 80 age- and sex-matched healthy controls, and in the CSVT subgroups with (n = 35) /without hemorrhage (n = 53) and with (n = 52) /without papilledema (n = 36) after ethics committee approval. In both groups, parameters contributing to the risk of hemorrhage and papilledema development were determined by univariate and multivariate analyses.Results: Regarding the risk of hemorrhage in CSVT patients, the most significant factors included hematocrit of <30.2 (%), mean platelet volume of <= 8.9 fL, neutrophil count of >5600, and hsCRP of >9 mg/L. Regarding the risk of papilledema development, the most important risk factors were age of <= 49, presence of vomiting, presence of blurred vision, HDL of >47 mg/dL, and D-dimer of >178 ng/mL.Conclusion: It was suggested that evaluating serum thromboinflammatory parameters, as well as demographic characteristics and neurological examination findings, had a critical role regarding prognosis and predictive factors in CSVT.

Author Keywords

Cerebral sinus vein thrombosis Inflammation Thromboinflammatory parameters

CADASIL with Atypical Clinical Symptoms, Magnetic Resonance Imaging, and Novel Mutations: Two Case Reports and a Review of the Literature

By

Sari, US (Sari, U. Serpil) [1] ; Kisabay, A (Kisabay, Aysin) [2] ; Batum, M (Batum, Melike) [2] ; Tarhan, S (Tarhan, Serdar) [3] ; Dogan, N (Dogan, Nihal) [2] ; Coskunoglu, A (Coskunoglu, Aysun) [4] ; Cam, S (Cam, Sirri) [4] ; Yilmaz, H (Yilmaz, Hikmet) [2] ; Selcuki, D (Selcuki, Deniz) [2]

 (provided by Clarivate) 

Source

JOURNAL OF MOLECULAR NEUROSCIENCE

Volume

68

Issue

4

Page

529-538

DOI

10.1007/s12031-019-01313-z

Published

AUG 2019

Indexed

2019-08-02

Document Type

Review

Abstract

Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is a hereditary microangiopathy with adult onset caused by a missense mutation in the NOTCH3 gene in chromosome 19p13. It presents with autosomal dominant arteriopathy, subcortical infarctions, and leukoencephalopathy. Its common clinical presentations are seen as recurrent strokes, migraine or migraine-like headaches, progressive dementia, pseudobulbar paralysis, and psychiatric conditions. Two patients with CADASIL syndrome, whose diagnosis was made based on clinical course, age of onset, imaging findings, and genetic assays in the patients and family members, are presented here because of new familial polymorphisms. The first patient, with cerebellar and psychotic findings, had widespread non-confluent hyperintense lesions as well as moderate cerebellar atrophy in cranial magnetic resonance scanning. The other patient, with headache, dizziness, and forgetfulness, had gliotic lesions in both cerebral hemispheres. CADASIL gene studies revealed a new polymorphism in exon 33 in the first patient. In the other patient, the NOTCH3 gene was identified as a new variant of p.H243P (c.728A>C heterozygous). By reporting a family presenting with various clinical symptoms in the presence of new polymorphisms, we emphasize that CADASIL syndrome may present with various clinical courses and should be considered in differential diagnoses.

Keywords

Author Keywords

CADASILNOTCH3Genetic testing Small vessel disease Headache MRI

Role of acetazolamide in treatment of patients with menstrual migraine

By

Çetin, G (Cetin, Guldeniz) [1] ; Kisabay, AA (Kisabay, Aysin Ak) [1] ; Özyurt, BC (Ozyurt, Beyhan Cengiz) [2] ; Selçuki, D (Selcuki, Deniz) [1]

Source

NEUROLOGY ASIA

Volume

26

Issue

4

Page

751-759

DOI

10.54029/2021hmm

Published

DEC 2021

Indexed

2022-01-18

Document Type

Article

Jump to

Abstract

Objective: According to the ICHD-3 criteria, menstrual migraine (MM) is divided into two groups: pure menstrual migraine (PMM) and menstrually-related migraine (MRM). The present study aimed to evaluate and compare the severity of headache using a visual analog scale (VAS) and the effect on quality of life using the Headache Impact Test (HIT) and Migraine Disability Assessment (MIDAS) tests before and after 3 months of treatment in using short-term prophylaxis with acetazolamide. Methods: Patients who presented to the headache outpatient clinic of the neurology department with a diagnosis of MM were retrospectively reviewed. Acetazolamide was given at a dosage of 500 mg daily for 5 days starting two days before the predicted onset of the menstrual cycle as a short-term prophylactic treatment. VAS, MIDAS, and HIT assessments were performed before and after treatment. Results: A total of 26 patients with PMM and 26 patients with MRM were identified. After acetazolamide treatment, statistically significant improvement was found in MIDAS, VAS and HIT scores in both groups of patients. The post-treatment MIDAS score was significantly lower in the MRM group, but there was no significant difference in post-treatment VAS and HIT scores between the groups. Conclusion: Using acetazolamide for short-term prophylaxis in patients with MM leads to decreased severity and frequency of headache and improvement in quality of life. The study is the first in the literature to use acetazolamide for short-term prophylaxis in patients diagnosed with MM.

