The Effect of Platelet-Rich-Plasma on the Healing of Left Colonic Anastomosis in a Rat Model of Intra-Abdominal Sepsis
By
Sozutek, A (Sozutek, Alper) [1] ; Colak, T (Colak, Tahsin) [2] ; Cetinkunar, S (Cetinkunar, Suleyman) [1] ; Reyhan, E (Reyhan, Enver) [1] ; Irkorucu, O (Irkorucu, Oktay) [1] ; Polat, G (Polat, Gurbuz) [3] ; Cennet, A (Cennet, Ahmet) [4]
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Source
JOURNAL OF INVESTIGATIVE SURGERY
Volume
29
Issue
5
Page
294-301
DOI
10.3109/08941939.2015.1111473
Published
2016
Indexed
2016-10-19
Document Type
Article
Abstract
Objective: The aim of this study was to investigate the effect of platelet-rich plasma (PRP) on the healing of colonic anastomosis in the presence of sepsis. Materials and Method: Fifty Wistar-albino male rats were used. Ten healthy rats were euthanized to prepare PRP, the rest were subjected to colonic anastomosis and randomly allocated into four groups of 10 rats each as anastomosis without PRP (C), without PRP in sepsis (SC), anastomosis with PRP (C-PRP), and with PRP in sepsis (S-PRP). Sepsis was induced by cecal ligation and puncture procedure. All animals were euthanized on postoperative day 7. The body weight change, anastomotic bursting pressure (ABP), tissue hydroxyproline (TH) and histopathological examination of each group were analyzed by using one-way analysis of variance (ANOWA) and Tukey's HSD post-hoc test to assess the differences between the groups. Results: There was no statistical difference among the groups in terms of body weight changes. The ABP was measured at a mean value of 179.5 +/- 10.3, 129.3 +/- 14.2, 209 +/- 14.4, and 167.5 +/- 7.5mm-Hg, in group C, SC, C-PRP, and S-PRP, respectively. The ABP and TH of C-PRP group was significantly higher than three groups (p < .05, for each comparison). In sepsis, PRP significantly raised the mean ABP and TH levels up to the levels of C group. Tissue regeneration was significant with increased collagen formation in C-PRP group than the other groups (p < .05). The healing effect of PRP in the presence of sepsis was significant than S-group (p < .05), while similar to C group (p = .181). Conclusion: PRP application to colonic anastomosis promotes the healing process in rats with intra-abdominal sepsis.
Keywords
Author Keywords
Platelet-rich-plasma colonic anastomosis intra-abdominal sepsis surgery
Investigation of the relationship between periodontal and systemic inflammation in children with Sickle Cell Disease: A case- control study
By
Tonguç, MÖ (Tonguc, Mine Ozturk) [1] ; Öztürk, C (Ozturk, Candan) [2] ; Polat, G (Polat, Gurbuz) [3] ; Bobusoglu, O (Bobusoglu, Onur) [4] ; Tek, SA (Tek, Sebahat Aslan) [2] ; Tasdelen, B (Tasdelen, Bahar) [5] ; Ünal, S (Unal, Selma) [6]
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Source
Volume
149
DOI
10.1016/j.cyto.2021.155724
Article Number
155724
Published
JAN 2022
Early Access
OCT 2021
Indexed
2021-11-17
Document Type
Article
Jump to
Abstract
Periodontal diseases are chronic inflammatory diseases and tissue destruction increases with oxidative stress in periodontal tissues. Periodontal diseases are associated with systemic diseases such as diabetes, cardio-vascular diseases and rheumatoid arthritis by means of systemic inflammation. Sickle cell disease (SCD) is a chronic inflammatory disease in which vaso-occlusive crisis and endothelial dysfunction are present. It is not known whether the chronic systemic inflammation seen in SCD affect periodontal tissues. The aim of this study was to investigate the relationship between periodontal and systemic inflammation in children with SCD. Forty-three children with SCD and 43 healthy children were included in the study. Physical, dental and periodontal statuses were examined, blood and saliva samples were taken. Levels of pro-inflammatory and oxidative stress mediators in serum and saliva were evaluated. The periodontal findings of the groups were similar. The majority of the subjects in both groups had gingival inflammation. In SCD group, significantly higher serum high sensitive C-reactive protein (Hs-CRP), interleukin (IL)-6, IL-8, tumor necrosis factor (TNF)-alpha, total oxidant status (TOS), nitric oxide (NO) and salivary IL-6 were observed (p < 0.05). There were positive correlations between salivary IL-6 levels and serum Hs-CRP levels (r = 0.303, p < 0.05). In addition; it was determined that salivary IL-6, TNF-alpha and NO levels were increased 3-6 times in children with a history of painful crisis or acute chest syndrome compared to children who had never had a painful crisis or acute chest syndrome. Although, observed oral health status was similar in both groups, salivary cytokine levels were increased in children with SCD. The higher salivary cytokine levels may be associated with chronic systemic inflammation and vaso-occlusion observed in
Keywords Sickle cell disease Saliva Cytokine Periodontal disease
A multicenter nationwide reference intervals study for common biochemical analytes in Turkey using Abbott analyzers
By
Ozarda, Y (Ozarda, Yesim) [28] ; Ichihara, K (Ichihara, Kiyoshi) [1] ; Aslan, D (Aslan, Diler) [2] ; Aybek, H (Aybek, Hulya) [2] ; Ari, Z (Ari, Zeki) [3] ; Taneli, F (Taneli, Fatma) [3] ; Coker, C (Coker, Canan) [4] ; Akan, P (Akan, Pinar) [4] ; Sisman, AR (Sisman, Ali Riza) [4] ; Bahceci, O (Bahceci, Onur) [5] ;
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Source
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
Volume
52
Issue
12
Page
1823-1833
DOI
10.1515/cclm-2014-0228
Published
DEC 2014
Indexed
2014-12-17
Document Type
Article
Abstract
Background: A nationwide multicenter study was organized to establish reference intervals (RIs) in the Turkish population for 25 commonly tested biochemical analytes and to explore sources of variation in reference values, including regionality.