Keywords

Author Keywords

menstrual migraine acetazolamide short-term prophylaxis VAS MIDA SHIT

Evaluation of cognitive functions in idiopathic Parkinson's disease and multiple system atrophy

By

Batum, M (Batum, Melike) [1] ; Ak, AK (Ak, Aysin Kisabay) [1] ; Ari, MS (Ari, Mehmet Semih) [1] ; Selçuki, D (Selcuki, Deniz) [1]

 (provided by Clarivate) 

Source

NEUROLOGY ASIA

Volume

26

Issue

1

Page

85-93

Published

MAR 2021

Indexed

2021-05-13

Document Type

Article

Abstract

Background & Objective: Cognitive impairment is one of the non-motor symptoms impairing life quality in idiopathic Parkinson's disease (PD) and multiple system atrophy (MSA). In our study, both groups' possible cognitive impairments were evaluated and compared, and the relationship between cognitive profile and motor, non-motor scores, and disease duration was evaluated. Methods: Fifty two PD, 18 MSA, 30 healthy controls were included in the study. Demographic information, scores of Unified Parkinson's Disease Rating Scale (UPDRS), Parkinson's Disease Questionnaire (PDQ), Addenbrooke's Cognitive Examination-Revised (ACE-R), and Frontal Assessment Battery (FAB) tests were recorded. In addition to the ACE-R test's total scores, sub-scores measuring attention-orientation, memory, verbal fluency, language, and visual-spatial abilities were also evaluated. Results: There was no difference between the groups in age, gender, years of education, and levodopa dose in treatment (p> 0.05). In the inter-group comparison, FAB, ACE-R total, ACE-R sub-scores and PDQ values were significantly different (p <0.05). Significant impairment was found in FAB, ACE-R total, memory, verbal fluency, speaking, and PDQ scores in PD and in all tests in MSA compared to the control group (p <0.05). All tests except memory were more impaired in the MSA group than the PD group. The motor scores in PD showed a strong correlation with FAB, ACE-R total, visual-spatial abilities, speaking, and PDQ scores, whereas motor scores in MSA only correlated poorly with PDQ scores. Conclusion: MSA progressing with multi-systemic involvement showed worse cognitive performance than PD in executive functions and visual-spatial functions, regardless of the disease duration.

Keywords

Author Keywords

Idiopathic Parkinson's disease multiple system atrophy cognition quality of life

Tethered Cord Syndrome in Adults: Experience of 56 Patients

By

Selcuki, M (Selcuki, Mehmet) [1] ; Mete, M (Mete, Mesut) [1] ; Barutcuoglu, M (Barutcuoglu, Mustafa) [1] ; Duransoy, YK (Duransoy, Yusuf Kurtulus) [1] ; Umur, AS (Umur, Ahmet Sukru) [1] ; Selcuki, D (Selcuki, Deniz) [2]

 (provided by Clarivate) 

Source

TURKISH NEUROSURGERY

Volume

25

Issue

6

Page

922-929

DOI

10.5137/1019-5149.JTN.11700-14.1

Published

NOV 2015

Indexed

2015-12-30

Document Type

Article

Abstract

AIM: The aim of this study was to describe the results of surgery performed in a group of adult patients with tethered cord syndrome with their outcomes.

MATERIAL and METHODS: This retrospective study included 56 patients. There were 38 females and 18 males. All patients were older than 18 years.

RESULTS: The mean age at referral was 36 years and 1 month. The mean follow-up period was 10 months 27 days. 95% of all patients with back and leg pains improved and 5% remained the same. Three patients with motor deficits remained the same in the postoperative period. Of the 16 patients with urological complaints, 10 improved, 5 unchanged and 1 patient died in the postoperative first day due to pulmonary embolism.

CONCLUSION: The syndrome of tethered cord may be a situation to be treated even in the elderly in case of normal level conus medullaris and filum terminale with a normal appearance as well as a low-lying conus and thick filum. To prevent overlooking the diagnosis of tethered cord and/or unnecessary spinal surgeries, the tethered cord syndrome should be remembered in the differential diagnosis list in the presence of back and leg pains, neurological deficits or urological complaints.

Keywords

Author Keywords

Tethered cord syndrome Adult Filum terminale Urodynamic study

Evaluation of effects of positive airway pressure treatment on retinal fiber thickness and visual pathways using optic coherence tomography and visual evoked potentials in the patients with severe obstructive sleep apnea syndrome

By

Batum, M (Batum, Melike) [1] ; Kisabay, A (Kisabay, Aysin) [1] ; Mayali, H (Mayali, Huseyin) [2] ; Göktalay, T (Goktalay, Tugba) [3] ; Kurt, E (Kurt, Emin) [2] ; Selçuki, D (Selcuki, Deniz) [1] ; Yilmaz, H (Yilmaz, Hikmet) [1]

 (provided by Clarivate) 

Source

INTERNATIONAL OPHTHALMOLOGY

Volume

40

Issue

10

Page

2475-2485

DOI

10.1007/s10792-020-01426-0

Published

OCT 2020

Early Access

JUN 2020

Indexed

2020-06-19

Document Type

Article

Abstract

Introduction Hypoxia during sleep in obstructive sleep apnea syndrome (OSAS) increases intracranial pressure, decreases cerebral perfusion pressure, and alters vascular supply to the optic nerve. Pattern visual evoked potential (pVEP) has revealed that it causes alterations in the optic nerve, and optic coherence tomography has shown that it causes alterations in the retinal and macular layers. Objectives To detect and compare possible alterations in macula and peripapillary retinal nerve fiber thickness (pRNFL) using OCT and in the optic nerve pathways using pVEP before and after positive airway pressure (PAP) in the patients with severe OSAS. Materials and methods Thirty patients who were diagnosed as having severe OSAS in the neurology-sleep outpatient clinic and 30 healthy control subjects were included in the study. Ophthalmic examinations were performed prior to (month 0) and after (month 6) PAP treatment, and pVEP (peak time [PT] and amplitude) and OCT parameters (peripapillary retinal-macular layers) were compared. Results In the comparison between the severe OSAS (before treatment) and control groups, thinning was found in pRNFL (average, nasal, inferior) and in the macular layers (external and internal superior quadrants) (p < 0.05). pVEP investigation revealed increased PT in P100 and N145 waves and decreased amplitude of N75-P100 waves.

In the comparisons before and after PAP treatment, a decrease in PT of N75 and P100 waves and increase in N75-P100 amplitudes were found. In the pRNFL, significant thickening was found in the layers with thinning before treatment, whereas no significant thickening was found in macular layers, except for the fovea. Discussion It was shown that PAP treatment in patients with severe OSAS prevents hypoxia without causing alterations in intraocular pressure and thus reduces inflammation and causes thickening in the pRNFL and macular layers.

Author Keywords

Peripapillary retinal nerve fiber layer Macula Obstructive sleep apnea syndrome Pattern visual evoked potential Optic coherence tomography Positive airway pressure treatment

Scoliosis may be the first symptom of the tethered spinal cord

By

Barutçuoglu, M (Barutcuoglu, Mustafa) [1] ; Selçuki, M (Selcuki, Mehmet) [1] ; Umur, AS (Umur, Ahmet Sukru) [1] ; Mete, M (Mete, Mesut) [1] ; Gurgen, SG (Gurgen, Seren Gulsen) [2] ; Selcuki, D (Selcuki, Deniz) [3]

 (provided by Clarivate) 

Source

INDIAN JOURNAL OF ORTHOPAEDICS

Volume

50

Issue

1

Page

80-86

DOI

10.4103/0019-5413.173506

Published

JAN-FEB 2016

Indexed

2016-04-13

Document Type

Article

Abstract

Background: Tethered cord syndrome (TCS) is a progressive clinical entity that arises from abnormal spinal cord tension. Scoliosis may be a unique symptom in TCS. The aim of this study is to investigate prognosis after releasing the filum terminale in scoliosis due to TCS with/without findings in magnetic resonance imaging (MRI) and to draw attention to the importance of somatosensorial evoked potentials (SSEP) on the differential diagnosis of idiopathic scoliosis versus scoliosis due to TCS with normal appearance of filum terminale and conus medullaris. Materials and Methods: Eleven female and seven male patients with progressive scoliosis were included in the study. They were evaluated radiologically, SSEP and urodynamical studies. Preoperative and postoperative anteroposterior full spine X-rays were obtained for measuring the Cobb's angle. MRI was performed in all cases for probable additional spinal abnormalities. All patients underwent filum terminale sectioning through a L5 hemilaminectomy. The resected filum terminale were subjected to histopathological examination. Results: The mean Cobb angle was 31.6 degrees (range 18 degrees u45 degrees). Eight patients (44.45%) had a normal appearance of filum terminale and normal level conus medullaris in MRI, but conduction delay and/or block was seen on SSEP. In the histopathological examination of filum terminale dense collagen fibers, hyaline degeneration and loss of elastic fibers were observed. Postoperatively none of the patients showed worsening of the Cobb angle. Three patients showed improvement of scoliosis. Conclusion: In TCS presented with scoliosis, untethering must be performed prior to the corrective spinal surgery. Absence of MRI findings does not definitely exclude TCS. SSEP is an important additional guidance in the diagnosis of TCS. After untethering, a followup period of 6 months is essential to show it untethering helps in stopping the progress of the scoliotic curve. In spite of non progression (curve stopped lesser than 45 degrees) or even improvement of scoliosis, there may be no need for major orthopedic surgical intervention.