Methods: Blood samples were collected nationwide in 28 laboratories from the seven regions (>= 400 samples/region, 3066 in all). The sera were collectively analyzed in Uludag University in Bursa using Abbott reagents and analyzer. Reference materials were used for standardization of test results. After secondary exclusion using the latent abnormal values exclusion method, RIs were derived by a parametric method employing the modified Box-Cox formula and compared with the RIs by the non-parametric method. Three-level nested ANOVA was used to evaluate variations among sexes, ages and regions. Associations between test results and age, body mass index (BMI) and region were determined by multiple regression analysis (MRA).
Results: By ANOVA, differences of reference values among seven regions were significant in none of the 25 analytes. Significant sex-related and age-related differences were observed for 10 and seven analytes, respectively. MRA revealed BMI-related changes in results for uric acid, glucose, triglycerides, high-density lipoprotein (HDL)-cholesterol, alanine aminotransferase, and.-glutamyltransferase. Their RIs were thus derived by applying stricter criteria excluding individuals with BMI >28 kg/m(2). Ranges of RIs by non-parametric method were wider than those by parametric method especially for those analytes affected by BMI.
Conclusions: With the lack of regional differences and the well-standardized status of test results, the RIs derived from this nationwide study can be used for the entire Turkish population.
Keywords
Author Keywords
biochemical parameters common reference intervals multicenter study regional differences
Killer cell immunoglobulin-like receptor genes in patients with breast cancer
By
Ozturk, OG (Ozturk, Ozlem Goruroglu) [1] ; Gun, FD (Gun, Faik Deniz) [2] ; Polat, G (Polat, Gurbuz) [3]
(provided by Clarivate)
Source
Volume
29
Issue
2
Page
511-515
DOI
10.1007/s12032-011-9932-x
Published
JUN 2012
Indexed
2012-05-30
Document Type
Article
Abstract
Killer cell immunoglobulin-like receptors (KIRs) contribute to the pathogenesis of diverse kind of diseases. Previous studies have shown associations between KIR genes, their ligands and either protection or susceptibility to leukemias or virally associated solid tumors. However, the possible roles of KIR gene polymorphisms in the development of breast cancer remain largely unknown. To investigate the association of KIR gene polymorphisms with breast cancer, we carried out the present study on 33 breast cancer patients and 77 healthy controls by means of sequence-specific oligonucleotide probes analysis, and then all data were statistically analyzed by Fisher exact test. Our results showed that the frame genes KIR2DL4, 3DL2, 3DL3, and 3DP1 were found in all patients and all controls. The rate of activating KIR2DS1 was much higher in patients with breast cancer than that in healthy controls (P = 0.032) while the allelic types of activating 2DS4 (2DS4*003/4/6/7) were lower in patients with breast cancer compared with healthy controls (P = 0.028). Additionally, there was a statistically significant negative correlation between 2DL1 genes and breast cancer development (P = 0.025). In conclusion, this study suggests that the activating KIR2DS1 may trigger breast cancer development, while 2DL1 gene and 2DS4*003/4/6/7 alleles are possibly protectors for breast cancer.
Keywords
Breast cancer HLA class IKIRs KIR genes Luminex
A nationwide multicentre study in Turkey for establishing reference intervals of haematological parameters with novel use of a panel of whole blood
By
Ozarda, Y (Ozarda, Yesim) [1] ; Ichihara, K (Ichihara, Kiyoshi) [2] ; Bakan, E (Bakan, Ebubekir) [3] ; Polat, H (Polat, Harun) [3] ; Ozturk, N (Ozturk, Nurinnisa) [3] ; Baygutalp, NK (Baygutalp, Nurcan K.) [3] ; Taneli, F (Taneli, Fatma) [4] ; Guvenc, Y (Guvenc, Yesim) [4] ; Ormen, M (Ormen, Murat) [5] ; Erbayraktar, Z (Erbayraktar, Zubeyde) [5] ;
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Source
Volume
27
Issue
2
Page
350-377
DOI
10.11613/BM.2017.038
Published
2017
Indexed
2017-07-14
Document Type
Article
Abstract
Introduction: A nationwide multicentre study was conducted to establish well-defined reference intervals (Rls) of haematological parameters for the Turkish population in consideration of sources of variation in reference values (RVs).
Materials and methods: K2-EDTA whole blood samples (total of 3363) were collected from 12 laboratories. Sera were also collected for measurements of iron, UIBC, TIBC, and ferritin for use in the latent abnormal values exclusion (LAVE) method. The blood samples were analysed within 2 hours in each laboratory using Cell Dyn and Ruby (Abbott), LH780 (Beckman Coulter), or XT-2000i (Sysmex). A panel of freshly prepared blood from 40 healthy volunteers was measured in common to assess any analyser-dependent bias in the measurements. The SD ratio (SDR) based on ANOVA was used to judge the need for partitioning RVs. Rls were computed by the parametric method with/without applying the LAVE method.
Results: Analyser-dependent bias was found for basophils (Bas), MCHC, RDW and MPV from the panel test results and thus those Rls were derived for each manufacturer. Rls were determined from all volunteers' results for WBC, neutrophils, lymphocytes, monocytes, eosinophils, MCV, MCH and platelets. Gender-specific Rls were required for RBC, haemoglobin, haematocrit, iron, UIBC and ferritin. Region-specific Rls were required for RBC, haemoglobin, haematocrit, UIBC, and TIBC.
Conclusions: With the novel use of a freshly prepared blood panel, manufacturer-specific Rls' were derived for Bas, Bas%, MCHC, RDW and MPV. Regional differences in Rls were observed among the 7 regions of Turkey, which may be attributed to nutritional or environmental factors, including altitude.
Keywords
Author Keywords
multicentre study reference intervals complete blood count haematology Turkey
Relationship between airway colonization, inflammation and exacerbation frequency in COPD
By
Tumkaya, M (Tumkaya, Munir) ; Atis, S (Atis, Sibel) ; Ozge, C (Ozge, Cengiz) ; Delialioglu, N (Delialioglu, Nuran) ; Polat, G (Polat, Gurbuz) ; Kanik, A (Kanik, Arzu)
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Source
Volume
101
Issue
4
Page
729-737
DOI
10.1016/j.rmed.2006.08.020
Published
APR 2007
Indexed
2007-04-01
Document Type
Article
Abstract
Rotionole: To evaluate bacteria[ colonization and the airway inflammatory response, and its relationship to the frequency of exacerbation in patients with stable chronic obstructive pulmonary disease (COPD).
Methods: Quantitative bacteriologic cultures, neutrophil elastase, myeloperoxidase (MPO), tumor necrosis factor alpha (TNF-alpha) and interleukin (IL)-8 were measured in bronchoalveoler lavage (BAL) in 39 patients with stable COPD [19 with frequent exacerbation (>= 3/year), and 20 with infrequent] and in 18 healthy controls (10 smokers and 8 non-smokers).
Results: BAL revealed the microorganisms with potential pathogenicity above the established threshold (>= 10(3) cfu/ml) in 68.4% of patients with frequent exacerbation, 55% of infrequent exacerbation, 40% of smokers and 12.5% of non-smokers controls (P = 0.05). BAL MPO, IL-8 and TNF-alpha levels were found to be significantly higher in COPD as compared to controls (P = 0.001). However, only IL-8 level was significantly higher in COPD patients with frequent exacerbation as compared to infrequent (P = 0.001). Airway bacteria[ toad correlated with levels of airway inflammation markers in COPD (P < 0.05).
Conclusion: The bacterial load and airway inflammation contributes to each other in stable COPD. However, there is a link only between interleukine (IL)-8 and frequent exacerbations. Clearly, the relationship between bacterial colonization, airway inflammation and frequent exacerbations is of major importance in understanding of the COPD pathogenesis. (c) 2006 Elsevier Ltd. All rights reserved.
Keywords
Author Keywords
airway inflammation BAL bacterial colonization COPD exacerbation
TNF-α and IL-1 β Cytokine Gene Polymorphism in Patients with Nasal Polyposis
By
Ismi, O (Ismi, Onur) [1] ; Özcan, C (Ozcan, Cengiz) [1] ; Polat, G (Polat, Gurbuz) [2] ; Kul, S (Kul, Seval) [3] ; Görür, K (Gorur, Kemal) [1] ; Pütürgeli, T (Puturgeli, Tugce) [1]
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Source
TURKISH ARCHIVES OF OTORHINOLARYNGOLOGY
Volume
55
Issue
2
Page
51-56
DOI
10.5152/tao.2017.2389
Published
JUN 2017
Indexed
2017-07-11
Document Type
Article
Abstract
Objective: Nasal Polyp (NP) is a benign mass of the paranasal sinuses that protrudes into the nasal cavity. The exact underlying pathogenesis is not known. In this study we aimed to determine the genetic susceptibility of NP formation in relation to TNF-alpha-308 and IL-1 beta-511 promoter region gene polymorphisms.
Methods: A total of 71 patients with NP with asthma (n=21) or without asthma (n=50) were taken as the study group, and 91 healthy volunteers were taken as the control group. Blood was gathered into EDTA-containing tubes, and patient DNA was extracted. The polymorphisms of the IL-beta and TNF-alpha cytokine genes
Results: The GG genotype in the TNF-alpha-308 region and the CC genotype in the IL-1 beta-511 region were found to be risk factors for NP formation (OR: 9.2, p= 0.007 and OR: 33.3, p= 0.001, respectively). Regarding allelic frequencies, the G allele at the TNF-alpha-308 promoter region was a risk factor for NP formation (OR: 6.06, p<0.001).
Conclusion: TNF-alpha GG genotype in the-308 promoter region and the IL-1 beta CC genotype in the-511 region are genetic risk factors for NP formation.