Keywords

Author Keywords

Cobb angle scoliosis somatosensorial evoked potentialst ethered cord syndrome

Evaluation of retinal fiber thickness and visual pathways with optic coherence tomography and pattern visual evoked potential in different clinical stages of obstructive sleep apnea syndrome

By

Ak, AK (Ak, Aysin Kisabay) [1] ; Batum, M (Batum, Melike) [1] ; Göktalay, T (Goktalay, Tugba) [2] ; Mayali, H (Mayali, Huseyin) [3] ; Kurt, E (Kurt, Emin) [3] ; Selçuki, D (Selcuki, Deniz) [1] ; Yilmaz, H (Yilmaz, Hikmet) [1]

 (provided by Clarivate) 

Source

DOCUMENTA OPHTHALMOLOGICA

Volume

141

Issue

1

Page

33-43

DOI

10.1007/s10633-020-09749-0

Published

AUG 2020

Early Access

JAN 2020

Indexed

2020-02-03

Document Type

Article

Jump to

Abstract

Purpose To investigate the possible changes in retinal nerve fiber layer (RNFL) by optic coherence tomography and in the amplitudes and peak times (PTs) in pattern visual evoked potential (pVEP) and to compare them in obstructive sleep apnea syndrome (OSAS). Methods This prospective study included patients with mild OSAS (n = 30), severe OSAS (n = 30), and 30 control subjects. All patients were assessed after obtaining the approval from our hospital's ethics committee. Results There was no difference in age and gender between the groups (p = 0.184, p = 0.954). By analysis of variance, there was a significant difference in RNFL values among patients with mild OSAS, severe OSAS, and control for three measures of RNFL (average p = 0.044, nasal p = 0.003, inferior p = 0.027). In severe OSAS group, nasal and inferior quadrants of the RNFL were found to be thinner than the control group (p = 0.008, p = 0.031). We showed that the PT of P100 and N145 was prolonged in severe OSAS compared to the control group (p < 0.001) and that PT of P100 was prolonged in mild OSAS compared to the control group (p < 0.05). The amplitude of N75-P100 was significantly decreased in patients with both severe OSAS and mild OSAS compared to the control group (p < 0.001). Correlation of RNFL and pVEP values showed that the inferior quadrant RNFL thickness is correlated with both P100 and N145 PTs (r = 0.271*, p = 0.036 and r = 0.290*, p = 0.043, respectively) and N75-P100 amplitude (r = 0.378**, p = 0.003) in severe OSAS group. Conclusions In mild and severe stages of the disease, edema and inflammation were evident and VEP PT and amplitudes were affected in both groups. Furthermore, thinning in RNFL in the severe stage of the disease might be associated with higher atrophy levels and prolonged exposure to hypoxia.

Author Keywords

Retinal nerve fiber layer Obstructive sleep apnea syndrome Pattern visual evoked potential Optic coherence tomography

Residual symptoms in bipolar disorder:: The effect of the last episode after remission

By

Kaya, E (Kaya, Ender) ; Aydemir, Ö (Aydemir, Oemer) ; Selcuki, D (Selcuki, Deniz)

 (provided by Clarivate) 

Source

PROGRESS IN NEURO-PSYCHOPHARMACOLOGY & BIOLOGICAL PSYCHIATRY

Volume

31

Issue

7

Page

1387-1392

DOI

10.1016/j.pnpbp.2007.06.003

Published

OCT 1 2007

Indexed

2007-10-01

Document Type

Review

Abstract

In this study it is aimed to assess interepisode residual symptoms in remitted bipolar disorder patients with a hypothesis that the last episode recovered has implications on residual symptomatology. The study was carried out with 23 bipolar patients diagnosed as mania (BP-M) and 20 bipolar patients diagnosed as depression (BP-D) in their last episode, and with 22 healthy controls in a university hospital clinic. All patients were in remission for at least 6 months. In the assessment Hamilton Depression Rating Scale (HAM-D), Young Mania Rating Scale (YMRS), Stroop Test, Auditory Verbal Learning Test (AVLT), increased latency positive-evoked potentials (P300), Global Assessment of Functioning Scale (GAF), and Social Functioning Scale (SFS) were used cross-sectionally. In affective symptomatology, the BP-M group had higher YMRS scores, and the BP-D group had higher HAM-D scores compared to the controls. P300 test results revealed low amplitude in the 13P-D group. In the AVLT, verbal learning and delayed recall were significantly lower in the two bipolar groups. The Stroop tasks were not different in the groups. Concerning the SFS, social withdrawal was impaired in the two bipolar groups, whereas dependency-competency was impaired in the BP-M and employrnent/occupation was impaired in the BP-D group. As a conclusion, bipolar patients recovering from depressive episode may experience more impairment in daily functioning due to residual depressive symptoms and impairment of attention and memory. (c) 2007 Elsevier Inc. All rights reserved.

Keywords

Author Keywords

bipolar disorder cognitive functions residual symptoms social functions
 

Keywords Plus

SOCIAL-ADJUSTMENT NEUROPSYCHOLOGY

AIRPLANE HEADACHE: AN ATYPICAL CASE WITH AUTONOMIC SYMPTOMS AND LONG DURATION

By

Ataç, C (Atac, Ceyla) [1] ; Ak, AK (Ak, Aysin Kisabay) [2] ; Çetin, G (Cetin, Guldeniz) [2] ; Batum, M (Batum, Melike) [2] ; Gökçay, F (Gokcay, Figen) [3] ; Selçuki, D (Selcuki, Deniz) [2]

 (provided by Clarivate) 

Source

NEUROLOGY ASIA

Volume

25

Issue

4

Page

611-613

Published

DEC 2020

Indexed

2021-02-23

Document Type

Article

Abstract

Airplane Headache (AH) which is classified under headache attributed to disorder of homeostasis in International Classification of Headache Disorders (ICHD)-3 is a severe, unilateral, orbitofrontal headache that occurs during and caused by airplane travel. It remits after landing. AH cases with autonomic symptoms had rarely been reported. We present a 35-year-old male complained of five attacks of right-sided, unilateral, orbitofrontal headache accompanied with lacrimation, conjunctival injection and eye redness ipsilaterally, starting 20-30 minutes prior to landing. The headache duration varied between 30-90 minutes. AH diagnosis was made in the light of anamnesis and neurological examination. The secondary causes and primary headaches with autonomic symptoms were ruled out. As far as we know this is the first reported longer duration AH case with autonomic symptoms in the literature. AH is an underdiagnosed headache. We report this atypical AH case to call attention to this rare but treatable headache.

Author Keywords

Airplane Headache autonomic symptoms long duration rare case

Cutting filum terminale is very important in split cord malformation cases to achieve total release

By

Barutcuoglu, M (Barutcuoglu, Mustafa) [1] ; Selcuki, M (Selcuki, Mehmet) [1] ; Selcuki, D (Selcuki, Deniz) [2] ; Umur, S (Umur, Sukru) [1] ; Mete, M (Mete, Mesut) [1] ; Gurgen, SG (Gurgen, Seren Gulsen) [3] ; Umur (Umur) [3]

 (provided by Clarivate) 

Source

CHILDS NERVOUS SYSTEM

Volume

31

Issue

3

Page

425-432

DOI

10.1007/s00381-014-2586-1

Published

MAR 2015

Indexed

2015-03-11

Document Type

Article

Abstract

Split cord malformations (SCMs) are rare congenital anomalies of the vertebrae and the spinal cord. Tethered cord syndrome (TCS) is a clinical condition of various origins that arises from tension on the spinal cord. Radiographic findings may include and/or associate split cord malformations and the other neural tube defects. However, the spinal cord can even be tethered by a filum terminale with normal appearance and normal level conus medullaris in magnetic resonance imaging (MRI). The aim of our study is to show whether SMC patients with normal or abnormal MRI findings had all histological abnormal filum terminale and also to show that the standard SCM repairing operation without cutting filum will not achieve total release.

We have reviewed 33 SCM patients between July 2005 and December 2013. They were operated by adding untethering procedure of filum terminale following standard surgical intervention, and a part of the filum was taken for histopathological examination even though MRI did not show the presence of abnormality of filum terminale.

We found that abnormal filum terminale with a normal appearance may had dense collagen fibers, wide and numerous capillaries, and hyaline formation, while normal filum terminale is a mixture of collagen fibers and blood vessels. We did not obtain positive Verhoeff elastic fiber staining. The elastic fibers had disappeared in all fila terminalia, except control cadaver group.

Our results showed that all fila of SCM patients had loss of elastic fibers and increased of hyalinization, which means loss of elasticity of filum terminale. Less severe traction may remain asymptomatic in childhood and present with neurological dysfunction later in life. For this reason, surgical procedure of SCM patients including releasing of filum terminale seems more beneficial for the patients and be better for long term.

Keywords

Author Keywords

Filum terminale Split cord malformation Tethered cord syndrome Total release

Evaluation of the patients diagnosed as idiopathic intracranial hypertension with and without papilledema visual pathways by analysis of visual evoked potential

By

Kisabay, A (Kisabay, Aysin) [1] ; Selcuki, D (Selcuki, Deniz) [1] ; Zeybek, S (Zeybek, Sinem) [1] ; Batum, M (Batum, Melike) [1]

 (provided by Clarivate) 