Keywords
Author Keywords
Nasal polyp cytokine polymorphism genetic TNF-alphaIL-1 beta
Local Effect of Neurotrophin-3 in Neuronal Inflammation of Allergic Rhinitis: Preliminary Report
By
Ismi, O (Ismi, Onur) [1] ; Özcan, C (Ozcan, Cengiz) [1] ; Karabacak, T (Karabacak, Tuba) [2] ; Polat, G (Polat, Gurbuz) [3] ; Vayisoglu, Y (Vayisoglu, Yusuf) [1] ; Güçlütürk, T (Gucluturk, Taylan) [4] ; Görür, K (Gorur, Kemal) [1]
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Source
Volume
32
Issue
4
Page
364-370
DOI
10.5152/balkanmedj.2015.151028
Published
OCT 2015
Indexed
2015-11-04
Document Type
Article
Abstract
Background: Allergic rhinitis is a common inflammatory nasal mucosal disease characterized by sneezing, watery nasal discharge, nasal obstruction and itching. Although allergen-specific antibodies play a main role in the allergic airway inflammation, neuronal inflammation may also contribute to the symptoms of allergic rhinitis. Neuronal inflammation is primarily caused by the stimulation of sensory nerve endings with histamine. It has been shown that neurotrophins may also have a role in allergic reactions and neuronal inflammation. Nerve growth factor, neurotrophin 3 (NT-3), neurotrophin 4/5 and brain-derived neurotrophic factor are members of the neurotrophin family. Although nerve growth factor and brain-derived neurotrophic factor are well studied in allergic rhinitis patients, the exact role of Neurotrophin-3 is not known.
Aims: To investigate the possible roles of neurotrophin- 3 in allergic rhinitis patients.
Study Design: Case-control study.
Methods: Neurotrophin-3 levels were studied in the inferior turbinate and serum samples of 20 allergic rhinitis and 13 control patients. Neurotrophin-3 staining of nasal tissues was evaluated by immunohistochemistry and ELISA was used for the determination of serum Neurotrophin-3 levels.
Results: Neurotrophin-3 staining scores were statistically higher in the study group than in the control patients (p=0.001). Regarding serum Neurotrophin-3 levels, no statistically significant difference could be determined between allergic rhinitis and control patients (p=0.156). When comparing the serum NT-3 levels with tissue staining scores, there were no statistically significant differences in the allergic rhinitis and control groups (p=0.254 for allergic rhinitis and p=0.624 for control groups).
Conclusion: We suggest that Neurotrophin-3 might affect the nasal mucosa locally without being released into the systemic circulation in allergic rhinitis patients.
Keywords
Author Keywords
Airway allergic rhinitis inflammation neuronal neurotrophin-3rhinitis
Vacuum-assisted closure therapy leads to an increase in plasma fibronectin level
By
Arslan, E (Arslan, Emrah) [1] ; Ozturk, OG (Ozturk, Ozlem Goruroglu) [2] ; Aksoy, A (Aksoy, Alper) [3] ; Polat, G (Polat, Gurbuz) [2]
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Source
Volume
8
Issue
3
Page
224-228
DOI
10.1111/j.1742-481X.2011.00772.x
Published
JUN 2011
Indexed
2011-05-27
Document Type
Article
Abstract
An extensive research has been performed to investigate the mechanisms of action by which the application of subatmospheric pressure to wounds increases the rate of healing. Increased blood flow with vacuum-assisted closure (VAC) use is the most popular aspect. Fibronectin, which is an adhesion molecule, has several functional domains mediating chemotaxis, adhesion and migration. This is thereby involved in differentiation, proliferation, inflammation and thus in wound healing. In this study, plasma fibronectin levels were measured before and after VAC in patients with wounds. The results showed that there was an increase in pre- and post-VAC levels of plasma fibronectin. This statistically significant increase could be another explanation of how VAC therapy promotes wound healing.
Keywords
Author Keywords
Plasma fibronectin Vacuum-assisted closure Wound healing
HLA class-II allele frequencies in Turkish breast cancer patients
By
Gun, FD (Gun, Faik Deniz) [2] ; Ozturk, OG (Ozturk, Ozlem Goruroglu) [1] ; Polat, A (Polat, Ayse) [3] ; Polat, G (Polat, Gurbuz) [4]
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Source
Volume
29
Issue
2
Page
466-471
DOI
10.1007/s12032-011-9873-4
Published
JUN 2012
Indexed
2012-05-30
Document Type
Article
Abstract
Class-II human leukocyte antigens (HLA) present tumor antigenic peptides on the cell surface in order to be recognized by T lymphocytes. Thereby, these molecules can play an important role in the immune response to breast cancer tumor antigens. The aim of this study was to determine the relation between breast cancer and HLA class-II alleles in Turkey. The study groups consisted of 69 breast cancer patients and 45 healthy controls. Typing of HLA-DRB1 and HLA-DQB1 from DNA samples was performed by Sequence Specific Oligonucletide Hybridisation. Significant negative correlations were observed between HLA-DRB1*03 and HLA-DQB1*02 alleles and breast cancer (p1 = 0.019; p2 = 0.019) and also between HLA-DQB1*02 allele and postmenopausal breast cancer (P = 0.022) and c-erb-B2 positivity (P = 0.038). Furthermore, there was a significant positive correlation between HLA-DRB1*13 and HLA-DQB1*06 alleles and progesteron receptor positivity (p1 = 0.012; p2 = 0.001); and a significant negative correlation between HLA-DQB1*03 allele and progesteron receptor positivity (P = 0.009) in breast cancer. Additionally, another significant positive correlation was seen between HLA-DRB1*04 allele and c-erb-B2 positivity (P = 0.036). As a result, while HLA-DRB1*03, HLA-DQB1*02, HLA-DRB1*13, and HLA-DQB1*06 alleles were found to be involved in protectiveness against breast cancer and good prognosis; HLA-DQB1*03 and HLA-DRB1*04 alleles were found to be involved in poor prognosis. In conclusion, by determining allele types showing predisposition to breast cancer, a systematical screening and follow up systems can be developed for patients who are at high risk.
Keywords
Author Keywords
Breast cancer HLA class IIHLA-DQHLA-DRB MHC
Intestinal ischemia-reperfusion induced diaphragm contractility dysfunction: Electrophysiological and ultrastructural study in a neonatal rat model
By
Taskinlar, H (Taskinlar, Hakan) [1] ; Nayci, A (Nayci, Ali) [1] ; Çömelekoglu, U (Comelekoglu, Ulku) [2] ; Polat, G (Polat, Gurbuz) [3] ; Zorludemir, S (Zorludemir, Suzan) [4] ; Avlan, D (Avlan, Dincer) [1]
(provided by Clarivate)
Source
Volume
51
Issue
3
Page
354-359
DOI
10.1016/j.jpedsurg.2015.08.013
Published
MAR 2016
Indexed
2016-04-06
Document Type
Article
Abstract
Aim: To evaluate the remote effect of intestinal ischemia reperfusion (IR) injury mediated by tumor necrosis factor alpha (TNF-alpha) on diaphragm contractility functions and whether administration of NAC may counteract the possible detrimental effects in an experimental neonatal rat model.
Methods: 40 Wistar rat pups were randomized into four groups; ten animals in each. Intestinal ischemia was conducted by obstructing mesentery of intestines by a silk loop. In the control group; only laparotomy was performed. After 1 h ischemia, reperfusion was conducted for 1 h in 1 h group, 24 h for 24 h group and 24 h for 24 h + NAC group but administration of NAC (150 mg/kg/day) intraperitoneally twice a day was performed. Inflammatory response was evaluated by tissue TNF-alpha level and contractility functions by mechanic activity studies of the diaphragm. Electrophysiology of the diaphragm and the phrenic nerve was conducted to determine neuropathy or myopathy and transmission electron microscopy was performed to evaluate ultrastructural changes in the phrenic nerve.
Results: Diaphragm tissue TNF-alpha level significantly increased in 1 h and 24 h groups (P = 0.004, P = 0.0001; respectively). Diaphragm mechanic activation force and duration significantly decreased at 1 h and 24 h (P = 0.004, P = 0.02 and P = 0.0001, P = 0.0001; respectively). NAC administration significantly prevented decrease in the maximal contraction and the duration (P < 0.001). Phrenic nerve compound action potential (CMAP) amplitude significantly decreased in 1 h group (P < 0.0001) and NAC administration significantly prevented this decrease when compared with 24 h group (P < 0.001). In diaphragmatic needle electromyography, the duration of motor unit potentials (MUP) was prolonged significantly when compared with control group. Contractility and electrophysiological studies were indicating primarily neuropathy in diaphragm dysfunction. Histopathology revealed axonal and myelin degeneration in the 1 h and 24 h group, but less injury in the NAC administered group.
Conclusions: Intestinal IR induced elevation of TNF-alpha level in the diaphragm. Impairment in the diaphragm contractility and neuropathic changes in the phrenic nerve occurred even in the first hour of reperfusion. NAC administration prevented these detrimental effects. (C) 2016 Elsevier Inc. All rights reserved.
Keywords
Author Keywords
Intestinal ischemia reperfusion Diaphragm Phrenic nerve Neuropathy N-acetyl cysteine
Admission Levels of C-Reactive Protein and Plasminogen Activator Inhibitor-1 in Patients With Acute Myocardial Infarction With and Without Cardiogenic Shock or Heart Failure on Admission
By
Akkus, MN (Akkus, Mehmet Necdet) [1] ; Polat, G (Polat, Gurbuz) [2] ; Yurtdas, M (Yurtdas, Mustafa) [1] ; Akcay, B (Akcay, Burak) [1] ; Ercetin, N (Ercetin, Neslihan) [2] ; Cicek, D (Cicek, Dilek) [1] ; Doven, O (Doven, Oben) [1] ; Sucu, N (Sucu, Nehir) [3]
(provided by Clarivate)
Source
Volume
50
Issue
1
Page
33-45
DOI
10.1536/ihj.50.33
Published
JAN 2009
Indexed
2009-01-01
Document Type
Article
Abstract
Scarce data exist oil die relationship of C-reactive protein (CRP) or Plasminogen activator inhibitor-1 (PAI-1) to the occurrence of heart failure (HF) or cardiogenic shock (CS) after acute myocardial infarction (AMI) and on the relationship between these biomarkers and mortality in CS patients. Thus, we compared high-sensitivity CRP and PAT-1 antigen plasma levels on admission among 3 age- and gender matched AMI patients groups (consisting of 60 patients with CS, 60 with HF, and 60 without HF on admission), after determining that PAI-1 levels did not vary significantly diurnally in these groups by comparing the data among Subgroups Which were divided according, to admission time within the groups. For CS Patients, we also conducted regression analyses to examine the relations of these biomarkers to mortality. CRP levels both in CS (P < 0.001) and HF (P < 0.05) patients were significantly higher compared to those without HF, PAI-1 levels in CS patients were significantly higher compared to both those with (P < 0.05) and without HF (P < 0.01), and CRP and PAI-1 were independent predictors of in-hospital (Odds ratio [OR] = 6.12, 95% confidence intervals [95%CI] = 1.47-25.54 and OR = 5.92, 95%CI = 1.31-26.77, respectively) and 1-year mortality (OR = 5.53, 95%CI = 1.21-25.17 and OR = 5.48, 95%CI = 1.09-27.52, respectively) in CS patients. In conclusion, at admission, CRP is associated with the occurrence of CS and HF and PAI-1 is associated with the occurrence of CS after AMI, and they are of prognostic value in CS complicating AMI. (Int Heart J 2009: 50: 33-45)