Source

INTERNATIONAL JOURNAL OF NEUROSCIENCE

Volume

131

Issue

2

Page

183-190

DOI

10.1080/00207454.2020.1736581

Published

FEB 1 2021

Early Access

MAR 2020

Indexed

2020-04-15

Document Type

Article

Abstract

Background: Idiopathic intracranial hypertension (IIH) is a clinical syndrome characterized by increased intracranial pressure in the absence of clinical, laboratory, or radiological findings of space-occupying lesion in the cranium. Papilledema is found in majority of the patients with IIH while it is absent in only about 5-6% of the patients. Methods: Our primary objective was to evaluate the patients with IIH with (n: 45) and without (n: 15) papilledema using cranial MRI and VEP analyses and to compare the obtained results. Diagnosis of IIH according to ICHD-3 criteria admitted to and followed in our clinic before receiving any treatment between 2008 and 2018 were reviewed retrospectively after obtaining approval from the Ethics Committee. Results: There was no statistically significant difference between both groups' ages (P: 0.494) while differences in lumbar puncture (LP) opening pressure and VAS were found to be statistically significant (p = 0.034, 0.001, respectively). In our VEP investigation, it was seen that latencies in the group without papilledema were seen to be closer to those in the control group (P latency: 0.706, P amplitude: 0.080). Increase in latency and decrease in amplitude were seen in the group with papilledema compared to the group without papilledema (p < 0.001). Conclusion: In conclusion, alterations in the optic nerve may be detected with VEP investigation before the detection of papilledema through ophthalmoscopic examination in these patients. Thus, VEP investigation may be considered to have predictive value. VEP examination may be recommended in diagnosis and treatment and during follow-up periods.

Author Keywords

Demyelination idiopathic intracranial hypertension papilledemaP100visual evoked potential

Are Herniated Cerebellar Tonsils the Main Culprit of Chiari Malformation Type I Symptoms? The Brainstem Compression Hypothesis seems to be Re-Elucidated and Revised

By

Selcuki, M (Selcuki, Mehmet) [1] ; Mete, M (Mete, Mesut) [1] ; Selcuki, D (Selcuki, Deniz) [2]

Source

TURKISH NEUROSURGERY

Volume

28

Issue

1

Page

67-71

DOI

10.5137/1019-5149.JTN.18349-16.1

Published

2018

Indexed

2018-02-06

Document Type

Article

Abstract

AIM: The Chiari Malformation I (CM I) and the tethered cord syndrome (TCS) are both congenital abnormalities whose mechanisms are still not fully understood. The association of CM I and TCS has been reported only a few times previously.

MATERIAL AND METHODS: This retrospective study included 7 patients who were diagnosed with CM I and TCS, managed by cutting of the filum terminale.

RESULTS: The mean follow-up period was 21 months and 28 days. Although all patients underwent an untethering surgical procedure by cutting the filum terminale only, all patients reported significant early postsurgical resolution of CM I symptoms and symptoms related to TCS as well.

CONCLUSION: Patients with symptomatic CM I, even if lumbar MRI is normal and the patient asymptomatic for TCS, may have tethered spinal cord at the same time. It seems it would be worthwhile to investigate CM I patients for occult TCS with spinal somatosensory evoked potentials.

Keywords

Author Keywords

Chiari I Malformation Tethered Cord Syndrome Untethering

Manifestation of Hashimoto's Encephalopathy with Psychotic Signs: a Case Presentation

By

Kisabay, A (Kisabay, Aysin) [1] ; Balikci, K (Balikci, Kuzeymen) [2] ; Sari, S (Sari, Serpil) [1] ; Selcuki, D (Selcuki, Deniz) [1]

 (provided by Clarivate) 

Source

DUSUNEN ADAM-JOURNAL OF PSYCHIATRY AND NEUROLOGICAL SCIENCES

Volume

29

Issue

1

Page

85-90

DOI

10.5350/DAJPN2016290110

Published

MAR 2016

Indexed

2016-10-05

Document Type

Article

Abstract

Hashimoto's encephalopathy (HE) is a rare autoimmune disease with unclear pathophysiology, developing on the basis of Hashimoto's thyroiditis (HT), associated with antithyroid antibodies, that presents with autoimmune, neuroendocrine, and neuropsychiatric findings. The disease is characterized by generalized or focal slowing in the EEG, elevated antithyroid antibody titration in the serum, elevated cerebrospinal fluid protein level, and the presence of antithyroid antibodies. HE is known as an autoimmune disorder of the central nervous system. With this feature, it is differentiated from cognitive alterations observed in hypothyroidism or hyperthyroidism. The response to treatment with corticosteroids in line with the autoimmune nature of HE supports this diagnosis. This report shows a case presenting with neuropsychiatric symptoms, elevated antithyroid antibody titration in the serum, and complete response to steroid therapy. A clear indicator for the diagnosis of HE has not yet been found. Therefore, other potential causes need to be considered in the differential diagnosis of this clinical picture until they can be excluded after investigations. With this case report, we want to emphasize that in differential diagnosis of patients presenting with a variety of neuropsychiatric symptoms, Hashimoto's encephalopathy - though being seen quite rarely - should not be disregarded, given the dramatic improvement of patients receiving a correct diagnosis and appropriate treatment.