Keywords
Author Keywords
Inflammatory processes Fibrinolysis processes Acute coronary syndromes Left ventricular systolic dysfunction Mortality
Diversity of killer cell immunoglobulin-like receptor genes in Southern Turkey
By
Ozturk, OG (Ozturk, Ozlem Goruroglu) [1] ; Polat, G (Polat, Gurbuz) [2] ; Atik, U (Atik, Ugur) [2]
(provided by Clarivate)
Source
Volume
39
Issue
2
Page
1989-1995
DOI
10.1007/s11033-011-0945-5
Published
FEB 2012
Indexed
2012-02-07
Document Type
Article
Abstract
Killer cell immunoglobulin-like receptors (KIRs) are a family of inhibitory and activating receptors expressed by natural killer (NK) cells and regulate NK cells' activity. KIR genes are highly polymorphic markers, characterized by a wide diversity, and can therefore be considered as good population genetic markers. The aim of this study was to determine KIR gene frequencies, ratios of haplotypes and genotypes in Southern Turkey and also to compare the data with other worldwide populations studied previously. The study group consisted of 200 non-related individuals from Southern Turkey. The percentage of each KIR gene in the population group was determined by direct counting. Differences between populations in the distribution of each KIR gene and genotype profile were estimated by two-tailed Fisher Exact test. The most frequent non-framework KIR genes detected in Southern Turkey population were: KIR 2DL1 (97%), KIR 3DL1 (91%), KIR 2DS4 (92%) and the pseudogene 2DP1 (96%). Fourty different genotypes were found in 200 subjects and AA1 genotype was the most frequent (27%). Among 40 different genotypes, ten of these were described for the first time in this study and were added to the database (http://www.allelefrequencies.net) numerized as genotype ID from 400 to 409. Gene frequencies and found genotypes demonstrated similarity of Southern Turkey's KIR repertoire with the KIR repertoires of Middle East and European population. High variability seen in KIR genome in this region is thought to be formed as a result of migration and settlement of different civilizations in this region and heterogenity formed in time.
Keywords
Author Keywords
Killer cell immunoglobulin-like receptors KIR genotypes Turkey population SSOP
Effectiveness of mesalamine and propolis in experimental colitis
By
Aslan, A (Aslan, Ahmet) [3] ; Temiz, M (Temiz, Muhyittin) [3] ; Atik, E (Atik, Esin) [2] ; Polat, G (Polat, Gurbuz) [4] ; Sahinler, N (Sahinler, Nuray) [6] ; Besirov, E (Besirov, Eimir) [5] ; Aban, N (Aban, Nedim) [5] ; Parsak, CK (Parsak, Cem Kaan) [1]
(provided by Clarivate)
Source
Volume
24
Issue
5
Page
1085-1097
DOI
10.1007/BF02877715
Published
SEP-OCT 2007
Indexed
2007-09-01
Document Type
Article
Abstract
This study was conducted to investigate the effects of propolis and mesalamine on experimental colitis in rats. Distal colitis was induced in rats by intracolonic instillation of 2 mL of 4% acetic acid. The animals were randomly assigned to 5 groups: group 1, control, (n=8); group 2, colitis, received no treatment (n=8); group 3, colitis+mesalamine, 2 mL once a day via an enema (n=8); group 4, colitis+propolis, 600 mg/kg once a day via intragastric lavage (n=8); and group 5, colitis+ mesalamine+propolis for 1 wk (n=8). Levels of nitric oxide were statistically significantly different in comparisons between groups 1 and 2, groups 2 and 3, and groups 4 and 5. Malondialdehyde levels were significantly different when group 2 was compared with groups 3, 4, and 5. A significant difference was observed when group 3 was compared with group 4 for myeloperoxidase. Most propolis-treated rats had normal histology; mesalamine-treated and propolis+mesalamine-treated rats had inflammatory cell infiltration at rates of 50% and 33%, respectively. The investigators concluded that propolis and mesalamine are efficient independently and in combination, but that their combined effect was not observed to be additive in experimental colitis.
Keywords
Author Keywords
mesalamine propolis experimental colitis
The effect of remote ischemic preconditioning on healing of colonic anastomoses
By
Colak, T; Turkmenoglu, O; Aydin, S
Source
Volume
143
Issue
2
Page
200-205
DOI
10.1016/j.jss.2006.10.030
Published
DEC 2007
Document Type
Article
Abstract
Background. We aimed to investigate the potential protective effect of remote ischemic preconditioning (IPC) on delayed colonic anastomotic healing induced by remote ischemia and reperfusion (I/R) injury.
Materials and methods. Forty male Wistar rats were randomly assigned into four groups, each consisting of 10 rats: the control group (C), the remote LIR group [I/R, 40 min of superior mesenteric artery (SMA) occlusion], the preconditioned LIR group (IPC, two cycles of 5 min temporary occlusion of SMA before an ischemic insult of 40 min), and the preconditioned group (PC, two cycles of 5 min temporary occlusion of SMA). Colonic anastomosis was performed immediately after the ischemic insult. Anastomotic healing was assessed on postoperative day 7 by determining anastomotic bursting pressure (ABP), tissue hydroxyproline content, histopathological examination, malondialdehyde (MDA), and nitric oxide levels.
Results. Remote LIR injury resulted with significant impairment in anastomotic healing in terms of mean ABP (P = 0.004), hydroxyproline content (P = 0.002), histopathological healing score (P = 0.001), nitric oxide level (P = 0.010), and MDA levels (P = 0.0001) when compared with the control group, but remote IPC did not improve all above mentioned parameters (P = NS for all), except MDA level (P = 0.011) when compared with I/R group. PC alone impaired the ABP (P = 0.0001), but it did not significantly change the other parameters measured (P = NS).
Conclusions. The results of this study showed that remote IPC did not prevent I/R-induced delaying in colonic anastomotic healing. (c) 2007 Elsevier Inc. All rights reserved.
Keywords
Author Keywords
anastomotic healing bursting pressure hydroxyproline nitric oxide ischemia/reperfusion
The role of apoptosis in traumatic versus nontraumatic nasal septal cartilage
By
Görür, K (Gorur, Kemal) ; Polat, G (Polat, Gurbuz) ; Özcan, C (Ozcan, Cengiz) ; Arslan, E (Arslan, Emrah) ; Vayisoglu, Y (Vayisoglu, Yusuf) ; Öztürk, OG (Ozturk, Ozlem Goruroglu) ; Bagdatoglu, O (Bagdatoglu, Ozlen)
(provided by Clarivate)
Source
PLASTIC AND RECONSTRUCTIVE SURGERY
Volume
119
Issue
6
Page
1773-1776
DOI
10.1097/01.prs.0000259089.98720.9f
Published
MAY 2007
Indexed
2007-05-01
Document Type
Article
Abstract
Background: Nasal surgery is occasionally performed to correct traumatic nasal deformity. Septal cartilage is the main tissue to be corrected and is a graft source when needed. A risk in engrafting with cartilage is the possibility of resorption as a result of either necrosis or apoptosis. The authors evaluated the rate of apoptosis in deviated and straight cartilage to investigate the cause of resorption of cartilage tissue.
Methods: Twenty-five patients with traumatic nasal septum deviation (group 1) and 13 patients with nontraumatic nasal septum deviation (group II) were prospectively enrolled. After correction of the deviation, two small samples of cartilage were harvested, one from the deviated site (group la or IIa) and the other from the straight site (group Ib or IIb), immediately frozen at -70 degrees C, and evaluated for apoptosis using DNA agarose gel electrophoresis.
Results: Apoptosis was detected in 14 (56 percent) of the deviated and two (8 percent) of the straight cartilage samples in traumatic patients, whereas it was detected in only one deviated sample (7.7 percent) and none of the straight samples in nontraumatic patients. The apoptosis rates in group la were statistically significant when compared with groups Ib (p = 0.0007) and IIa (p = 0.0007).
Conclusions: The present study demonstrates that apoptosis occurs in traumatized nasal septal cartilage. Apoptosis might be the factor leading to cartilage resorption, weakness, and warping when used as a graft. Thus, cartilage grafting materials should be taken from the nontraumatized portion of the septum and should not be traumatized either during harvesting or before placement.
Keywords
Keywords Plus
CHONDROCYTE APOPTOSIS ARTICULAR-CARTILAGE COMPRESSION NECROSIS DEATH
Evaluation of MHC Class 2 Alleles in Chronic Hepatitis B Patients and Inactive Hepatitis B Carriers
By
Kandemir, Ö (Kandemir, Ozlem) [1] ; Polat, G (Polat, Gurbuz) [2] ; Sayici, T (Sayici, Tuba) [1] ; Görüroglu, Ö (Goruroglu, Ozlem) [2] ; Tasdelen, B (Tasdelen, Bahar) [3]
(provided by Clarivate)
Source
TURKIYE KLINIKLERI TIP BILIMLERI DERGISI
Volume
30
Issue
4
Page
1317-1324
DOI
10.5336/medsci.2009-12020
Published
AUG 2010
Indexed
2010-08-01
Document Type
Article
Abstract
Objective: We aimed to determine MHC Class 2 allele frequencies of chronic hepatitis B cases (CHB) and inactive hepatitis B (IHB) carriers in our population, and to asses the prognosis of disease according to MHC Class 2 type in early stage. Material and Methods: Forty-eight CHB patients, 46 IHB carriers and 100 normal control subjects participiated in this study. DNAs were amplified by PCR using appropriate oligonucleotides. Then, these PCR products were displayed by agarose gel electrophoresis technique by using UV transulliminator. The polymorphism of the alleles HLA-DRB1 and HLA-DQB1 were analyzed based on genotyping including the lengths of the bands displayed on the gel. Results: The IHB carriers had significantly higher frequencies of DQB1*01, DRB1*14 and DQB1*05 alleles than the control subjects. When two groups of patients were compared, the frequencies of HLA-DQB1*01 alleles were significantly higher in the CHB patients than in the IHB carriers. The frequencies of HLA-DRB1*14-*15 and HLA-DQB1*01-*01 genotypes were significantly higher in the CHB patients than in the IHB carriers. Conclusion: It might be advised to determine HLA-DRB1*14 ve HLA-DQB1*01 aliens and HLA-DRB1*14-*15, HLA-DQB1*01-*01 genotypes in the individuals facing with hepatitis B virus, and if the results are positive, these patients should be followed up for chronicity.