Author Keywords

Hashimoto's encephalopathy neuropsychiatric symptomsthyroid antibodies

Investigation of Sleep Breathing Disorders in Young Patients (Under 55 years) with Mild Stroke

By

Kisabay, AKA (Kisabay, Aysin A. K.) [1] ; Saritas, AS (Saritas, Aysegul Seyma) [1] ; Batum, M (Batum, Melike) [1] ; Göktalay, T (Goktalay, Tugba) [2] ; Horasan, GD (Horasan, Gonul Dinc) [3] ; Selcuki, D (Selcuki, Deniz) [1] ; Yilmaz, H (Yilmaz, Hikmet) [1]

 (provided by Clarivate) 

Source

JOURNAL OF STROKE & CEREBROVASCULAR DISEASES

Volume

29

Issue

12

DOI

10.1016/j.jstrokecerebrovasdis.2020.105263

Article Number

105263

Published

DEC 2020

Indexed

2020-12-30

Document Type

Article

Abstract

Introduction: Many patients with stroke also have sleep breathing disorders (SBD), most of which is obstructive sleep apnea (OSA). Methods: Sixty patients with a history of stroke aged under 55 years were included in our study. The demographic characteristics (age at stroke, sex, body mass index) of the patients and etiology of stroke was evaluated according to the Trial of Org 10712 in Acute Stroke (TOAST) classification. The National Institute of Health Stroke Scale (NIHSS) and Modified Rankin Scale (MRS) were used to evaluate functionality. All patients underwent polysomnography (PSG) investigations. Findings: Based on PSG investigations, seven patients were normal, 10 had primary snoring, and 14 had mild, 11 had moderate, and 18 had severe OSA. The demographic characteristics did not differ significantly between the groups. No significant correlation was found between disease severity and NIHSS, MRS or TOAST classifications. Large artery atherosclerosis in the etiology, facial paralysis and dysarthria in the neurological examination, and brain stem involvement in the stroke location were observed to accumulate in the severe OSA group. When the anterior system, posterior system, and association of both were compared for vessel location, no significant difference was found regarding circulatory systems. Conclusions: It is considered that SBD has also a role in the etiology of stroke in patients aged under 55 years, and it should be taken into consideration as much as other risk factors. This is the first study to emphasize the importance of this topic in the literature.

Keywords

Author Keywords

Stroke Polysomnography Sleep breathing disorders Obstructive sleep apnea

Evaluation of visual pathways using visual evoked potentials in the patients with impaired fasting glucose and impaired glucose tolerance

By

Korkmaz, T (Korkmaz, Tugba) [1] ; Kisabay, A (Kisabay, Aysin) [1] ; Hekimsoy, Z (Hekimsoy, Zeliha) [2] ; Senol, E (Senol, Emel) [2] ; Selçuki, D (Selcuki, Deniz) [1]

 (provided by Clarivate) 

Source

INTERNATIONAL JOURNAL OF NEUROSCIENCE

Volume

129

Issue

1

Page

22-29

DOI

10.1080/00207454.2018.1495631

Published

JAN 2 2019

Indexed

2019-03-28

Document Type

Article

Jump to

Abstract

Purpose: There are many studies on degeneration of the ganglion cells using visual evoked potential (VEP) in Diabetes mellitus (DM). The present study intended to investigate whether the retinopathy findings would be helpful for detecting the degeneration to develop or not in retinal ganglion cells with the VEP test before being detectable in ophthalmoscopic examination on prediabetic patients. Materials and methods: The present study was conducted prospectively after obtaining approval from the Ethics Committee. In our study, the subjects were divided into three groups as impaired fasting glucose (IFG), impaired glucose tolerance (IGT) and normal patients. They also underwent physical, ophthalmological and VEP examination. Three main components of VEP obtained from these groups were N75, P100, and N145 latency and N75-P100 amplitude. Results: The study participants consisted of the IFG group (n: 30, female/male ratio: 21/9; mean age: 49.17 +/- 10.52 years), the IGT group (n: 30, female/male ratio: 23/7; mean age: 47.00 +/- 11.09 years), and the Control Group (n: 40, female/male ratio: 30/10; mean age: 48.03 +/- 10.96 years). Difference in sex and age between the study groups (p > 0.05). P100 latency was found to increase significantly in comparison between the IGT and Control Group for both eyes (p right: 0.003, p left: 0.001) whereas it did not increase significantly in the comparison between the IFG and the Control Group (p right: 0.065, p left: 0.116). Conclusion: It was observed that VEP may be a parameter of predictive value that might be used in evaluating prediabetic cases in terms of retinopathies similar to DM.