Keywords
Author Keywords
Hepatitis B, chronic genes, MHC class II
Effect of propolis on healing in experimental colon anastomosis in rats
By
Temiz, M (Temiz, Muhyittin) [1] ; Aslan, A (Aslan, Ahmet) [1] ; Canbolant, E (Canbolant, Elif) [1] ; Hakverdi, S (Hakverdi, Sibel) [4] ; Polat, G (Polat, Gurbuz) [2] ; Uzun, S (Uzun, Semire) [3] ; Temiz, A (Temiz, Abdulkerim) [5] ; Gonenci, R (Gonenci, Ramazan) [6]
(provided by Clarivate)
Source
Volume
25
Issue
2
Page
159-167
DOI
10.1007/s12325-008-0013-2
Published
FEB 2008
Indexed
2008-02-01
Document Type
Article
Abstract
Introduction: Propolis is the generic name for the resinous substance collected by honeybees, which is known to have antioxidant, anti-inflammatory, apoptosis-inducible effects. Anastomotic dehiscence after colorectal surgery is an important cause of morbidity and mortality. We aimed to assess the effect of propolis on healing in an experimental colon anastomosis in rats.
Methods: Forty adult male Wistar albino rats were randomly assigned into 5 treatment groups with 8 rats in each: Group I, anastomosis+no treatment; Group II, anastomosis+oral propolis ( 600 mg/ kg/ d); Group III, anastomosis+oral ethyl alcohol ( 1 cc/ d); Group IV, anastomosis+rectal propolis ( 600 mg/ kg/ d); Group V, anastomosis+rectal ethyl alcohol ( 1 cc/ d). The bursting pressures, hydroxiproline levels and histopathological changes in each group were measured.
Results: When bursting pressures were compared between groups, we observed that they were increased in the groups treated with propolis in contrast to all other groups. Hydroxiproline levels in the propolis groups were also significantly increased in contrast to the other groups. There was also a statistically significant difference in histopathological changes between the treatment types. When propolis administration methods were compared, we did not observe a statistically significant difference.
Conclusion: Propolis has a significantly favourable effect on healing in experimental colon anastomosis, independent from the method of administration.
Keywords
Author Keywords
anastomosis colon healing propolis
The effect of octreotide on healing of injured colonic anastomosis with immediate postoperative intraperitoneal administration of 5-fluorouracil
By
Colak, T (Colak, Tahsin) ; Dag, A (Dag, Ahmet) ; Turkmenoglu, O (Turkmenoglu, Ozgur) ; Polat, A (Polat, Ayse) ; Comelekoglu, U (Comelekoglu, Ulku) ; Bagdatoglu, O (Bagdatoglu, Ozlen) ; Polat, G (Polat, Gurbuz) ; Akca, T (Akca, Tamer) ; Sucullu, I (Sucullu, Ilker) ; Aydin, S (Aydin, Suha)
(provided by Clarivate)
Source
DISEASES OF THE COLON & RECTUM
Volume
50
Issue
5
Page
660-669
DOI
10.1007/s10350-006-0810-0
Published
MAY 2007
Indexed
2007-05-01
Document Type
Article
Abstract
This study was designed to investigate the effect of octreotide on side effects of immediate usage of 5-fluorouracil after colonic anastomosis. METHODS: Forty male Wistar rats were randomly assigned into four groups and underwent standardized left colonic anastomosis. The rats served as control or received intraperitoneal 5-fluorouracil (20 mg/kg daily), subcutaneous octreotide (20 mu g/kg daily), or both. Diarrhea and wound complications were noted during the experiment. The colonic anastomoses were assessed for healing on postoperative Day 7 by determining the anastomotic bursting pressure, performing histologic examination, and measuring the tissue hydroxyproline content, serum malondialdehyde, and nitric oxide levels. Intraperitoneal adhesions and anastomotic leakage were also noted. RESULTS: No statistical significant difference was found between the control and octreotide groups for each of the parameters measured. Immediate 5-fluorouracil use resulted with higher adhesion score (P = 0.002), significant depression in anastomotic bursting pressure (P=0.0001), histopathologic score (P=0.0001), hydroxyproline content (P=0.0001), and increasing nitric oxide (P=0.0001) and malondialdehyde levels (P=0.0001) compared with the control group. Diarrhea was seen in 80 percent of the 5-fluorouracil group but in neither the control nor octreotide groups (P=0.0001 for each comparison). However, all these parameters were ameliorated by use of concomitant octreotide and 5-fluorouracil (P=0.019, P=0.023, P=0.0001, P=0.006, P=0.0001, and P=0.013, respectively). In addition, diarrhea was found to be prevented (P=0.0001). CONCLUSIONS: The results of this study showed that concomitant octreotide use might prevent the side effects of 5-fluorouracil, such as diarrhea, postoperative adhesion, and delaying the anastomotic healing parameters. In addition, it might reduce tissue damage and inflammation.
Keywords
Author Keywords
anastomotic bursting pressure colonic anastomosis chemotherapy