Author Keywords

Impaired fasting glucose impaired glucose tolerance retinopathy visual evoked potential

Evaluation of the effect of botulinum neurotoxin type A (BoNT/A) on daily activity performance in chronic migraine patients using VAS, MIDAS AND HIT-6 tests

By

Zeybek, S (Zeybek, Sinem) [1] ; Kisabay, A (Kisabay, Aysin) [1] ; Sari, US (Sari, U. Serpil) [1] ; Selcuki, D (Selcuki, Deniz) [1]

Source

NEUROLOGY ASIA

Volume

23

Issue

1

Page

35-43

Published

MAR 2018

Indexed

2018-04-19

Document Type

Article

Abstract

Background & Objectives: According to ICHD-III beta 2013 criteria, chronic migraine is defined as having headaches more than 15 times a month, for a period of more than 3 months, at least 8 must have migrainous features or good response to migraine-specific treatment; there must also be a history of 5 or more migraine attacks. The aim of the present study was to evaluate the effect of Botulinum Neurotoxin A (BONT/A) on headache and daily activities in chronic migraine patients using VAS, MIDAS and HIT-6 tests. Methods: Twenty five patients admitted to Hospital Department of Neurology were reviewed retrospectively. In order to evaluate the severity of headache and effects on daily performance, MIDAS (Migraine Disability Assessment Test), VAS (Visual Analogue Scale for Pain) and HIT-6 results after the baseline assessment, first and second administration of (BONT/A) were examined retrospectively from patients' records. Results: VAS, MIDAS and HIT-6 scores were compared after baseline assessment and the first and second administrations. Results showed that VAS, MIDAS and HIT-6 scores decreased. This difference was statistically significant (p<0.05). Correlation analysis was conducted and significant correlations between scores on these three tests were found.

Conclusions: The results showed that BoNT/A is an important and effective treatment option for chronic migraine patients not responding to migraine-specific prophylactic treatment and having alterations in daily life due to frequency and severity of pain.

Keywords

Author Keywords

Chronic migraine MIDASVASHIT-6botulinum neurotoxin headache

Evaluation of neurodegeneration through visual evoked potentials in restless legs syndrome

By

Kisabay, A (Kisabay, Aysin) [1] ; Sari, US (Sari, Ummu Serpil) [1] ; Korkmaz, T (Korkmaz, Tugba) [1] ; Dinçhorasan, G (Dinchorasan, Gonul) [2] ; Yilmaz, H (Yilmaz, Hikmet) [1] ; Selçuki, D (Selcuki, Deniz) [1]

 (provided by Clarivate) 

Source

ACTA NEUROLOGICA BELGICA

Volume

116

Issue

4

Page

605-613

DOI

10.1007/s13760-016-0631-4

Published

DEC 2016

Indexed

2016-12-07

Document Type

Article

Abstract

Restless legs syndrome (RLS) is a disease characterized by some type of dysesthesia, an indescribable abnormal sensation in the extremities. Our objective was to determine whether the visual evoked potentials (VEP) can be used as a quantitative monitoring method to evaluate demyelination-remyelination and neurodegeneration in the patients with RLS. The present study was carried out prospectively. It was planned to determine normal or pathological conditions in the form of increased latency or decreased amplitude of VEP and to evaluate possible pathologies in the visual and retinal pathways at early stages and at months 3 and 6 of follow-up in the patients with RLS (with or without iron deficiency anemia), in those without RLS (at the time of diagnosis prior to any medical therapy) without any visual symptoms. It was observed that latency of VEP improved but didn't return to normal limits following treatment with dopamin agonists, iron, or combination of both and that there was no significant difference between the post-treatment data and those of the control group. These results in combination with the fact that the latencies and amplitudes didn't return to normal levels despite the 6-month-treatment but showed a progressive course with partial regeneration suggests that there was incomplete remyelination. It should be kept in mind that this syndrome is likely to be a part of neurodegenerative process.

Author Keywords

Restless legs syndrome (RLS)Visual evoked potentials (VEP)Latency Dopamine

Association of myasthenia gravis and Behcet's disease: A case report

By

Kisabay, A (Kisabay, Aysin) [1] ; Sari, US (Sari, Ummu Serpil) [1] ; Boyaci, R (Boyaci, Recep) [1] ; Batum, M (Batum, Melike) [1] ; Yilmaz, H (Yilmaz, Hikmet) [1] ; Selcuki, D (Selcuki, Deniz) [1]

 (provided by Clarivate) 

Source

NEUROLOGIA I NEUROCHIRURGIA POLSKA

Volume

50

Issue

4

Page

284-287

DOI

10.1016/j.pjnns.2016.03.007

Published

2016

Indexed

2016-10-19

Document Type

Article

Abstract

Myasthenia gravis is a disease of neuromuscular junction due to auto-immune destruction of the acetylcholine receptors. Behcet's disease, on the other hand, is a multisystemic vascular-inflammatory disease. Both conditions are not common in the general population although their association has not been reported in the literature. We wanted to present our patient who developed clinical course of myasthenia gravis following discontinuation of medications due to complications of corticosteroid for Behcet's disease. It was observed that clinical findings of myasthenia gravis recovered following restarting steroid treatment and he did not experience attacks of both conditions. Although Myasthenia gravis and Behcet's disease are distinct entities clinically as well as in terms of pathogenesis, they share common physiopathological features and their treatment is based on their common features. (C) 2016 Polish Neurological Society. Published by Elsevier Sp. z o.o. All rights reserved.

Keywords

Author Keywords

Behcet's disease Myasthenia gravis Autoimmune Acetylcholine receptor antibody Corticosteroid